Activation of ERK-FAK Signaling Pathway and Enhancement of Cell Migration Involved in the Early Interaction Between Oral Keratinocytes and Candida Albicans

Overview

Journal Mycopathologia

Specialties Microbiology
Pharmacology

Date 2008 Jul 10

PMID 18612845

Citations 7

Authors

Jing Shi

Xin Zeng

Min Zhou

Qianming Chen

Affiliations

Soon will be listed here.

Abstract

Purposes: To investigate the early molecular events in oral keratinocytes induced by Candida albicans challenge.

Methods: The oral keratinocyte cell line, Tca8113, was used to study the molecular events induced by C. albicans challenge in oral keratinocytes. The phosphorylation statuses of extracellular signal-regulated protein kinase (ERK) and focal adhesion kinase (FAK) upon C. albicans challenge were assessed using specific antibodies and western blotting. Specific inhibitors for ERK and FAK were used to validate the involvement of ERK-FAK signaling cascade. A Transwell insert system-based migration study was performed to evaluate the involvement of the C. albicans-dependent ERK-FAK activation with cell migration.

Results: Following the stimulation with C. albicans, a transient activation of ERK was observed, which reached a peak at 10 min post stimulation. Similarly, a transient activation of FAK, the downstream substrate of ERK, was also observed upon C. albicans challenges, which reach the maximum at 20 min. Specific inhibitors for ERK and FAK abolished the C. albicans-induced ERK and FAK activations. The elevated migratory ability of oral keratinocyte was observed upon stimulation with C. albicans, and was synchronous with the ERK-FAK activation.

Conclusion: ERK-FAK signaling cascades are involved in the early interaction between the oral keratinocytes and C. albicans, which appears to be linked with the enhanced cell migration.

Citing Articles

Oral Microbiota-Driven Cell Migration in Carcinogenesis and Metastasis.

Bai H, Yang J, Meng S, Liu C Front Cell Infect Microbiol. 2022; 12:864479.

PMID: 35573798 PMC: 9103474. DOI: 10.3389/fcimb.2022.864479.

Extract Activates Growth and Survival Signal Transduction Networks to Stimulate Proliferation of Human Keratinocyte.

Nimlamool W, Potikanond S, Ruttanapattanakul J, Wikan N, Okonogi S, Jantrapirom S Biology (Basel). 2021; 10(4).

PMID: 33916174 PMC: 8067174. DOI: 10.3390/biology10040289.

Antifungal Effect of Long Noncoding RNA 9708-1 in the Vulvovaginal Candidiasis Murine Model.

Wu Y, Jiang L, Zhang L, Liu X, Yan L, Luan T Mycopathologia. 2021; 186(2):177-188.

PMID: 33587236 PMC: 8106589. DOI: 10.1007/s11046-021-00530-8.

Peel Extract Suppresses Cell Proliferation and Promotes the Differentiation of Keratinocytes through Inhibition of the EGFR-ERK Signaling Pathway.

Abe S, Ueno M, Nishitani M, Akamatsu T, Sato T, Shimoda M Biomolecules. 2020; 10(10).

PMID: 33096942 PMC: 7589747. DOI: 10.3390/biom10101468.

Novel naphthochalcone derivative accelerate dermal wound healing through induction of epithelial-mesenchymal transition of keratinocyte.

Seo G, Ho M, Bui N, Kim Y, Koh D, Lim Y J Biomed Sci. 2015; 22:47.

PMID: 26130135 PMC: 4488135. DOI: 10.1186/s12929-015-0141-3.

References

Bosio C, Goodyear A, Dow S . Early interaction of Yersinia pestis with APCs in the lung. J Immunol. 2005; 175(10):6750-6. DOI: 10.4049/jimmunol.175.10.6750. View

Zhong B, Jiang K, Gilvary D, Epling-Burnette P, Ritchey C, Liu J . Human neutrophils utilize a Rac/Cdc42-dependent MAPK pathway to direct intracellular granule mobilization toward ingested microbial pathogens. Blood. 2003; 101(8):3240-8. DOI: 10.1182/blood-2001-12-0180. View

Johnson G, Lapadat R . Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases. Science. 2002; 298(5600):1911-2. DOI: 10.1126/science.1072682. View

Skinner J, Pilione M, Shen H, Harvill E, Yuk M . Bordetella type III secretion modulates dendritic cell migration resulting in immunosuppression and bacterial persistence. J Immunol. 2005; 175(7):4647-52. DOI: 10.4049/jimmunol.175.7.4647. View

