Polymorphism of the Merozoite Surface Protein-1 Block 2 Region in Plasmodium Falciparum Isolates from Mauritania

Overview

Journal Malar J

Publisher Biomed Central

Specialty Tropical Medicine

Date 2014 Jan 25

PMID 24456636

Citations 23

Authors

Mohamed Salem O Ahmedou Salem

Magatte Ndiaye

Mohamed Ouldabdallahi

Khadijetou M Lekweiry

Herve Bogreau

Lassana Konate

Babacar Faye

Oumar Gaye

Ousmane Faye

Ali O Mohamed Salem O Boukhary

Affiliations

Soon will be listed here.

Abstract

Background: The genetic diversity of Plasmodium falciparum has been extensively studied in various parts of the world. However, limited data are available from Mauritania. The present study examined and compared the genetic diversity of P. falciparum isolates in Mauritania.

Methods: Plasmodium falciparum isolates blood samples were collected from 113 patients attending health facilities in Nouakchott and Hodh El Gharbi regions. K1, Mad20 and RO33 allelic family of msp-1 gene were determined by nested PCR amplification.

Results: K1 family was the predominant allelic type carried alone or in association with Ro33 and Mad20 types (90%; 102/113). Out of the 113 P. falciparum samples, 93(82.3%) harboured more than one parasite genotype. The overall multiplicity of infection was 3.2 genotypes per infection. There was no significant correlation between multiplicity of infection and age of patients. A significant increase of multiplicity of infection was correlated with parasite densities.

Conclusions: The polymorphism of P. falciparum populations from Mauritania was high. Infection with multiple P. falciparum clones was observed, as well as a high multiplicity of infection reflecting both the high endemicity level and malaria transmission in Mauritania.

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