Nanographene-Based Polymeric Nanoparticles As Near-Infrared Emissive Neuronal Tracers

Overview

Journal ACS Nano

Publisher American Chemical Society

Specialty Biotechnology

Date 2024 Dec 13

PMID 39668551

Authors

Hao Zhao

Laurent Guillaud

Maria Fransiska Emily

Xiushang Xu

Liliia Moshniaha

Hiroki Hanayama

Ryota Kabe

Marco Terenzio

Akimitsu Narita

Affiliations

Soon will be listed here.

Abstract

Precise tracking of axonal transport is key to deciphering neuronal functions. To achieve long-term imaging at both ultrastructural and macroscopic resolutions, it is critical to develop fluorescent transport tracers with high photostability and biocompatibility. Herein, we report the investigation of nanographene (NG)-based polymeric nanoparticles (NPs) as near-infrared (NIR)-emissive neuronal tracers. Dibenzo[,]dinaphtho[3,2,1-:1',2',3'-]coronene (DBDNC) was employed as the NG, which exhibited a broad NIR emission with a maximum at 711 nm inside the NPs. DBDNC-NPs displayed high photostability and low cytotoxicity, enabling live tracing of retrograde axonal transport in mouse sensory neurons cultured in microfluidic chambers. We also elucidated how DBDNC-NPs undergo retrograde axonal transport following the endolysosomal pathway. This work provides a proof of concept for NIR-emissive, NG-based neuronal tracers with potential for applications in neurobiology.

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