Ridley A, Schwartz M, Burridge K, Firtel R, Ginsberg M, Borisy G . Cell migration: integrating signals from front to back. Science. 2003; 302(5651):1704-9. DOI: 10.1126/science.1092053. View

Ato M, Maroof A, Zubairi S, Nakano H, Kakiuchi T, Kaye P . Loss of dendritic cell migration and impaired resistance to Leishmania donovani infection in mice deficient in CCL19 and CCL21. J Immunol. 2006; 176(9):5486-93. PMC: 3602896. DOI: 10.4049/jimmunol.176.9.5486. View

Sandovsky-Losica H, Berdicevsky I, Tsarfaty I, Segal E . Effect of Candida albicans metabolite(s) on cellular actin. FEMS Microbiol Lett. 2002; 215(1):57-62. DOI: 10.1111/j.1574-6968.2002.tb11370.x. View

Samaranayake Y, Dassanayake R, Cheung B, Jayatilake J, Yeung K, Yau J . Differential phospholipase gene expression by Candida albicans in artificial media and cultured human oral epithelium. APMIS. 2007; 114(12):857-66. DOI: 10.1111/j.1600-0463.2006.apm_479.x. View

Lu F, Jacobson R . Oral mucosal immunity and HIV/SIV infection. J Dent Res. 2007; 86(3):216-26. DOI: 10.1177/154405910708600305. View

10.

Velan B, Bar-Haim E, Zauberman A, Mamroud E, Shafferman A, Cohen S . Discordance in the effects of Yersinia pestis on the dendritic cell functions manifested by induction of maturation and paralysis of migration. Infect Immun. 2006; 74(11):6365-76. PMC: 1695518. DOI: 10.1128/IAI.00974-06. View

11.

Hunger-Glaser I, Salazar E, Sinnett-Smith J, Rozengurt E . Bombesin, lysophosphatidic acid, and epidermal growth factor rapidly stimulate focal adhesion kinase phosphorylation at Ser-910: requirement for ERK activation. J Biol Chem. 2003; 278(25):22631-43. DOI: 10.1074/jbc.M210876200. View

12.

Pivarcsi A, Kemeny L, Dobozy A . Innate immune functions of the keratinocytes. A review. Acta Microbiol Immunol Hung. 2004; 51(3):303-10. DOI: 10.1556/AMicr.51.2004.3.8. View

13.

Cuevas B, Abell A, Witowsky J, Yujiri T, Johnson N, Kesavan K . MEKK1 regulates calpain-dependent proteolysis of focal adhesion proteins for rear-end detachment of migrating fibroblasts. EMBO J. 2003; 22(13):3346-55. PMC: 165646. DOI: 10.1093/emboj/cdg322. View

14.

Li S, Guan J, Chien S . Biochemistry and biomechanics of cell motility. Annu Rev Biomed Eng. 2005; 7:105-50. DOI: 10.1146/annurev.bioeng.7.060804.100340. View

15.

Hii C, Stacey K, Moghaddami N, Murray A, Ferrante A . Role of the extracellular signal-regulated protein kinase cascade in human neutrophil killing of Staphylococcus aureus and Candida albicans and in migration. Infect Immun. 1999; 67(3):1297-302. PMC: 96460. DOI: 10.1128/IAI.67.3.1297-1302.1999. View

16.

Lilly E, Leigh J, Joseph S, Fidel Jr P . Candida-induced oral epithelial cell responses. Mycopathologia. 2006; 162(1):25-32. DOI: 10.1007/s11046-006-0036-7. View

17.

Sun B, Nishihira J, Yoshiki T, Kondo M, Sato Y, Sasaki F . Macrophage migration inhibitory factor promotes tumor invasion and metastasis via the Rho-dependent pathway. Clin Cancer Res. 2005; 11(3):1050-8. View

18.

Borregaard N, Theilgaard-Monch K, Cowland J, Stahle M, Sorensen O . Neutrophils and keratinocytes in innate immunity--cooperative actions to provide antimicrobial defense at the right time and place. J Leukoc Biol. 2004; 77(4):439-43. DOI: 10.1189/jlb.0704381. View

19.

Chang F, Steelman L, Shelton J, Lee J, Navolanic P, Blalock W . Regulation of cell cycle progression and apoptosis by the Ras/Raf/MEK/ERK pathway (Review). Int J Oncol. 2003; 22(3):469-80. View