Human Thymic Stromal Lymphopoietin Enhances Expression of CD80 in Human CD14+ Monocytes/macrophages

Overview

Journal Inflamm Res

Specialties Allergy & Immunology
Pathology

Date 2011 Jan 29

PMID 21274737

Citations 13

Authors

Reiji Hirano

Shunji Hasegawa

Kunio Hashimoto

Yasuhiro Haneda

Ayami Ohsaki

Takashi Ichiyama

Affiliations

Soon will be listed here.

Abstract

Objective: Human thymic stromal lymphopoietin (TSLP), an epithelial cell-derived cytokine, promotes inflammatory T helper type 2 cell (Th2) differentiation of naive CD4(+) T cells. TSLP is highly produced in keratinocytes of patients with atopic dermatitis and bronchial epithelia of patients with asthma and was thought to be a master switch for allergic inflammation. We sought to examine the effect of TSLP in human monocytes/macrophages.

Methods: The effect of TSLP on the expression of cell surface antigens (CD11c, CD16, CD54, CD80, CD86, and HLA-DR) in peripheral blood CD14(+) monocytes/macrophages was examined.

Results: TSLP enhanced the expression of CD80 in peripheral blood CD14(+) monocytes/macrophages but not that of other cell surface antigens. It was associated with an increased percentage of CD14(dim/-), CD80(+), CD11c(+), and HLA-DR(+) cells, which was consistent with the increased differentiation of myeloid dendritic cells.

Conclusions: TSLP induces CD80 expression in human peripheral blood CD14(+) monocytes/macrophages; this indicates monocyte/macrophage activation. This may be associated with their differentiation into myeloid dendritic cells.

Citing Articles

Exploring thymic stromal lymphopoietin in the breast cancer microenvironment: A preliminary study.

Marcella S, Braile M, Grimaldi A, Soricelli A, Smaldone G Oncol Lett. 2025; 29(4):182.

PMID: 40007626 PMC: 11851057. DOI: 10.3892/ol.2025.14928.

Multifactorial Causes and Consequences of TLSP Production, Function, and Release in the Asthmatic Airway.

Brister D, Omer H, Whetstone C, Ranjbar M, Gauvreau G Biomolecules. 2024; 14(4).

PMID: 38672419 PMC: 11048646. DOI: 10.3390/biom14040401.

Severe Asthmatic Responses: The Impact of TSLP.

Theofani E, Tsitsopoulou A, Morianos I, Semitekolou M Int J Mol Sci. 2023; 24(8).

PMID: 37108740 PMC: 10142872. DOI: 10.3390/ijms24087581.

Epithelial immune regulation of inflammatory airway diseases: Chronic rhinosinusitis with nasal polyps (CRSwNP).

Klimek L, Hagemann J, Welkoborsky H, Cuevas M, Casper I, Forster-Ruhrmann U Allergol Select. 2022; 6:148-166.

PMID: 35572064 PMC: 9097524. DOI: 10.5414/ALX02296E.

The Role of Airway Epithelial Cell Alarmins in Asthma.

Whetstone C, Ranjbar M, Omer H, Cusack R, Gauvreau G Cells. 2022; 11(7).

PMID: 35406669 PMC: 8997824. DOI: 10.3390/cells11071105.

References

Soumelis V, Reche P, Kanzler H, Yuan W, Edward G, Homey B . Human epithelial cells trigger dendritic cell mediated allergic inflammation by producing TSLP. Nat Immunol. 2002; 3(7):673-80. DOI: 10.1038/ni805. View

Hashimoto K, Ichiyama T, Hasegawa M, Hasegawa S, Matsubara T, Furukawa S . Cysteinyl leukotrienes induce monocyte chemoattractant protein-1 in human monocyte/macrophages via mitogen-activated protein kinase and nuclear factor-kappaB pathways. Int Arch Allergy Immunol. 2009; 149(3):275-82. DOI: 10.1159/000199724. View

Sun Z, Qiu Y, Shi Y, Tao R, Chen J, Ge Y . Time courses of B7 family molecules expressed on activated T-cells and their biological significance. Cell Immunol. 2005; 236(1-2):146-53. DOI: 10.1016/j.cellimm.2005.08.021. View

Ito T, Wang Y, Duramad O, Hori T, Delespesse G, Watanabe N . TSLP-activated dendritic cells induce an inflammatory T helper type 2 cell response through OX40 ligand. J Exp Med. 2005; 202(9):1213-23. PMC: 2213234. DOI: 10.1084/jem.20051135. View

