Effects of Acute Stress on Cardiac Endocannabinoids, Lipogenesis, and Inflammation in Rats

Overview

Journal Psychosom Med

Specialty Psychiatry

Date 2013 Dec 25

PMID 24367128

Citations 8

Authors

E Alison Holman

Ana Guijarro

James Lim

Daniele Piomelli

Affiliations

Soon will be listed here.

Abstract

Objective: Trauma exposure can precipitate acute stress (AS) and cardiovascular disorders (CVD). Identifying AS-related physiologic changes that affect CVD risk could inform development of early CVD prevention strategies. The endocannabinoid system (ECS) regulates hypothalamic-pituitary-adrenal axis and stress-related cardiovascular function. We examine stress-related ECS activity and its association with cardiovascular biochemistry/function after AS.

Methods: Rodents (n = 8-16/group) were exposed to predator odor or saline; elevated plus maze, blood pressure, serum and cardiac ECS markers, and lipid metabolism were assessed 24 hours and 2 weeks postexposure.

Results: At 24 hours, the predator odor group demonstrated anxiety-like behavior and had a) elevated serum markers of cardiac failure/damage (brain natriuretic peptide: 275.1 versus 234.6, p = .007; troponin I: 1.50 versus 0.78, p = .076), lipogenesis (triacylglycerols: 123.5 versus 85.93, p = .018), and inflammation (stearoyl delta-9 desaturase activity: 0.21 versus 0.07, p < .001); b) decreased cardiac 2-arachidonoyl-sn-glycerol (29.90 versus 65.95, p < .001), oleoylethanolamide (114.3 versus 125.4, p = .047), and palmitoylethanolamide (72.96 versus 82.87, p = .008); and c) increased cardiac inflammation (interleukin [IL]-1β/IL-6 ratio: 19.79 versus 13.57, p = .038; tumor necrosis factor α/IL-6 ratio: 1.73 versus 1.03, p = .019) and oxidative stress (thiobarbituric acid reactive substances: 7.81 versus 7.05, p = .022), which were associated with cardiac steatosis (higher triacylglycerol: 1.09 versus 0.72, p < .001). Cardiac lipogenesis persisted, and elevated blood pressure emerged 2 weeks postexposure.

Conclusions: Acute psychological stress elicits ECS-related cardiac responses associated with persistent, potentially pathological changes in rat cardiovascular biochemistry/function.

Citing Articles

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References

Wittstein I . Stress cardiomyopathy: a syndrome of catecholamine-mediated myocardial stunning?. Cell Mol Neurobiol. 2012; 32(5):847-57. PMC: 11498407. DOI: 10.1007/s10571-012-9804-8. View

Vassiliadis E, Barascuk N, Didangelos A, Karsdal M . Novel cardiac-specific biomarkers and the cardiovascular continuum. Biomark Insights. 2012; 7:45-57. PMC: 3347891. DOI: 10.4137/BMI.S9536. View

Chen A, Chen D, Daiber A, Faraci F, Li H, Rembold C . Free radical biology of the cardiovascular system. Clin Sci (Lond). 2012; 123(2):73-91. DOI: 10.1042/CS20110562. View

Hebb A, Zacharko R, Gauthier M, Drolet G . Exposure of mice to a predator odor increases acoustic startle but does not disrupt the rewarding properties of VTA intracranial self-stimulation. Brain Res. 2003; 982(2):195-210. DOI: 10.1016/s0006-8993(03)03008-7. View

DiPatrizio N, Piomelli D . The thrifty lipids: endocannabinoids and the neural control of energy conservation. Trends Neurosci. 2012; 35(7):403-11. PMC: 3744874. DOI: 10.1016/j.tins.2012.04.006. View

Peckett A, Wright D, Riddell M . The effects of glucocorticoids on adipose tissue lipid metabolism. Metabolism. 2011; 60(11):1500-10. DOI: 10.1016/j.metabol.2011.06.012. View

