The MiR-17/106-p38 Axis is a Key Regulator of the Neurogenic-to-gliogenic Transition in Developing Neural Stem/progenitor Cells

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2014 Jan 30

PMID 24474786

Citations 33

Authors

Hayato Naka-Kaneda

Shiho Nakamura

Mana Igarashi

Hisashi Aoi

Hiroaki Kanki

Jun Tsuyama

Shuichi Tsutsumi

Hiroyuki Aburatani

Takuya Shimazaki

Hideyuki Okano

Affiliations

Soon will be listed here.

Abstract

Neural stem/progenitor cell (NSPC) multipotency is highly regulated so that specific neural networks form during development. NSPCs cannot respond to gliogenic signals without acquiring gliogenic competence and decreasing their neurogenic competence as development proceeds. Coup-tfI and Coup-tfII are triggers of these temporal NSPC competence changes. However, the downstream effectors of Coup-tfs that mediate the neurogenic-to-gliogenic competence transition remain unknown. Here, we identified the microRNA-17/106 (miR-17/106)-p38 axis as a critical regulator of this transition. Overexpression of miR-17 inhibited the acquisition of gliogenic competence and forced stage-progressed NSPCs to regain neurogenic competence without altering the methylation status of a glial gene promoter. We also identified Mapk14 (also known as p38) as a target of miR-17/106 and found that Mapk14 inhibition restored neurogenic competence after the neurogenic phase. These results demonstrate that the miR-17/106-p38 axis is a key regulator of the neurogenic-to-gliogenic NSPC competence transition and that manipulation of this axis permits bidirectional control of NSPC multipotency.

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