Detection of SLE Antigens in Neutrophil Extracellular Traps (NETs)

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2014 Feb 6

PMID 24497360

Citations 20

Authors

Carmelo Carmona-Rivera

Mariana J Kaplan

Affiliations

Soon will be listed here.

Abstract

Neutrophils are sentinel cells of the innate immune system with a primary role of clearing extracellular pathogens. The release of weblike structures decorated with granular proteins called neutrophil extracellular traps (NETs) has recently been implicated in the pathogenesis of inflammatory and autoimmune diseases. Indeed, NETs may represent an important source of autoantigens and immunostimulatory proteins in systemic lupus erythematosus (SLE). In this chapter, we describe protocols to isolate human peripheral neutrophils, to generate and isolate NETs, and to detect SLE antigens in NETs using immunofluorescence and immunoblot.

Citing Articles

Increased neutrophil extracellular trap formation in oligoarticular, polyarticular juvenile idiopathic arthritis and enthesitis-related arthritis: biomarkers for diagnosis and disease activity.

Tang H, Zhong Y, Wu Y, Huang Y, Liu Y, Chen J Front Immunol. 2024; 15:1436193.

PMID: 39185410 PMC: 11341361. DOI: 10.3389/fimmu.2024.1436193.

Cystic fibrosis autoantibody signatures associate with lung infection or cystic fibrosis-related diabetes.

Yadav R, Li Q, Huang H, Bridges Jr S, Kahlenberg J, Stecenko A Front Immunol. 2023; 14:1151422.

PMID: 37767091 PMC: 10519797. DOI: 10.3389/fimmu.2023.1151422.

Neutrophil extracellular traps involved in the pathogenesis of IgA vasculitis: Confirmed in two IgAV rat models.

Chen X, Zou J, Tu L, Yun X, Qin Y PLoS One. 2023; 18(7):e0288538.

PMID: 37478141 PMC: 10361466. DOI: 10.1371/journal.pone.0288538.

Advances in the Mechanism of Action of Neutrophil Extracellular Traps in Gastrointestinal Tumors: A Review.

Zhang Z, Wang B, Tan B Int J Gen Med. 2023; 16:2783-2789.

PMID: 37408845 PMC: 10319275. DOI: 10.2147/IJGM.S419542.

Apoptosis-induced nuclear expulsion in tumor cells drives S100a4-mediated metastatic outgrowth through the RAGE pathway.

Park W, Gray J, Holewinski R, Andresson T, So J, Carmona-Rivera C Nat Cancer. 2023; 4(3):419-435.

PMID: 36973439 PMC: 10042736. DOI: 10.1038/s43018-023-00524-z.

References

Zhao M, Liu N, Zhang Y, Wang H . Antineutrophil cytoplasmic autoantibodies (ANCA) and their target antigens in Chinese patients with lupus nephritis. Nephrol Dial Transplant. 1998; 13(11):2821-4. DOI: 10.1093/ndt/13.11.2821. View

Monestier M, Decker P, Briand J, Gabriel J, Muller S . Molecular and structural properties of three autoimmune IgG monoclonal antibodies to histone H2B. J Biol Chem. 2000; 275(18):13558-63. DOI: 10.1074/jbc.275.18.13558. View

Papayannopoulos V, Metzler K, Hakkim A, Zychlinsky A . Neutrophil elastase and myeloperoxidase regulate the formation of neutrophil extracellular traps. J Cell Biol. 2010; 191(3):677-91. PMC: 3003309. DOI: 10.1083/jcb.201006052. View

van Bavel C, Dieker J, Kroeze Y, Tamboer W, Voll R, Muller S . Apoptosis-induced histone H3 methylation is targeted by autoantibodies in systemic lupus erythematosus. Ann Rheum Dis. 2010; 70(1):201-7. DOI: 10.1136/ard.2010.129320. View

Villanueva E, Yalavarthi S, Berthier C, Hodgin J, Khandpur R, Lin A . Netting neutrophils induce endothelial damage, infiltrate tissues, and expose immunostimulatory molecules in systemic lupus erythematosus. J Immunol. 2011; 187(1):538-52. PMC: 3119769. DOI: 10.4049/jimmunol.1100450. View

