Carbon Metabolism of Chloroplasts in the Dark: Oxidative Pentose Phosphate Cycle Versus Glycolytic Pathway

Overview

Journal Planta

Specialty Biology

Date 2014 Jan 11

PMID 24408693

Citations 7

Authors

W M Kaiser

J A Bassham

Affiliations

Soon will be listed here.

Abstract

The conversion of U-labelled [(14)C]glucose-6-phosphate into other products by a soluble fraction of lysed spinach chloroplasts has been studied. It was found that both an oxidative pentose phosphate cycle and a glycolytic reaction sequence occur in this fraction. The formation of bisphosphates and of triose phosphates was ATP-dependent and occurred mainly via a glycolytic reaction sequence including a phosphofructokinase step. The conversion, of glucose-6-phosphate via the oxidative pentose phosphate cycle stopped with the formation of pentose monophosphates. This was found not to be because of a lack in transaldolase (or transketolase) activity, but because of the high concentration ratios of hexose monophosphate/pentose monophosphate used in our experiments for simulating the conditions in whole chloroplasts in the dark. Some regulatory properties of both the oxidative pentose phosphate cycle and of the glycolytic pathway were studied.

Citing Articles

Adjustment of light-responsive NADP dynamics in chloroplasts by stromal pH.

Fukuda Y, Ishiyama C, Kawai-Yamada M, Hashida S Nat Commun. 2023; 14(1):7148.

PMID: 37932304 PMC: 10628217. DOI: 10.1038/s41467-023-42995-9.

Increased Catalase Activity and Maintenance of Photosystem II Distinguishes High-Yield Mutants From Low-Yield Mutants of Rice var. Nagina22 Under Low-Phosphorus Stress.

Poli Y, Nallamothu V, Balakrishnan D, Ramesh P, Desiraju S, Mangrauthia S Front Plant Sci. 2018; 9:1543.

PMID: 30510556 PMC: 6252357. DOI: 10.3389/fpls.2018.01543.

Reversible inhibition of the calvin cycle and activation of oxidative pentose phosphate cycle in isolated intact chloroplasts by hydrogen peroxide.

Kaiser W Planta. 2013; 145(4):377-82.

PMID: 24317766 DOI: 10.1007/BF00388364.

Fatty acid synthesis by isolated chromoplasts from the daffodil. Energy sources and distribution patterns of the acids.

Kleinig H, Liedvogel B Planta. 2013; 150(2):166-9.

PMID: 24306592 DOI: 10.1007/BF00582361.

Photosynthesis under osmotic stress : Effect of high solute concentrations on the permeability properties of the chloroplast envelope and on activity of stroma enzymes.

Kaiser W, Heber U Planta. 2013; 153(5):423-9.

PMID: 24275811 DOI: 10.1007/BF00394980.

References

Steup M, Peavey D, Gibbs M . The regulation of starch metabolism by inorganic phosphate. Biochem Biophys Res Commun. 1976; 72(4):1554-61. DOI: 10.1016/s0006-291x(76)80191-x. View

Bassham J, Krause G . Free energy changes and metabolic regulation in steady-state photosynthetic carbon reduction. Biochim Biophys Acta. 1969; 189(2):207-21. DOI: 10.1016/0005-2728(69)90048-6. View

Lendzian K, Ziegler H . [Regulation of glucose-6-phosphate dehydrogenase in spinach chloroplasts by light]. Planta. 2014; 94(1):27-36. DOI: 10.1007/BF00386606. View

Kelly G, Latzko E . Chloroplast Phosphofructokinase: II. Partial Purification, Kinetic and Regulatory Properties. Plant Physiol. 1977; 60(2):295-9. PMC: 542599. DOI: 10.1104/pp.60.2.295. View

Heldt H, Chon C, Maronde D . Role of orthophosphate and other factors in the regulation of starch formation in leaves and isolated chloroplasts. Plant Physiol. 1977; 59(6):1146-55. PMC: 542524. DOI: 10.1104/pp.59.6.1146. View

Lendzian K, Bassham J . NADPH/NADP+ ratios in photosynthesizing reconstituted chloroplasts. Biochim Biophys Acta. 1976; 430(3):478-89. DOI: 10.1016/0005-2728(76)90024-4. View

Arnon D . COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949; 24(1):1-15. PMC: 437905. DOI: 10.1104/pp.24.1.1. View

Krause G, Bassham J . Induction of respiratory metabolism in illuminated Chlorella pyrenoidosa and isolated spinach chloroplasts by the addition of vitamin K5. Biochim Biophys Acta. 1969; 172(3):553-65. DOI: 10.1016/0005-2728(69)90151-0. View

Lilley R, Chon C, Mosbach A, Heldt H . The distribution of metabolites between spinach chloroplasts and medium during photosynthesis in vitro. Biochim Biophys Acta. 1977; 460(2):259-72. DOI: 10.1016/0005-2728(77)90212-2. View

10.

Kaiser W, Bassham J . Light-Dark Regulation of Starch Metabolism in Chloroplasts: I. Levels of Metabolites in Chloroplasts and Medium during Light-Dark Transition. Plant Physiol. 1979; 63(1):105-8. PMC: 542774. DOI: 10.1104/pp.63.1.105. View

11.

Anderson L, Ng T, Park K . Inactivation of pea leaf chloroplastic and cytoplasmic glucose 6-phosphate dehydrogenases by light and dithiothreitol. Plant Physiol. 1974; 53(6):835-9. PMC: 541458. DOI: 10.1104/pp.53.6.835. View

12.

Jensen R, Bassham J . Photosynthesis by isolated chloroplasts. Proc Natl Acad Sci U S A. 1966; 56(4):1095-101. PMC: 220006. DOI: 10.1073/pnas.56.4.1095. View

13.

Kelly G, Latzko E . Chloroplast phosphofructokinase: I. Proof of phosphofructokinase activity in chloroplasts. Plant Physiol. 1977; 60(2):290-4. PMC: 542598. DOI: 10.1104/pp.60.2.290. View

14.

Lendzian K, Bassham J . Regulation of glucose-6-phosphate dehydrogenase in spinach chloroplasts by ribulose 1,5-diphosphate and NADPH/NADP+ ratios. Biochim Biophys Acta. 1975; 396(2):260-75. DOI: 10.1016/0005-2728(75)90040-7. View

15.

Peavey D, Steup M, Gibbs M . Characterization of starch breakdown in the intact spinach chloroplast. Plant Physiol. 1977; 60(2):305-8. PMC: 542601. DOI: 10.1104/pp.60.2.305. View

16.

Lendzian K . Interactions between magnesium ions, pH, glucose-6-phosphate, and NADPH/NADP(+) ratios in the modulation of chloroplast glucose-6-phosphate dehydrogenase in vitro. Planta. 2014; 141(1):105-10. DOI: 10.1007/BF00387751. View

17.

Chu D, Bassham J . Inhibition of ribulose 1,5-diphosphate carboxylase by 6-phosphogluconate. Plant Physiol. 1972; 50(2):224-7. PMC: 366114. DOI: 10.1104/pp.50.2.224. View