Structural Characterization and Immunomodulatory Effect of a Polysaccharide HCP-2 from Houttuynia Cordata

Overview

Journal Carbohydr Polym

Specialty Biochemistry

Date 2014 Feb 18

PMID 24528726

Citations 8

Authors

Bao-Hui Cheng

Judy Yuet-Wa Chan

Ben Chung-Lap Chan

Huang-Quan Lin

Xiao-Qiang Han

Xuelin Zhou

David Chi-Cheong Wan

Yi-Fen Wang

Ping-Chung Leung

Kwok-Pui Fung

Clara Bik-San Lau

Affiliations

Soon will be listed here.

Abstract

Immunomodulation of natural polysaccharides has been the hot topic of research in recent years. In order to explore the immunomodulatory effect of Houttuynia cordata Thunb., the water extract was studied and a polysaccharide HCP-2 with molecular weight of 60,000 Da was isolated by chromatography using DEAE Sepharose CL-6B and Sephacryl S-500 [corrected] HR columns. The structure characterization of HCP-2 was performed by Fourier transform infrared spectroscopy (FTIR), acidic hydrolysis, PMP derivation, HPLC analysis and nuclear magnetic resonance spectra (NMR). HCP-2 was elucidated as a pectic polysaccharide with a linear chain of 1,4-linked α-D-galacturonic acid residues in which part of the 6-carboxyl groups were methyl esterified and part of 2-hydroxyl groups were acetylated. The bioactivity assays showed that HCP-2 could increase the secretions of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), macrophage inhibitory protein-1α (MIP-1α), macrophage inhibitory protein-1β (MIP-1β), and RANTES (regulated on activation, normal T cell expressed and secreted) in human peripheral blood mononuclear cells (PBMCs), which play critical roles in the innate immune system and shape the adaptive immunity. Our results implied that HCP-2 could be an immune enhancer.

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