On the Use of Anti-sense RNA: Down-regulation of MRNA Encoding a Metalloproteinase Inhibitor

In this article we describe methods used for producing anti-sense RNA and the mechanisms by which specific eukaryotic gene functions are inhibited. We also describe some of our studies with plasmid constructs producing anti-sense RNA targeted to a murine metalloproteinase inhibitor and review the evidence for naturally occuring anti-sense transcripts in eukaryotes.