Lysosome Transport As a Function of Lysosome Diameter

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2014 Feb 6

PMID 24497985

Citations 75

Authors

Debjyoti Bandyopadhyay

Austin Cyphersmith

Jairo A Zapata

Y Joseph Kim

Christine K Payne

Affiliations

Soon will be listed here.

Abstract

Lysosomes are membrane-bound organelles responsible for the transport and degradation of intracellular and extracellular cargo. The intracellular motion of lysosomes is both diffusive and active, mediated by motor proteins moving lysosomes along microtubules. We sought to determine how lysosome diameter influences lysosome transport. We used osmotic swelling to double the diameter of lysosomes, creating a population of enlarged lysosomes. This allowed us to directly examine the intracellular transport of the same organelle as a function of diameter. Lysosome transport was measured using live cell fluorescence microscopy and single particle tracking. We find, as expected, the diffusive component of intracellular transport is decreased proportional to the increased lysosome diameter. Active transport of the enlarged lysosomes is not affected by the increased lysosome diameter.

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