Candida Albicans and Candida Parapsilosis Rapidly Up-regulate Galectin-3 Secretion by Human Gingival Epithelial Cells

Overview

Journal Mycopathologia

Specialties Microbiology
Pharmacology

Date 2014 Jan 18

PMID 24436012

Citations 6

Authors

Riyoko Tamai

Yusuke Kiyoura

Affiliations

Soon will be listed here.

Abstract

Galectin-3 is a β-galactoside-binding C-type lectin that plays an important role in innate immunity. The purpose of this study was to determine whether Candida albicans and Candida parapsilosis up-regulate galectin-3 secretion by human gingival epithelial cells and gingival fibroblasts. Ca9-22, a human gingival epithelial cell line, and human gingival fibroblasts were incubated in the presence or absence of C. albicans or C. parapsilosis without serum. Levels of secreted human galectin-3 in culture supernatants were measured by enzyme-linked immunosorbent assay. We also pretreated Ca9-22 cells with cytochalasin D (an actin polymerization inhibitor), ALLN (a calpain inhibitor) and LY294002 [a phosphatidylinositol-3 kinase (PI3K) inhibitor] to determine whether the up-regulation of galectin-3 secretion was mediated by cytoskeletal changes, protease activity, or PI3K signaling. Galectin-3 secretion was significantly and rapidly up-regulated by live C. albicans and C. parapsilosis, as well as heat-killed C. albicans. In addition, cytochalasin D, LY294002 and ALLN did not inhibit the up-regulation in galectin-3 secretion. These results suggest that both live and heat-killed C. albicans and C. parapsilosis may increase the activity of the innate immune system and invasion by other microorganisms via up-regulation of galectin-3 secretion.

Citing Articles

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References

Nickel W . The mystery of nonclassical protein secretion. A current view on cargo proteins and potential export routes. Eur J Biochem. 2003; 270(10):2109-19. DOI: 10.1046/j.1432-1033.2003.03577.x. View

Toth A, Csonka K, Jacobs C, Vagvolgyi C, Nosanchuk J, Netea M . Candida albicans and Candida parapsilosis induce different T-cell responses in human peripheral blood mononuclear cells. J Infect Dis. 2013; 208(4):690-8. PMC: 3719900. DOI: 10.1093/infdis/jit188. View

Partridge E, Le Roy C, Di Guglielmo G, Pawling J, Cheung P, Granovsky M . Regulation of cytokine receptors by Golgi N-glycan processing and endocytosis. Science. 2004; 306(5693):120-4. DOI: 10.1126/science.1102109. View

Bertocchi A, Campanhole G, Wang P, Goncalves G, Damiao M, Cenedeze M . A Role for galectin-3 in renal tissue damage triggered by ischemia and reperfusion injury. Transpl Int. 2008; 21(10):999-1007. DOI: 10.1111/j.1432-2277.2008.00705.x. View

Funayama M, Nishikawa A, Shinoda T, Suzuki M, Fukazawa Y . Antigenic relationship between Candida parapsilosis and Candida albicans serotype B. Microbiol Immunol. 1984; 28(12):1359-71. DOI: 10.1111/j.1348-0421.1984.tb00794.x. View

Quattroni P, Li Y, Lucchesi D, Lucas S, Hood D, Herrmann M . Galectin-3 binds Neisseria meningitidis and increases interaction with phagocytic cells. Cell Microbiol. 2012; 14(11):1657-75. PMC: 3749814. DOI: 10.1111/j.1462-5822.2012.01838.x. View

Kohatsu L, Hsu D, Jegalian A, Liu F, Baum L . Galectin-3 induces death of Candida species expressing specific beta-1,2-linked mannans. J Immunol. 2006; 177(7):4718-26. DOI: 10.4049/jimmunol.177.7.4718. View

Dumic J, Lauc G, Flogel M . Expression of galectin-3 in cells exposed to stress-roles of jun and NF-kappaB. Cell Physiol Biochem. 2000; 10(3):149-58. DOI: 10.1159/000016345. View

Saravanan C, Liu F, Gipson I, Panjwani N . Galectin-3 promotes lamellipodia formation in epithelial cells by interacting with complex N-glycans on alpha3beta1 integrin. J Cell Sci. 2009; 122(Pt 20):3684-93. PMC: 2758802. DOI: 10.1242/jcs.045674. View

10.

Tamai R, Sugamata M, Kiyoura Y . Amphotericin B up-regulates lipid A-induced IL-6 production via caspase-8. J Dent Res. 2012; 91(7):709-14. DOI: 10.1177/0022034512446486. View

11.

Samaranayake L, Lamb A, Lamey P, MacFarlane T . Oral carriage of Candida species and coliforms in patients with burning mouth syndrome. J Oral Pathol Med. 1989; 18(4):233-5. DOI: 10.1111/j.1600-0714.1989.tb00769.x. View

12.

Lagana A, Goetz J, Cheung P, Raz A, Dennis J, Nabi I . Galectin binding to Mgat5-modified N-glycans regulates fibronectin matrix remodeling in tumor cells. Mol Cell Biol. 2006; 26(8):3181-93. PMC: 1446937. DOI: 10.1128/MCB.26.8.3181-3193.2006. View

13.

Larsen L, Chen H, Saegusa J, Liu F . Galectin-3 and the skin. J Dermatol Sci. 2011; 64(2):85-91. PMC: 3192432. DOI: 10.1016/j.jdermsci.2011.07.008. View

14.

Gantner B, Simmons R, Canavera S, Akira S, Underhill D . Collaborative induction of inflammatory responses by dectin-1 and Toll-like receptor 2. J Exp Med. 2003; 197(9):1107-17. PMC: 2193968. DOI: 10.1084/jem.20021787. View

15.

Shi J, Zeng X, Zhou M, Chen Q . Activation of ERK-FAK signaling pathway and enhancement of cell migration involved in the early interaction between oral keratinocytes and Candida albicans. Mycopathologia. 2008; 167(1):1-7. DOI: 10.1007/s11046-008-9142-z. View

16.

Menon S, Kang C, Beningo K . Galectin-3 secretion and tyrosine phosphorylation is dependent on the calpain small subunit, Calpain 4. Biochem Biophys Res Commun. 2011; 410(1):91-6. PMC: 3124573. DOI: 10.1016/j.bbrc.2011.05.112. View

17.

Schaller M, Schafer W, Korting H, Hube B . Differential expression of secreted aspartyl proteinases in a model of human oral candidosis and in patient samples from the oral cavity. Mol Microbiol. 1998; 29(2):605-15. DOI: 10.1046/j.1365-2958.1998.00957.x. View

18.

Stillman B, Hsu D, Pang M, Brewer C, Johnson P, Liu F . Galectin-3 and galectin-1 bind distinct cell surface glycoprotein receptors to induce T cell death. J Immunol. 2006; 176(2):778-89. DOI: 10.4049/jimmunol.176.2.778. View

19.

Wellington M, Dolan K, Krysan D . Live Candida albicans suppresses production of reactive oxygen species in phagocytes. Infect Immun. 2008; 77(1):405-13. PMC: 2612242. DOI: 10.1128/IAI.00860-08. View

20.

Baptiste T, James A, Saria M, Ochieng J . Mechano-transduction mediated secretion and uptake of galectin-3 in breast carcinoma cells: implications in the extracellular functions of the lectin. Exp Cell Res. 2006; 313(4):652-64. PMC: 1885467. DOI: 10.1016/j.yexcr.2006.11.005. View