Closed Vitrification of Mouse Oocytes Using the CryoLogic Vitrification Method: A Modification That Improves Developmental Competence

Overview

Journal Clin Exp Reprod Med

Date 2014 Feb 8

PMID 24505560

Citations 1

Authors

Jun Woo Jo

Byung Chul Jee

Chang Suk Suh

Seok Hyun Kim

Affiliations

Soon will be listed here.

Abstract

Objective: To compare the mouse oocyte vitrification outcomes of the CryoLogic vitrification method (CVM) and the conventional open method using a Cryotop. Two CVM methods (original CVM and modified CVM) were tested.

Methods: Mature oocytes obtained from female BDF-1 mice were vitrified by two-step exposure to equilibrium and vitrification solutions. Three vitrification protocols were tested on three groups: the CVM-kit, modified CVM, and Cryotop groups. After exposure to the two solutions, the oocytes were vitrified. After warming, the oocytes were fertilized in vitro, and the embryo development was assessed. Blastomeres positive for caspase were counted using an in situ assay kit. The spindle morphology and chromosome configurations of warmed vitrified oocytes were also assessed.

Results: The modified CVM and Cryotop groups showed similar developmental capacities, and similar proportions of cells with intact spindles and chromosome configurations. The modified CVM protocol was superior to the original CVM protocol for developmental competence and intact spindle preservation. However, the CVM group showed a relatively higher number of apoptotic cells in blastocysts.

Conclusion: Closed vitrification using the modified CVM protocol may be used as an alternative to the conventional open method, but strategies to decrease apoptosis in the blastomere need to be investigated.

Citing Articles

Comparing the effects of a commercial and a prototype vitrification medium on meiotic spindle morphology and survival rate of mouse oocytes.

Viana I, Vireque A, Navarro P JBRA Assist Reprod. 2022; 26(3):500-507.

PMID: 35261222 PMC: 9355432. DOI: 10.5935/1518-0557.20210117.

References

Parmegiani L, Cognigni G, Filicori M . Ultra-violet sterilization of liquid nitrogen prior to vitrification. Hum Reprod. 2009; 24(11):2969. DOI: 10.1093/humrep/dep329. View

Nagy Z, Chang C, Shapiro D, Bernal D, Kort H, Vajta G . The efficacy and safety of human oocyte vitrification. Semin Reprod Med. 2009; 27(6):450-5. DOI: 10.1055/s-0029-1241054. View

Cobo A, Meseguer M, Remohi J, Pellicer A . Use of cryo-banked oocytes in an ovum donation programme: a prospective, randomized, controlled, clinical trial. Hum Reprod. 2010; 25(9):2239-46. DOI: 10.1093/humrep/deq146. View

Huang J, Buckett W, Gilbert L, Tan S, Chian R . Retrieval of immature oocytes followed by in vitro maturation and vitrification: a case report on a new strategy of fertility preservation in women with borderline ovarian malignancy. Gynecol Oncol. 2007; 105(2):542-4. DOI: 10.1016/j.ygyno.2007.01.036. View

Vanderzwalmen P, Zech N, Prapas Y, Panagiotidis Y, Papatheodorou A, Lejeune B . [Closed carrier device: a reality to vitrify oocytes and embryos in aseptic conditions]. Gynecol Obstet Fertil. 2010; 38(9):541-6. DOI: 10.1016/j.gyobfe.2010.07.011. View

Nagy Z, Chang C, Shapiro D, Bernal D, Elsner C, Mitchell-Leef D . Clinical evaluation of the efficiency of an oocyte donation program using egg cryo-banking. Fertil Steril. 2008; 92(2):520-6. DOI: 10.1016/j.fertnstert.2008.06.005. View

Parmegiani L, Accorsi A, Cognigni G, Bernardi S, Troilo E, Filicori M . Sterilization of liquid nitrogen with ultraviolet irradiation for safe vitrification of human oocytes or embryos. Fertil Steril. 2009; 94(4):1525-1528. DOI: 10.1016/j.fertnstert.2009.05.089. View

