The Presence of Free D-serine in Rat Brain

Overview

Journal FEBS Lett

Specialty Biochemistry

Date 1992 Jan 13

PMID 1730289

Citations 105

Authors

A Hashimoto

T Nishikawa

T Hayashi

N Fujii

K Harada

T Oka

K Takahashi

Affiliations

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Abstract

Free amino acid enantiomers in adult rat brain extracts were analyzed as their N,O-pentafluoropropionyl isopropyl derivatives by gas chromatography on a capillary column of Chirasil-L-Val. A peak X, which exhibited the same retention time as the N,O-pentafluoropropionyl isopropyl derivative of authentic D-serine, was detected in the brain extracts. Electron impact and positive chemical ionization mass spectra of the peak X of the brain extracts were identical to those of authentic D-serine. The concentration of free D-serine and the ratio of D-serine/total serine in the brain were estimated to be 0.27 and 0.23 mumol/g of wet weight, respectively. These data provide the first evidence that substantial quantities of free D-serine are present in mammalian brain tissues.

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