Interplay Between Endothelin and Erythropoietin in Astroglia: the Role in Protection Against Hypoxia

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2014 Feb 22

PMID 24557580

Citations 5

Authors

Richard Schafer

Lars Mueller

Reinhild Buecheler

Barbara Proksch

Matthias Schwab

Christoph H Gleiter

Lusine Danielyan

Affiliations

Soon will be listed here.

Abstract

We show that, under in vitro conditions, the vulnerability of astroglia to hypoxia is reflected by alterations in endothelin (ET)-1 release and capacity of erythropoietin (EPO) to regulate ET-1 levels. Exposure of cells to 24 h hypoxia did not induce changes in ET-1 release, while 48-72 h hypoxia resulted in increase of ET-1 release from astrocytes that could be abolished by EPO. The endothelin receptor type A (ETA) antagonist BQ123 increased extracellular levels of ET-1 in human fetal astroglial cell line (SV-FHAS). The survival and proliferation of rat primary astrocytes, neural precursors, and neurons upon hypoxic conditions were increased upon administration of BQ123. Hypoxic injury and aging affected the interaction between the EPO and ET systems. Under hypoxia EPO decreased ET-1 release from astrocytes, while ETA receptor blockade enhanced the expression of EPO mRNA and EPO receptor in culture-aged rat astroglia. The blockade of ETA receptor can increase the availability of ET-1 to the ETB receptor and can potentiate the neuroprotective effects of EPO. Thus, the new therapeutic use of combined administration of EPO and ETA receptor antagonists during hypoxia-associated neurodegenerative disorders of the central nervous system (CNS) can be suggested.

Citing Articles

Targeting Endothelin in Alzheimer's Disease: A Promising Therapeutic Approach.

Sharma S, Behl T, Kumar A, Sehgal A, Singh S, Sharma N Biomed Res Int. 2021; 2021:7396580.

PMID: 34532504 PMC: 8440097. DOI: 10.1155/2021/7396580.

Neuroprotective, Neurogenic, and Amyloid Beta Reducing Effect of a Novel Alpha 2-Adrenoblocker, Mesedin, on Astroglia and Neuronal Progenitors upon Hypoxia and Glutamate Exposure.

Melkonyan M, Hunanyan L, Lourhmati A, Layer N, Beer-Hammer S, Yenkoyan K Int J Mol Sci. 2017; 19(1).

PMID: 29267189 PMC: 5795961. DOI: 10.3390/ijms19010009.

Effects of erythropoietin and methylprednisolone on AQP4 expression in astrocytes.

Wang C, Xu Y, Huang Y, Huang Y Mol Med Rep. 2017; 16(5):5924-5930.

PMID: 28849166 PMC: 5865770. DOI: 10.3892/mmr.2017.7330.

Regeneration in the nervous system with erythropoietin.

Maiese K Front Biosci (Landmark Ed). 2015; 21(3):561-596.

PMID: 26549969 PMC: 4632844. DOI: 10.2741/4408.

Novel applications of trophic factors, Wnt and WISP for neuronal repair and regeneration in metabolic disease.

Maiese K Neural Regen Res. 2015; 10(4):518-28.

PMID: 26170801 PMC: 4424733. DOI: 10.4103/1673-5374.155427.

References

Quaschning T, Ruschitzka F, Stallmach T, Shaw S, Morawietz H, Goettsch W . Erythropoietin-induced excessive erythrocytosis activates the tissue endothelin system in mice. FASEB J. 2002; 17(2):259-61. DOI: 10.1096/fj.02-0296fje. View

Khamaisi M, Dahan R, Hamed S, Abassi Z, Heyman S, Raz I . Role of protein kinase C in the expression of endothelin converting enzyme-1. Endocrinology. 2008; 150(3):1440-9. DOI: 10.1210/en.2008-0524. View

Kleeberg J, Petzold G, Major S, Dirnagl U, Dreier J . ET-1 induces cortical spreading depression via activation of the ETA receptor/phospholipase C pathway in vivo. Am J Physiol Heart Circ Physiol. 2003; 286(4):H1339-46. DOI: 10.1152/ajpheart.00227.2003. View

McWhinnie R, Pechkovsky D, Zhou D, Lane D, Halayko A, Knight D . Endothelin-1 induces hypertrophy and inhibits apoptosis in human airway smooth muscle cells. Am J Physiol Lung Cell Mol Physiol. 2006; 292(1):L278-86. DOI: 10.1152/ajplung.00111.2006. View

Ehrenreich H, Nau T, Dembowski C, Hasselblatt M, Barth M, Hahn A . Endothelin b receptor deficiency is associated with an increased rate of neuronal apoptosis in the dentate gyrus. Neuroscience. 2000; 95(4):993-1001. DOI: 10.1016/s0306-4522(99)00507-2. View