Ubaldi F, Anniballo R, Romano S, Baroni E, Albricci L, Colamaria S . Cumulative ongoing pregnancy rate achieved with oocyte vitrification and cleavage stage transfer without embryo selection in a standard infertility program. Hum Reprod. 2010; 25(5):1199-205. PMC: 2854047. DOI: 10.1093/humrep/deq046. View

Rienzi L, Romano S, Albricci L, Maggiulli R, Capalbo A, Baroni E . Embryo development of fresh 'versus' vitrified metaphase II oocytes after ICSI: a prospective randomized sibling-oocyte study. Hum Reprod. 2009; 25(1):66-73. PMC: 2794665. DOI: 10.1093/humrep/dep346. View

10.

Kuleshova L, Shaw J . A strategy for rapid cooling of mouse embryos within a double straw to eliminate the risk of contamination during storage in liquid nitrogen. Hum Reprod. 2000; 15(12):2604-9. DOI: 10.1093/humrep/15.12.2604. View

11.

Cremades N, Sousa M, Silva J, Viana P, Sousa S, Oliveira C . Experimental vitrification of human compacted morulae and early blastocysts using fine diameter plastic micropipettes. Hum Reprod. 2004; 19(2):300-5. DOI: 10.1093/humrep/deh059. View

12.

Bielanski A, Nadin-Davis S, Sapp T . Viral contamination of embryos cryopreserved in liquid nitrogen. Cryobiology. 2000; 40(2):110-6. DOI: 10.1006/cryo.1999.2227. View

13.

Smith G, Serafini P, Fioravanti J, Yadid I, Coslovsky M, Hassun P . Prospective randomized comparison of human oocyte cryopreservation with slow-rate freezing or vitrification. Fertil Steril. 2010; 94(6):2088-95. DOI: 10.1016/j.fertnstert.2009.12.065. View

14.

Wang X, Catt S, Pangestu M, Temple-Smith P . Successful in vitro culture of pre-antral follicles derived from vitrified murine ovarian tissue: oocyte maturation, fertilization, and live births. Reproduction. 2010; 141(2):183-91. DOI: 10.1530/REP-10-0383. View

15.

Kuwayama M, Vajta G, Ieda S, Kato O . Comparison of open and closed methods for vitrification of human embryos and the elimination of potential contamination. Reprod Biomed Online. 2006; 11(5):608-14. DOI: 10.1016/s1472-6483(10)61169-8. View

16.

Chian R, Huang J, Tan S, Lucena E, Saa A, Rojas A . Obstetric and perinatal outcome in 200 infants conceived from vitrified oocytes. Reprod Biomed Online. 2008; 16(5):608-10. DOI: 10.1016/s1472-6483(10)60471-3. View

17.

Katayama K, Stehlik J, Kuwayama M, Kato O, Stehlik E . High survival rate of vitrified human oocytes results in clinical pregnancy. Fertil Steril. 2003; 80(1):223-4. DOI: 10.1016/s0015-0282(03)00551-x. View

18.

Beebe L, Bouwman E, McIlfatrick S, Nottle M . Piglets produced from in vivo blastocysts vitrified using the Cryologic Vitrification Method (solid surface vitrification) and a sealed storage container. Theriogenology. 2011; 75(8):1453-8. DOI: 10.1016/j.theriogenology.2010.11.043. View

19.

Isachenko V, Montag M, Isachenko E, Zaeva V, Krivokharchenko I, Shafei R . Aseptic technology of vitrification of human pronuclear oocytes using open-pulled straws. Hum Reprod. 2004; 20(2):492-6. DOI: 10.1093/humrep/deh605. View

20.

Bielanski A, Bergeron H, Lau P, Devenish J . Microbial contamination of embryos and semen during long term banking in liquid nitrogen. Cryobiology. 2003; 46(2):146-52. DOI: 10.1016/s0011-2240(03)00020-8. View