BlaKPC Gene Detection in Clinical Isolates of Carbapenem Resistant Enterobacteriaceae in a Tertiary Care Hospital

Overview

Journal J Clin Diagn Res

Specialty General Medicine

Date 2014 Feb 20

PMID 24551626

Citations 16

Authors

Priyadarshini Shanmugam

Jeya Meenakshisundaram

Perumal Jayaraman

Affiliations

Soon will be listed here.

Abstract

Introduction: Carbapenem resistance among Enterobacteriaceae, especially in Klebsiella pneumoniae and Escherichia coli, is an emerging problem worldwide. A common mechanism of carbapenem resistance is the production of class-A, Klebsiella pneumoniae carbapenemase (KPC).

Aims And Objectives: The present study focused on determining the antibiotic resistance pattern and prevalence of bla KPC gene coding for KPC in carbapenem resistant Enterobacteriaceae.

Methodology: Forty six carbapenem resistant isolates belonging to the family Enterobacteriaceae were tested for antibiotic sensitivity pattern. Modified Hodge Test (MHT) and PCR for bla KPC gene detection were performed on these isolates. Of these, 22 were Klebsiella pneumoniae, 21 were Escherichia coli, 2 were Citrobacter species and 1 was Proteus mirabilis Results: Forty three (93.4%) out of the 46 isolates were resistant to Meropenem, 34 (73.9%) were resistant to Imipenem and 30 (65.2%) were resistant to both Imipenem and Meropenem. Modified Hodge Test was positive in 38 (82.6%) out of 46 isolates and blaKPC gene was detected in 31 (67.4%) isolates. bla KPC gene was detected in 28 out of the 38 MHT positive isolates.

Citing Articles

Klebsiella pneumoniae carbapenamases in Escherichia coli isolated from humans and livestock in rural south-western Uganda.

Tuhamize B, Asiimwe B, Kasaza K, Sabiiti W, Holden M, Bazira J PLoS One. 2023; 18(7):e0288243.

PMID: 37440483 PMC: 10343067. DOI: 10.1371/journal.pone.0288243.

Rapid detection of carbapenem resistance among gram-negative organisms directly from positive blood culture bottles.

Kumar M, Tandel K, Shergill S, Bhalla G, Mahajan P, Swarnim V Med J Armed Forces India. 2023; 79(3):267-274.

PMID: 37193528 PMC: 10182285. DOI: 10.1016/j.mjafi.2021.03.026.

Effect of Colistin, Fosfomycin and Meropenem/Vaborbactam on Carbapenem-Resistant in Egypt: A Cross-Sectional Study.

Shrief R, El-Ashry A, Mahmoud R, El-Mahdy R Infect Drug Resist. 2022; 15:6203-6214.

PMID: 36324668 PMC: 9621046. DOI: 10.2147/IDR.S385411.

Fingolimod Promotes Antibacterial Effect of Doripenem against Carbapenem-Resistant .

Jin H, Kim H, Eom Y Antibiotics (Basel). 2022; 11(8).

PMID: 36009912 PMC: 9405376. DOI: 10.3390/antibiotics11081043.

Prevalence of carbapenemase-producing Klebsiella pneumoniae at a tertiary care hospital in Kathmandu, Nepal.

Pyakurel S, Ansari M, Kattel S, Rai G, Shrestha P, Rai K Trop Med Health. 2021; 49(1):78.

PMID: 34565485 PMC: 8474737. DOI: 10.1186/s41182-021-00368-2.

References

Cabral A, Melo R, Maciel M, Lopes A . Multidrug resistance genes, including bla(KPC) and bla(CTX)-M-2, among Klebsiella pneumoniae isolated in Recife, Brazil. Rev Soc Bras Med Trop. 2012; 45(5):572-8. DOI: 10.1590/s0037-86822012000500007. View

Queenan A, Bush K . Carbapenemases: the versatile beta-lactamases. Clin Microbiol Rev. 2007; 20(3):440-58, table of contents. PMC: 1932750. DOI: 10.1128/CMR.00001-07. View

Nagaraj S, Chandran S, Shamanna P, Macaden R . Carbapenem resistance among Escherichia coli and Klebsiella pneumoniae in a tertiary care hospital in south India. Indian J Med Microbiol. 2012; 30(1):93-5. DOI: 10.4103/0255-0857.93054. View

Papp-Wallace K, Endimiani A, Taracila M, Bonomo R . Carbapenems: past, present, and future. Antimicrob Agents Chemother. 2011; 55(11):4943-60. PMC: 3195018. DOI: 10.1128/AAC.00296-11. View

Raghunathan A, Samuel L, Tibbetts R . Evaluation of a real-time PCR assay for the detection of the Klebsiella pneumoniae carbapenemase genes in microbiological samples in comparison with the modified Hodge test. Am J Clin Pathol. 2011; 135(4):566-71. DOI: 10.1309/AJCPPVNUI3O9JHJW. View

Mosca A, Miragliotta L, Del Prete R, Tzakis G, Dalfino L, Bruno F . Rapid and sensitive detection of bla KPC gene in clinical isolates of Klebsiella pneumoniae by a molecular real-time assay. Springerplus. 2013; 2(1):31. PMC: 3581762. DOI: 10.1186/2193-1801-2-31. View

Deshpande L, Rhomberg P, Sader H, Jones R . Emergence of serine carbapenemases (KPC and SME) among clinical strains of Enterobacteriaceae isolated in the United States Medical Centers: report from the MYSTIC Program (1999-2005). Diagn Microbiol Infect Dis. 2006; 56(4):367-72. DOI: 10.1016/j.diagmicrobio.2006.07.004. View

Arnold R, Thom K, Sharma S, Phillips M, Johnson J, Morgan D . Emergence of Klebsiella pneumoniae carbapenemase-producing bacteria. South Med J. 2010; 104(1):40-5. PMC: 3075864. DOI: 10.1097/SMJ.0b013e3181fd7d5a. View

ANDERSON K, Lonsway D, Rasheed J, Biddle J, Jensen B, McDougal L . Evaluation of methods to identify the Klebsiella pneumoniae carbapenemase in Enterobacteriaceae. J Clin Microbiol. 2007; 45(8):2723-5. PMC: 1951220. DOI: 10.1128/JCM.00015-07. View

10.

Marschall J, Tibbetts R, Dunne Jr W, Frye J, Fraser V, Warren D . Presence of the KPC carbapenemase gene in Enterobacteriaceae causing bacteremia and its correlation with in vitro carbapenem susceptibility. J Clin Microbiol. 2008; 47(1):239-41. PMC: 2620828. DOI: 10.1128/JCM.02123-08. View

11.

Walther-Rasmussen J, Hoiby N . Class A carbapenemases. J Antimicrob Chemother. 2007; 60(3):470-82. DOI: 10.1093/jac/dkm226. View

12.

Hindiyeh M, Smollen G, Grossman Z, Ram D, Davidson Y, Mileguir F . Rapid detection of blaKPC carbapenemase genes by real-time PCR. J Clin Microbiol. 2008; 46(9):2879-83. PMC: 2546719. DOI: 10.1128/JCM.00661-08. View

13.

Francis R, Wu F, Della-Latta P, Shi J, Whittier S . Rapid detection of Klebsiella pneumoniae carbapenemase genes in enterobacteriaceae directly from blood culture bottles by real-time PCR. Am J Clin Pathol. 2012; 137(4):627-32. DOI: 10.1309/AJCP9SNHJG2QGLWU. View