Generation and Characterization of Regulatory Dendritic Cells Derived from Murine Induced Pluripotent Stem Cells

Overview

Journal Sci Rep

Specialty Science

Date 2014 Feb 6

PMID 24496181

Citations 11

Authors

Qi Zhang

Masayuki Fujino

Shizue Iwasaki

Hiroshi Hirano

Songjie Cai

Yuya Kitajima

Jinhua Xu

Xiao-Kang Li

Affiliations

Soon will be listed here.

Abstract

Regulatory dendritic cells (DCregs) represent a potential therapeutic tool for assessing a variety of immune overreaction conditions; however, current approaches for generating DCregs for therapeutic purposes are limited. We attempted to generate and characterize DCregs from murine induced pluripotent stem (iPS) cells. The iPS cells co-cultured with OP9 cells displayed mesodermally differentiated flat colonies. GM-CSF drove most of the colonies exhibiting a differentiated morphology. Thereafter, cells became morphologically heterologous under the effects of TGF-β and IL-10. Most of the floating cells developed an irregular shape with areas of protrusion. The generated iPS-DCregs demonstrated high CD11b/c and low CD40, CD80, CD86 and MHC-II expressions with a high antigen uptake ability and poor T-cell stimulatory function. Importantly, iPS-DCregs showed immune responsiveness regulation effects both in vitro and in vivo and the ability to generate regulatory T-cells in vitro. Our result illustrates a feasible approach for generating functional DCregs from murine iPS cells.

Citing Articles

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