Knight J, Carmona-Rivera C, Kaplan M . Proteins derived from neutrophil extracellular traps may serve as self-antigens and mediate organ damage in autoimmune diseases. Front Immunol. 2012; 3:380. PMC: 3521997. DOI: 10.3389/fimmu.2012.00380. View

Ben Mansour R, Lassoued S, Gargouri B, El Gaid A, Attia H, Fakhfakh F . Increased levels of autoantibodies against catalase and superoxide dismutase associated with oxidative stress in patients with rheumatoid arthritis and systemic lupus erythematosus. Scand J Rheumatol. 2008; 37(2):103-8. DOI: 10.1080/03009740701772465. View

Amulic B, Cazalet C, Hayes G, Metzler K, Zychlinsky A . Neutrophil function: from mechanisms to disease. Annu Rev Immunol. 2012; 30:459-89. DOI: 10.1146/annurev-immunol-020711-074942. View

Borregaard N . Neutrophils, from marrow to microbes. Immunity. 2010; 33(5):657-70. DOI: 10.1016/j.immuni.2010.11.011. View

10.

Kahlenberg J, Carmona-Rivera C, Smith C, Kaplan M . Neutrophil extracellular trap-associated protein activation of the NLRP3 inflammasome is enhanced in lupus macrophages. J Immunol. 2012; 190(3):1217-26. PMC: 3552129. DOI: 10.4049/jimmunol.1202388. View

11.

Cambridge G, Wallace H, Bernstein R, Leaker B . Autoantibodies to myeloperoxidase in idiopathic and drug-induced systemic lupus erythematosus and vasculitis. Br J Rheumatol. 1994; 33(2):109-14. DOI: 10.1093/rheumatology/33.2.109. View

12.

Mosca M, Chimenti D, Pratesi F, Baldini C, Anzilotti C, Bombardieri S . Prevalence and clinico-serological correlations of anti-alpha-enolase, anti-C1q, and anti-dsDNA antibodies in patients with systemic lupus erythematosus. J Rheumatol. 2006; 33(4):695-7. View

13.

Nassberger L, Jonsson H, Sjoholm A, Sturfelt G, Heubner A . Circulating anti-elastase in systemic lupus erythematosus. Lancet. 1989; 1(8636):509. DOI: 10.1016/s0140-6736(89)91420-7. View

14.

Tamiya H, Tani K, Miyata J, Sato K, Urata T, Lkhagvaa B . Defensins- and cathepsin G-ANCA in systemic lupus erythematosus. Rheumatol Int. 2006; 27(2):147-52. DOI: 10.1007/s00296-006-0173-9. View

15.

Robinson W, Digennaro C, Hueber W, Haab B, Kamachi M, Dean E . Autoantigen microarrays for multiplex characterization of autoantibody responses. Nat Med. 2002; 8(3):295-301. DOI: 10.1038/nm0302-295. View

16.

Lande R, Ganguly D, Facchinetti V, Frasca L, Conrad C, Gregorio J . Neutrophils activate plasmacytoid dendritic cells by releasing self-DNA-peptide complexes in systemic lupus erythematosus. Sci Transl Med. 2011; 3(73):73ra19. PMC: 3399524. DOI: 10.1126/scitranslmed.3001180. View

17.

Brinkmann V, Reichard U, Goosmann C, Fauler B, Uhlemann Y, Weiss D . Neutrophil extracellular traps kill bacteria. Science. 2004; 303(5663):1532-5. DOI: 10.1126/science.1092385. View

18.

Nassberger L, Sjoholm A, Jonsson H, Sturfelt G, Akesson A . Autoantibodies against neutrophil cytoplasm components in systemic lupus erythematosus and in hydralazine-induced lupus. Clin Exp Immunol. 1990; 81(3):380-3. PMC: 1534988. DOI: 10.1111/j.1365-2249.1990.tb05342.x. View

19.

Lee S, Lawton J, Chan C, Li C, Kwan T, Chau K . Antilactoferrin antibody in systemic lupus erythematosus. Br J Rheumatol. 1992; 31(10):669-73. DOI: 10.1093/rheumatology/31.10.669. View

20.

Caccavo D, Rigon A, Picardi A, Galluzzo S, Vadacca M, Ferri G . Anti-lactoferrin antibodies in systemic lupus erythematosus: isotypes and clinical correlates. Clin Rheumatol. 2004; 24(4):381-7. DOI: 10.1007/s10067-004-1040-2. View