Localization of Serine Racemase and Its Role in the Skin

Overview

Journal J Invest Dermatol

Publisher Elsevier

Specialty Dermatology

Date 2014 Jan 21

PMID 24441099

Citations 10

Authors

Ran Inoue

Yoko Yoshihisa

Yosuke Tojo

Chieko Okamura

Yuzo Yoshida

Jiro Kishimoto

Xinghua Luan

Masahiko Watanabe

Mineyuki Mizuguchi

Yuko Nabeshima

Kenji Hamase

Kenji Matsunaga

Tadamichi Shimizu

Hisashi Mori

Affiliations

Soon will be listed here.

Abstract

D-serine is an endogenous coagonist of the N-methyl-D-aspartate (NMDA)-type glutamate receptor in the central nervous system and its synthesis is catalyzed by serine racemase (SR). Recently, the NMDA receptor has been found to be expressed in keratinocytes (KCs) of the skin and involved in the regulation of KC growth and differentiation. However, the localization and role of SR in the skin remain unknown. Here, using SR-knockout (SR-KO) mice as the control, we demonstrated the localization of the SR protein in the granular and cornified layer of the epidermis of wild-type (WT) mice and its appearance in confluent WT KCs. We also demonstrated the existence of a mechanism for conversion of L-serine to D-serine in epidermal KCs. Furthermore, we found increased expression levels of genes involved in the differentiation of epidermal KCs in adult SR-KO mice, and alterations in the barrier function and ultrastructure of the epidermis in postnatal day 5 SR-KO mice. Our findings suggest that SR in the skin epidermis is involved in the differentiation of epidermal KCs and the formation of the skin barrier.

Citing Articles

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Ni X, Inoue R, Wu Y, Yoshida T, Yaku K, Nakagawa T Front Aging Neurosci. 2023; 15:1211067.

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d-Serine controls epidermal vesicle release via NMDA receptor, allowing tissue migration during the metamorphosis of the chordate .

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Accelerated identification of serine racemase inhibitor from Centella asiatica.

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Gut microbiota-derived D-serine protects against acute kidney injury.

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