Thymic Stromal Lmphopoietin Pomotes Macrophage-derived Foam Cell Formation

Overview

Journal J Huazhong Univ Sci Technolog Med Sci

Specialty General Medicine

Date 2014 Feb 6

PMID 24496674

Citations 1

Authors

Da-Zhu Li

Bo-Yuan Wang

Bao-Jie Yang

Shao-Lin He

Jing Lin

Jiang-Chuan Dong

Chun Wu

Jun Hu

Affiliations

Soon will be listed here.

Abstract

The effect of thymic stromal lymphopoietin (TSLP) on macrophage-derived foam cell formation and the underlying mechanism were studied. Macrophages isolated from C57BL/6 mice were co-cultured in vitro with different concentrations of TSLP or TSLPR-antibody in the presence of oxidized low density lipoprotein (ox-LDL). The effects of TSLP on macrophage-derived foam cell formation were observed by using oil red O staining and intracellular lipid determination. The expression levels of foam cell scavenger receptors (CD36 and SRA) as well as ABCA1 and TSLPR were detected by using RT-PCR and Western blotting. As compared with the control group, TSLP treatment significantly promoted lipid accumulation in macrophages, significantly increased protein expression of CD36 and TSLPR in a dose-dependent manner, and significantly reduced the expression of ABCA1 protein in a dose-dependent manner. No significant differences were noted between the TSLPR-antibody group and the control group. TSLP may down-regulate the expression of cholesterol efflux receptor ABCA1 and up-regulate scavenger receptor expression via the TSLPR signaling pathway, thereby promoting macrophage-derived foam cell formation.

Citing Articles

Macrophage Heterogeneity and Plasticity: Impact of Macrophage Biomarkers on Atherosclerosis.

Rojas J, Salazar J, Martinez M, Palmar J, Bautista J, Chavez-Castillo M Scientifica (Cairo). 2015; 2015:851252.

PMID: 26491604 PMC: 4600540. DOI: 10.1155/2015/851252.

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