VH Gene Family Utilization is Regulated by a Locus Outside of the VH Region

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1988 Apr 1

PMID 2451707

Citations 10

Authors

G E Wu

C J Paige

Affiliations

Soon will be listed here.

Abstract

We have used the RNA colony blot method to examine VH usage in colonies derived from primary splenic B cells. We found that there are strain-specific differences in the pattern of VH usage. Using parental F1, congenic, and recombinant inbred strains we demonstrate that the genetic element that causes the observed phenotype is: (a) stably expressed; (b) not due to maternal influence; (c) not due to dominate diffusible factors; (d) not linked to cloning efficiency; and (e) outside the Igh locus.

Citing Articles

The organization of the mouse Igh-V locus. Dispersion, interspersion, and the evolution of VH gene family clusters.

Brodeur P, Osman G, Mackle J, Lalor T J Exp Med. 1988; 168(6):2261-78.

PMID: 3199068 PMC: 2189169. DOI: 10.1084/jem.168.6.2261.

Infrequent utilization of the immunoglobulin heavy chain variable region(s) identical or closely related to that of MOPC315 myeloma protein in the functional V region formation in B-precursor cell lines.

Sugiyama H, Minami Y, Komori T, Sakato N, Kishimoto S Immunology. 1989; 68(4):453-7.

PMID: 2514136 PMC: 1385529.

Molecular cloning of the primary IgH repertoire: a quantitative analysis of VH gene usage in adult mice.

Sheehan K, Brodeur P EMBO J. 1989; 8(8):2313-20.

PMID: 2477243 PMC: 401164. DOI: 10.1002/j.1460-2075.1989.tb08358.x.

Biased expression of JH-proximal VH genes occurs in the newly generated repertoire of neonatal and adult mice.

Malynn B, Yancopoulos G, Barth J, Bona C, Alt F J Exp Med. 1990; 171(3):843-59.

PMID: 2261012 PMC: 2187788. DOI: 10.1084/jem.171.3.843.

The physical organization of the human immunoglobulin heavy chain gene complex.

Walter M, Surti U, Hofker M, Cox D EMBO J. 1990; 9(10):3303-13.

PMID: 2170112 PMC: 552067. DOI: 10.1002/j.1460-2075.1990.tb07530.x.

References

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