Stoney C, Bausserman L, Niaura R, Marcus B, Flynn M . Lipid reactivity to stress: II. Biological and behavioral influences. Health Psychol. 1999; 18(3):251-61. DOI: 10.1037//0278-6133.18.3.251. View

Sharma S, Adrogue J, Golfman L, Uray I, Lemm J, Youker K . Intramyocardial lipid accumulation in the failing human heart resembles the lipotoxic rat heart. FASEB J. 2004; 18(14):1692-700. DOI: 10.1096/fj.04-2263com. View

de Cavanagh E, Inserra F, Ferder L . Angiotensin II blockade: a strategy to slow ageing by protecting mitochondria?. Cardiovasc Res. 2010; 89(1):31-40. DOI: 10.1093/cvr/cvq285. View

10.

Talayero B, Sacks F . The role of triglycerides in atherosclerosis. Curr Cardiol Rep. 2011; 13(6):544-52. PMC: 3234107. DOI: 10.1007/s11886-011-0220-3. View

11.

Lingvay I, Raskin P, Szczepaniak L . The fatty hearts of patients with diabetes. Nat Rev Cardiol. 2009; 6(4):268-9. DOI: 10.1038/nrcardio.2009.30. View

12.

Hill M, Tasker J . Endocannabinoid signaling, glucocorticoid-mediated negative feedback, and regulation of the hypothalamic-pituitary-adrenal axis. Neuroscience. 2012; 204:5-16. PMC: 3288468. DOI: 10.1016/j.neuroscience.2011.12.030. View

13.

Cohen H, Kozlovsky N, Alona C, Matar M, Joseph Z . Animal model for PTSD: from clinical concept to translational research. Neuropharmacology. 2011; 62(2):715-24. DOI: 10.1016/j.neuropharm.2011.04.023. View

14.

Steptoe A, Kivimaki M . Stress and cardiovascular disease. Nat Rev Cardiol. 2012; 9(6):360-70. DOI: 10.1038/nrcardio.2012.45. View

15.

Holman E, Silver R, Poulin M, Andersen J, Gil-Rivas V, McIntosh D . Terrorism, acute stress, and cardiovascular health: a 3-year national study following the September 11th attacks. Arch Gen Psychiatry. 2008; 65(1):73-80. DOI: 10.1001/archgenpsychiatry.2007.6. View

16.

Adamec R, Holmes A, Blundell J . Vulnerability to lasting anxiogenic effects of brief exposure to predator stimuli: sex, serotonin and other factors-relevance to PTSD. Neurosci Biobehav Rev. 2008; 32(7):1287-92. PMC: 2574013. DOI: 10.1016/j.neubiorev.2008.05.005. View

17.

Hongo M, Ishizaka N, Furuta K, Yahagi N, Saito K, Sakurai R . Administration of angiotensin II, but not catecholamines, induces accumulation of lipids in the rat heart. Eur J Pharmacol. 2008; 604(1-3):87-92. DOI: 10.1016/j.ejphar.2008.12.006. View

18.

Solorzano C, Zhu C, Battista N, Astarita G, Lodola A, Rivara S . Selective N-acylethanolamine-hydrolyzing acid amidase inhibition reveals a key role for endogenous palmitoylethanolamide in inflammation. Proc Natl Acad Sci U S A. 2009; 106(49):20966-71. PMC: 2791595. DOI: 10.1073/pnas.0907417106. View

19.

Chida Y, Steptoe A . Greater cardiovascular responses to laboratory mental stress are associated with poor subsequent cardiovascular risk status: a meta-analysis of prospective evidence. Hypertension. 2010; 55(4):1026-32. DOI: 10.1161/HYPERTENSIONAHA.109.146621. View

20.

Saavedra J, Sanchez-Lemus E, Benicky J . Blockade of brain angiotensin II AT1 receptors ameliorates stress, anxiety, brain inflammation and ischemia: Therapeutic implications. Psychoneuroendocrinology. 2010; 36(1):1-18. PMC: 2998923. DOI: 10.1016/j.psyneuen.2010.10.001. View