Genetic Control and Properties of Coli Surface Antigens of Colonization Factor Antigen IV (PCF8775) of Enterotoxigenic Escherichia Coli

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1988 Aug 1

PMID 2456269

Citations 34

Authors

M M McConnell

L V Thomas

G A Willshaw

H R Smith

B Rowe

Affiliations

Soon will be listed here.

Abstract

Enterotoxigenic Escherichia coli producing coli surface antigen 4 (CS4), CS5, and CS6 of colonization factor antigen IV were examined. This factor was originally called putative colonization factor 8775 (PCF8775). All of the coli surface antigens were plasmid coded and were usually carried on the same plasmid as the genes coding for heat-stable toxin (ST) or heat-labile toxin (LT); thus, CS5-CS6-ST, CS6-ST, and CS6-LT plasmids were found. In strains of serotype O25:H42, the genes coding for CS4 and CS6 were on a plasmid separate from that containing the genes coding for ST and LT. CS4 and CS5 were fimbrial antigens with a subunit molecular mass of about 17.0 and 21.0 kilodaltons (kDa), respectively. CS6 was found as a single polypeptide with a molecular mass of about 14.5 kDa in strains of serotypes O25:H42, O27:H7, and O27:H20 when heated extracts were run on sodium dodecyl sulfate-polyacrylamide gels. CS6-positive extracts of strains of serogroups O148, O159, and O167 showed two bands with molecular masses between 14.5 and 16.0 kDa.

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References

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Svennerholm A, Vidal Y, Holmgren J, McConnell M, Rowe B . Role of PCF8775 antigen and its coli surface subcomponents for colonization, disease, and protective immunogenicity of enterotoxigenic Escherichia coli in rabbits. Infect Immun. 1988; 56(2):523-8. PMC: 259314. DOI: 10.1128/iai.56.2.523-528.1988. View

Donta S, Moon H, Whipp S . Detection of heat-labile Escherichia coli enterotoxin with the use of adrenal cells in tissue culture. Science. 1974; 183(4122):334-6. DOI: 10.1126/science.183.4122.334. View

Evans D, Silver R, Evans Jr D, Chase D, Gorbach S . Plasmid-controlled colonization factor associated with virulence in Esherichia coli enterotoxigenic for humans. Infect Immun. 1975; 12(3):656-67. PMC: 415337. DOI: 10.1128/iai.12.3.656-667.1975. View

Evans D, Evans Jr D, Tjoa W, DuPont H . Detection and characterization of colonization factor of enterotoxigenic Escherichia coli isolated from adults with diarrhea. Infect Immun. 1978; 19(2):727-36. PMC: 414140. DOI: 10.1128/iai.19.2.727-736.1978. View

Evans D, Evans Jr D . New surface-associated heat-labile colonization factor antigen (CFA/II) produced by enterotoxigenic Escherichia coli of serogroups O6 and O8. Infect Immun. 1978; 21(2):638-47. PMC: 422040. DOI: 10.1128/iai.21.2.638-647.1978. View

Evans D, Evans Jr D, Clegg S, Pauley J . Purification and characterization of the CFA/I antigen of enterotoxigenic Escherichia coli. Infect Immun. 1979; 25(2):738-48. PMC: 414505. DOI: 10.1128/iai.25.2.738-748.1979. View

Birnboim H, DOLY J . A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979; 7(6):1513-23. PMC: 342324. DOI: 10.1093/nar/7.6.1513. View

Willshaw G, Smith H, Anderson E . Application of agarose gel electrophoresis to the characterization of plasmid DNA in drug-resistant enterobacteria. J Gen Microbiol. 1979; 114(1):15-25. DOI: 10.1099/00221287-114-1-15. View

10.

McConnell M, Smith H, Willshaw G, Field A, Rowe B . Plasmids coding for colonization factor antigen I and heat-stable enterotoxin production isolated from enterotoxigenic Escherichia coli: comparison of their properties. Infect Immun. 1981; 32(2):927-36. PMC: 351530. DOI: 10.1128/iai.32.2.927-936.1981. View

11.

Cravioto A, Scotland S, Rowe B . Hemagglutination activity and colonization factor antigens I and II in enterotoxigenic and non-enterotoxigenic strains of Escherichia coli isolated from humans. Infect Immun. 1982; 36(1):189-97. PMC: 351202. DOI: 10.1128/iai.36.1.189-197.1982. View

12.

Willshaw G, Smith H, McConnell M, Barclay E, Krnjulac J, Rowe B . Genetic and molecular studies of plasmids coding for colonization factor antigen I and heat-stable enterotoxin in several escherichia coli serotypes. Infect Immun. 1982; 37(3):858-68. PMC: 347617. DOI: 10.1128/iai.37.3.858-868.1982. View

13.

Thomas L, Rowe B . The occurrence of colonisation factors (CFA/I, CFA/II and E8775) in enterotoxigenic Escherichia coli from various countries in South East Asia. Med Microbiol Immunol. 1982; 171(2):85-90. DOI: 10.1007/BF02124915. View

14.

SMYTH C . Two mannose-resistant haemagglutinins on enterotoxigenic Escherichia coli of serotype O6:K15:H16 or H-isolated from travellers' and infantile diarrhoea. J Gen Microbiol. 1982; 128(9):2081-96. DOI: 10.1099/00221287-128-9-2081. View

15.

Darfeuille A, Lafeuille B, Joly B, CLUZEL R . A new colonization factor antigen (CFA/III) produced by enteropathogenic Escherichia coli O128:B12. Ann Microbiol (Paris). 1983; 134A(1):53-64. DOI: 10.1016/0769-2609(83)90103-5. View

16.

Honda T, Arita M, MIWATANI T . Characterization of new hydrophobic pili of human enterotoxigenic Escherichia coli: a possible new colonization factor. Infect Immun. 1984; 43(3):959-65. PMC: 264278. DOI: 10.1128/iai.43.3.959-965.1984. View

17.

Mullany P, Field A, McConnell M, Scotland S, Smith H, Rowe B . Expression of plasmids coding for colonization factor antigen II (CFA/II) and enterotoxin production in Escherichia coli. J Gen Microbiol. 1983; 129(12):3591-601. DOI: 10.1099/00221287-129-12-3591. View

18.

Levine M, Ristaino P, Marley G, Smyth C, Knutton S, Boedeker E . Coli surface antigens 1 and 3 of colonization factor antigen II-positive enterotoxigenic Escherichia coli: morphology, purification, and immune responses in humans. Infect Immun. 1984; 44(2):409-20. PMC: 263534. DOI: 10.1128/iai.44.2.409-420.1984. View

19.

Manning P, Timmis K, Stevenson G . Colonization factor antigen II (CFA/II) of enterotoxigenic Escherichia coli: molecular cloning of the CS3 determinant. Mol Gen Genet. 1985; 200(2):322-7. DOI: 10.1007/BF00425443. View

20.

McConnell M, Thomas L, Day N, Rowe B . Enzyme-linked immunosorbent assays for the detection of adhesion factor antigens of enterotoxigenic Escherichia coli. J Infect Dis. 1985; 152(6):1120-7. DOI: 10.1093/infdis/152.6.1120. View