Ameln H, Gustafsson T, Sundberg C, Okamoto K, Jansson E, Poellinger L . Physiological activation of hypoxia inducible factor-1 in human skeletal muscle. FASEB J. 2005; 19(8):1009-11. DOI: 10.1096/fj.04-2304fje. View

Kobari M, Fukuuchi Y, Tomita M, Tanahashi N, Konno S, Takeda H . Dilatation of cerebral microvessels mediated by endothelin ETB receptor and nitric oxide in cats. Neurosci Lett. 1994; 176(2):157-60. DOI: 10.1016/0304-3940(94)90071-x. View

Gandhi C, Nemoto E, Watkins S, Subbotin V . An endothelin receptor antagonist TAK-044 ameliorates carbon tetrachloride-induced acute liver injury and portal hypertension in rats. Liver. 1998; 18(1):39-48. DOI: 10.1111/j.1600-0676.1998.tb00125.x. View

Desai D, He S, Yorio T, Krishnamoorthy R, Prasanna G . Hypoxia augments TNF-alpha-mediated endothelin-1 release and cell proliferation in human optic nerve head astrocytes. Biochem Biophys Res Commun. 2004; 318(3):642-8. DOI: 10.1016/j.bbrc.2004.04.073. View

10.

Nakamura S, Naruse M, Naruse K, Shioda S, Nakai Y, Uemura H . Colocalization of immunoreactive endothelin-1 and neurohypophysial hormones in the axons of the neural lobe of the rat pituitary. Endocrinology. 1993; 132(2):530-3. DOI: 10.1210/endo.132.2.8425473. View

11.

Danielyan L, Lourhmati A, Verleysdonk S, Kabisch D, Proksch B, Thiess U . Angiotensin receptor type 1 blockade in astroglia decreases hypoxia-induced cell damage and TNF alpha release. Neurochem Res. 2007; 32(9):1489-98. DOI: 10.1007/s11064-007-9337-6. View

12.

Earley S, Nelin L, Chicoine L, Walker B . Hypoxia-induced pulmonary endothelin-1 expression is unaltered by nitric oxide. J Appl Physiol (1985). 2002; 92(3):1152-8. DOI: 10.1152/japplphysiol.00829.2001. View

13.

Lendahl U, Zimmerman L, McKay R . CNS stem cells express a new class of intermediate filament protein. Cell. 1990; 60(4):585-95. DOI: 10.1016/0092-8674(90)90662-x. View

14.

Yagami T, Ueda K, Asakura K, Kuroda T, Hata S, Sakaeda T . Effects of endothelin B receptor agonists on amyloid beta protein (25-35)-induced neuronal cell death. Brain Res. 2002; 948(1-2):72-81. DOI: 10.1016/s0006-8993(02)02951-7. View

15.

Ehrenreich H, Fischer B, Norra C, Schellenberger F, Stender N, Stiefel M . Exploring recombinant human erythropoietin in chronic progressive multiple sclerosis. Brain. 2007; 130(Pt 10):2577-88. DOI: 10.1093/brain/awm203. View

16.

Danielyan L, Schafer R, Schulz A, Ladewig T, Lourhmati A, Buadze M . Survival, neuron-like differentiation and functionality of mesenchymal stem cells in neurotoxic environment: the critical role of erythropoietin. Cell Death Differ. 2009; 16(12):1599-614. DOI: 10.1038/cdd.2009.95. View

17.

Blomstrand F, Giaume C, Hansson E, Ronnback L . Distinct pharmacological properties of ET-1 and ET-3 on astroglial gap junctions and Ca(2+) signaling. Am J Physiol. 1999; 277(4):C616-27. DOI: 10.1152/ajpcell.1999.277.4.C616. View

18.

Danielyan L, Mueller L, Proksch B, Kabisch D, Weller M, Wiesinger H . Similar protective effects of BQ-123 and erythropoietin on survival of neural cells and generation of neurons upon hypoxic injury. Eur J Cell Biol. 2005; 84(11):907-13. DOI: 10.1016/j.ejcb.2005.07.001. View

19.

Digicaylioglu M, Kaul M, Fletcher L, Dowen R, Lipton S . Erythropoietin protects cerebrocortical neurons from HIV-1/gp120-induced damage. Neuroreport. 2004; 15(5):761-3. DOI: 10.1097/00001756-200404090-00004. View

20.

Rafiee P, Shi Y, Su J, Pritchard Jr K, Tweddell J, Baker J . Erythropoietin protects the infant heart against ischemia-reperfusion injury by triggering multiple signaling pathways. Basic Res Cardiol. 2004; 100(3):187-97. DOI: 10.1007/s00395-004-0508-1. View