Rab11 Facilitates Cross-talk Between Autophagy and Endosomal Pathway Through Regulation of Hook Localization

Overview

Journal Mol Biol Cell

Specialties Cell Biology
Molecular Biology

Date 2013 Dec 21

PMID 24356450

Citations 64

Authors

Zsuzsanna Szatmari

Viktor Kis

Monika Lippai

Krisztina Hegedus

Tamas Farago

Peter Lorincz

Tsubasa Tanaka

Gabor Juhasz

Miklos Sass

Affiliations

Soon will be listed here.

Abstract

During autophagy, double-membrane autophagosomes deliver sequestered cytoplasmic content to late endosomes and lysosomes for degradation. The molecular mechanism of autophagosome maturation is still poorly characterized. The small GTPase Rab11 regulates endosomal traffic and is thought to function at the level of recycling endosomes. We show that loss of Rab11 leads to accumulation of autophagosomes and late endosomes in Drosophila melanogaster. Rab11 translocates from recycling endosomes to autophagosomes in response to autophagy induction and physically interacts with Hook, a negative regulator of endosome maturation. Hook anchors endosomes to microtubules, and we show that Rab11 facilitates the fusion of endosomes and autophagosomes by removing Hook from mature late endosomes and inhibiting its homodimerization. Thus induction of autophagy appears to promote autophagic flux by increased convergence with the endosomal pathway.

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