Visual Detection of MicroRNA with Lateral Flow Nucleic Acid Biosensor

Overview

Journal Biosens Bioelectron

Specialties Biochemistry
Biotechnology

Date 2013 Dec 17

PMID 24333569

Citations 34

Authors

Xuefei Gao

Hui Xu

Meenu Baloda

Anant S Gurung

Li-Ping Xu

Tao Wang

Xueji Zhang

Guodong Liu

Affiliations

Soon will be listed here.

Abstract

We report a DNA-gold nanoparticle (DNA-GNP) based lateral flow nucleic acid biosensor for visual detection of microRNA (miRNA)-215 in aqueous solutions and biological samples with low-cost and short analysis time. Sandwich-type hybridization reactions among GNP-labeled DNA probe, miRNA-215 and biotin-modified DNA probes were performed on the lateral flow device. The accumulation of GNPs on the test zone of the biosensor enables the visual detection of miRNA-215. After systematic optimization, the biosensor was able to detect a minimum concentration of 60 pM miRNA-215. The biosensor was applied to detect miRNA-215 from A549 cell lysate directly without complex sample treatment, and the detection limit of 0.148 million cells was obtained. This study provides a simple, rapid, specific and low-cost approach for miRNA detection in aqueous solutions and biological samples, showing great promise for clinical application and biomedical diagnosis in some malignant diseases.

Citing Articles

Rapid Detection of microRNA-122 in Serum and Finger Blood Using a Lateral Flow Nucleic Acid Biosensor.

Zhang M, Ma M, Wang J, Zhou Y, Zhang X, Liu G Biosensors (Basel). 2025; 15(1).

PMID: 39852109 PMC: 11764104. DOI: 10.3390/bios15010058.

Visual detection of microRNAs using gold nanorod-based lateral flow nucleic acid biosensor and exonuclease III-assisted signal amplification.

Zhang J, Yan C, Liu G Mikrochim Acta. 2024; 191(8):491.

PMID: 39066913 DOI: 10.1007/s00604-024-06557-0.

PCR Independent Strategy-Based Biosensors for RNA Detection.

Li X, Wang H, Qi X, Ji Y, Li F, Chen X Biosensors (Basel). 2024; 14(4).

PMID: 38667193 PMC: 11048163. DOI: 10.3390/bios14040200.

A lateral flow assay for miRNA-21 based on CRISPR/Cas13a and MnO nanosheets-mediated recognition and signal amplification.

Wang M, Cai S, Wu Y, Li Q, Wang X, Zhang Y Anal Bioanal Chem. 2024; 416(14):3401-3413.

PMID: 38630279 DOI: 10.1007/s00216-024-05290-0.

Development of nucleic acid lateral flow immunoassay for molecular detection of Entamoeba moshkovskii and Entamoeba dispar in stool samples.

Hutagalung S, Rattaprasert P, Promptmas C, Moonsom S, Yongkiettrakul S, Thima K Sci Rep. 2024; 14(1):6635.

PMID: 38503871 PMC: 10951296. DOI: 10.1038/s41598-024-57332-3.

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