Central Role of the PPARγ Gene Network in Coordinating Beef Cattle Intramuscular Adipogenesis in Response to Weaning Age and Nutrition

Overview

Journal Gene Regul Syst Bio

Publisher Sage Publications

Date 2014 Feb 12

PMID 24516329

Citations 30

Authors

Sonia J Moisa

Daniel W Shike

Dan B Faulkner

William T Meteer

Duane Keisler

Juan J Loor

Affiliations

Soon will be listed here.

Abstract

Adipogenic/lipogenic transcriptional networks regulating intramuscular fat deposition (IMF) in response to weaning age and dietary starch level were studied. The longissimus muscle (LM) of beef steers on an early weaning (141 days age) plus high-starch diet (EWS) or a normal weaning (NW, 222 days age) plus starch creep-feed diet (CFS) was biopsied at 0 (EW), 25, 50, 96 (NW), 167, and 222 (pre-slaughter) days. Expression patterns of 35 target genes were studied. From NW through slaughter, all steers received the same high-starch diet. In EWS steers the expression of PPARG, other adipogenic (CEBPA, ZFP423) and lipogenic (THRSP, SREBF1, INSIG1) activators, and several enzymes (FASN, SCD, ELOVL6, PCK1, DGAT2) that participate in the process of IMF increased gradually to a peak between 96 and 167 days on treatment. Steers in NW did not achieve similar expression levels even by 222 days on treatment, suggesting a blunted response even when fed a high-starch diet after weaning. High-starch feeding at an early age (EWS) triggers precocious and sustained adipogenesis, resulting in greater marbling.

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References

Sun J, Gao Y, Liu D, Ma W, Xue J, Zhang C . Haplotype combination of the bovine INSIG1 gene sequence variants and association with growth traits in Nanyang cattle. Genome. 2012; 55(6):429-36. DOI: 10.1139/g2012-029. View

Brivanlou A, Darnell Jr J . Signal transduction and the control of gene expression. Science. 2002; 295(5556):813-8. DOI: 10.1126/science.1066355. View

Combs T, Wagner J, Berger J, Doebber T, Wang W, Zhang B . Induction of adipocyte complement-related protein of 30 kilodaltons by PPARgamma agonists: a potential mechanism of insulin sensitization. Endocrinology. 2002; 143(3):998-1007. DOI: 10.1210/endo.143.3.8662. View

Davie J, Chadee D . Regulation and regulatory parameters of histone modifications. J Cell Biochem Suppl. 1999; 30-31:203-13. View

Lozeman F, Middleton C, Deng J, Kazala E, Verhaege C, Mir P . Characterization of microsomal diacylglycerol acyltransferase activity from bovine adipose and muscle tissue. Comp Biochem Physiol B Biochem Mol Biol. 2001; 130(1):105-15. DOI: 10.1016/s1096-4959(01)00413-4. View

Rosen E, Hsu C, Wang X, Sakai S, Freeman M, Gonzalez F . C/EBPalpha induces adipogenesis through PPARgamma: a unified pathway. Genes Dev. 2002; 16(1):22-6. PMC: 155311. DOI: 10.1101/gad.948702. View

Munekata K, Sakamoto K . Forkhead transcription factor Foxo1 is essential for adipocyte differentiation. In Vitro Cell Dev Biol Anim. 2009; 45(10):642-51. DOI: 10.1007/s11626-009-9230-5. View

Govoni K, Hoagland T, Zinn S . The ontogeny of the somatotropic axis in male and female Hereford calves from birth to one year of age. J Anim Sci. 2003; 81(11):2811-7. DOI: 10.2527/2003.81112811x. View

Fan W, Imamura T, Sonoda N, Sears D, Patsouris D, Kim J . FOXO1 transrepresses peroxisome proliferator-activated receptor gamma transactivation, coordinating an insulin-induced feed-forward response in adipocytes. J Biol Chem. 2009; 284(18):12188-97. PMC: 2673287. DOI: 10.1074/jbc.M808915200. View

10.

Moisa S, Shike D, Meteer W, Keisler D, Faulkner D, Loor J . Yin yang 1 and adipogenic gene network expression in longissimus muscle of beef cattle in response to nutritional management. Gene Regul Syst Bio. 2013; 7:71-83. PMC: 3653888. DOI: 10.4137/GRSB.S11783. View

11.

Vincent A, Louveau I, Gondret F, Lebret B, Damon M . Mitochondrial function, fatty acid metabolism, and immune system are relevant features of pig adipose tissue development. Physiol Genomics. 2012; 44(22):1116-24. DOI: 10.1152/physiolgenomics.00098.2012. View

12.

Jeong Y, Kim D, Lee Y, Kim H, Han J, Im S . Integrated expression profiling and genome-wide analysis of ChREBP targets reveals the dual role for ChREBP in glucose-regulated gene expression. PLoS One. 2011; 6(7):e22544. PMC: 3141076. DOI: 10.1371/journal.pone.0022544. View

13.

Beale E, Harvey B, Forest C . PCK1 and PCK2 as candidate diabetes and obesity genes. Cell Biochem Biophys. 2007; 48(2-3):89-95. DOI: 10.1007/s12013-007-0025-6. View

14.

Archibeque S, Lunt D, Gilbert C, Tume R, Smith S . Fatty acid indices of stearoyl-CoA desaturase do not reflect actual stearoyl-CoA desaturase enzyme activities in adipose tissues of beef steers finished with corn-, flaxseed-, or sorghum-based diets. J Anim Sci. 2005; 83(5):1153-66. DOI: 10.2527/2005.8351153x. View

15.

Powell M, Keisler D . A potential strategy for decreasing milk production in the ewe at weaning using a growth hormone release blocker. J Anim Sci. 1995; 73(7):1901-5. DOI: 10.2527/1995.7371901x. View

16.

Bennett M, Seo Y, Datta S, Shin D, Osborne T . Selective binding of sterol regulatory element-binding protein isoforms and co-regulatory proteins to promoters for lipid metabolic genes in liver. J Biol Chem. 2008; 283(23):15628-37. PMC: 2414284. DOI: 10.1074/jbc.M800391200. View

17.

Lin F, Qin J, Tang K, Tsai S, Tsai M . Coup d'Etat: an orphan takes control. Endocr Rev. 2011; 32(3):404-21. PMC: 3365794. DOI: 10.1210/er.2010-0021. View

18.

Bonnet M, Faulconnier Y, Leroux C, Jurie C, Cassar-Malek I, Bauchart D . Glucose-6-phosphate dehydrogenase and leptin are related to marbling differences among Limousin and Angus or Japanese Black x Angus steers. J Anim Sci. 2007; 85(11):2882-94. DOI: 10.2527/jas.2007-0062. View

19.

Hata K, Nishimura R, Ikeda F, Yamashita K, Matsubara T, Nokubi T . Differential roles of Smad1 and p38 kinase in regulation of peroxisome proliferator-activating receptor gamma during bone morphogenetic protein 2-induced adipogenesis. Mol Biol Cell. 2003; 14(2):545-55. PMC: 149991. DOI: 10.1091/mbc.e02-06-0356. View

20.

Meyer D, Kerley M, WALKER E, Keisler D, Pierce V, Schmidt T . Growth rate, body composition, and meat tenderness in early vs. traditionally weaned beef calves. J Anim Sci. 2005; 83(12):2752-61. DOI: 10.2527/2005.83122752x. View