A PCR Method for Molecular Epidemiology of Plasmodium Falciparum Msp-1

Overview

Journal Tokai J Exp Clin Med

Specialty General Medicine

Date 2000 Jan 6

PMID 10622635

Citations 9

Authors

K Tanabe

N Sakihama

O Kaneko

A Saito-Ito

M Kimura

Affiliations

Soon will be listed here.

Abstract

Merozoite surface protein-1 (MSP-1) of Plasmodium falciparum is a strong malaria vaccine candidate. However, MSP-1 exhibits extensive antigenic polymorphism, an issue which may compromise the development of effective vaccine based on this molecule. Since polymorphic nature of MSP-1 has not been fully understood in endemic areas of malaria, variation of the MSP-1 gene (Msp-1) must be studied in detail in natural parasite populations. Here, a PCR-based method for determination of P. falciparum Msp-1 haplotype is described, which can detect up to 24 different haplotypes per infected person. The method can be applied to various purposes of molecular epidemiology of not only Msp-1 haplotype but the genetic structure of P. falciparum populations.

Citing Articles

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