Viksman M, Bochner B, Peebles R, Schleimer R, Liu M . Expression of activation markers on alveolar macrophages in allergic asthmatics after endobronchial or whole-lung allergen challenge. Clin Immunol. 2002; 104(1):77-85. DOI: 10.1006/clim.2002.5233. View

Ray R, Furlonger C, Williams D, Paige C . Characterization of thymic stromal-derived lymphopoietin (TSLP) in murine B cell development in vitro. Eur J Immunol. 1996; 26(1):10-6. DOI: 10.1002/eji.1830260103. View

Chanez P, Vignola A, Lacoste P, Michel F, Godard P, Bousquet J . Increased expression of adhesion molecules (ICAM-1 and LFA-1) on alveolar macrophages from asthmatic patients. Allergy. 1993; 48(8):576-80. DOI: 10.1111/j.1398-9995.1993.tb00751.x. View

Ichiyama T, Hasegawa M, Ueno Y, Makata H, Matsubara T, Furukawa S . Cysteinyl leukotrienes induce monocyte chemoattractant protein 1 in human monocytes/macrophages. Clin Exp Allergy. 2005; 35(9):1214-9. DOI: 10.1111/j.1365-2222.2005.02323.x. View

Park L, Martin U, Garka K, Gliniak B, Di Santo J, Muller W . Cloning of the murine thymic stromal lymphopoietin (TSLP) receptor: Formation of a functional heteromeric complex requires interleukin 7 receptor. J Exp Med. 2000; 192(5):659-70. PMC: 2193276. DOI: 10.1084/jem.192.5.659. View

10.

Kato A, Favoreto Jr S, Avila P, Schleimer R . TLR3- and Th2 cytokine-dependent production of thymic stromal lymphopoietin in human airway epithelial cells. J Immunol. 2007; 179(2):1080-7. PMC: 2220044. DOI: 10.4049/jimmunol.179.2.1080. View

11.

Reche P, Soumelis V, Gorman D, Clifford T, Liu Mr , Travis M . Human thymic stromal lymphopoietin preferentially stimulates myeloid cells. J Immunol. 2001; 167(1):336-43. DOI: 10.4049/jimmunol.167.1.336. View

12.

Isidoro-Garcia M, Davila-Gonzalez I, Pascual de Pedro M, Sanz-Lozano C, Lorente-Toledano F . Interactions between genes and the environment. Epigenetics in allergy. Allergol Immunopathol (Madr). 2007; 35(6):254-8. DOI: 10.1157/13112992. View

13.

Isaksen D, Baumann H, Trobridge P, Farr A, Levin S, Ziegler S . Requirement for stat5 in thymic stromal lymphopoietin-mediated signal transduction. J Immunol. 1999; 163(11):5971-7. View

14.

Watanabe N, Hanabuchi S, Soumelis V, Yuan W, Ho S, De Waal Malefyt R . Human thymic stromal lymphopoietin promotes dendritic cell-mediated CD4+ T cell homeostatic expansion. Nat Immunol. 2004; 5(4):426-34. DOI: 10.1038/ni1048. View

15.

Leonard W, Shores E, Love P . Role of the common cytokine receptor gamma chain in cytokine signaling and lymphoid development. Immunol Rev. 1995; 148:97-114. DOI: 10.1111/j.1600-065x.1995.tb00095.x. View

16.

Tonnel A, Joseph M, Gosset P, Fournier E, Capron A . Stimulation of alveolar macrophages in asthmatic patients after local provocation test. Lancet. 1983; 1(8339):1406-8. DOI: 10.1016/s0140-6736(83)92356-5. View

17.

Ichiyama T, Hasegawa S, Umeda M, Terai K, Matsubara T, Furukawa S . Pranlukast inhibits NF-kappa B activation in human monocytes/macrophages and T cells. Clin Exp Allergy. 2003; 33(6):802-7. DOI: 10.1046/j.1365-2222.2003.01673.x. View

18.

Akamatsu T, Watanabe N, Kido M, Saga K, Tanaka J, Kuzushima K . Human TSLP directly enhances expansion of CD8+ T cells. Clin Exp Immunol. 2008; 154(1):98-106. PMC: 2561086. DOI: 10.1111/j.1365-2249.2008.03731.x. View

19.

Kapsenberg M . Tweaking of memory T helper 2 cells by TSLP. Immunity. 2006; 24(6):673-675. DOI: 10.1016/j.immuni.2006.06.004. View

20.

Allison J . CD28-B7 interactions in T-cell activation. Curr Opin Immunol. 1994; 6(3):414-9. DOI: 10.1016/0952-7915(94)90120-1. View