Use of Synthetic Antigens to Determine the Epitope Specificities of Monoclonal Antibodies Against the 3-deoxy-D-manno-octulosonate Region of Bacterial Lipopolysaccharide

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1987 Feb 1

PMID 2433221

Citations 13

Authors

L Brade

P Kosma

B J Appelmelk

H Paulsen

H Brade

Affiliations

Soon will be listed here.

Abstract

Mouse monoclonal antibodies were raised against heat-killed bacteria of the Re mutant R595 of Salmonella minnesota and characterized by the passive hemolysis and passive hemolysis inhibition tests and by double immunodiffusion experiments using lipopolysaccharide (LPS) from different rough mutants of S. minnesota and synthetic antigens. The latter were copolymerization products of acrylamide with the alpha- and beta-allylglycosides of 3-deoxy-D-manno-octulosonic acid (KDO) and the alpha-2,4-linked KDO disaccharide [poly-alpha-KDO, poly-beta-KDO, and poly-(alpha-KDO)2, respectively], and sodium (3-deoxy-D-manno-octulopyranosyl)onate-(2----6)-(2-deoxy-2-[ (R)-3- hydroxytetradecanoylamino]- beta-D-glucopyranosyl)-(1----6)-(2-deoxy-2-[(R)-3-hydroxytetradecanoy lam ino]-D-glucose) [alpha-KDO-(GlcNhm)2], representing a part structure of Re LPS. One antibody (clone 20, immunoglobulin M) was found to recognize a terminal alpha-linked KDO residue, since it reacted in the passive hemolysis assay with alpha-KDO-(GlcNhm)2 and all LPS tested, it was inhibited by all synthetic antigens containing alpha-linked KDO residues, and it gave a reaction of identity with poly-alpha-KDO and poly-(alpha-KDO)2 in double immunodiffusion experiments. A second antibody (clone 25, immunoglobulin G3) was identified as specific for an alpha-2,4-linked KDO disaccharide, since it reacted in immunodiffusion exclusively with synthetic poly-(alpha-KDO)2 and not with the monosaccharide derivatives in either anomeric configuration, and it was inhibited only with poly-(alpha-KDO)2 and with LPS from S. minnesota R595 (Re) and R345 (Rb2). The reaction of this antibody with R345 LPS is attributed to the quantitative substitution with KDO disaccharide present as a side chain, which is not present in stoichiometric amounts in the other LPS.

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References

LUDERITZ O, STAUB A, Westphal O . Immunochemistry of O and R antigens of Salmonella and related Enterobacteriaceae. Bacteriol Rev. 1966; 30(1):192-255. PMC: 378223. DOI: 10.1128/br.30.1.192-255.1966. View

Elbein A, Heath E . THE BIOSYNTHESIS OF CELL WALL LIPOPOLYSACCHARIDE IN ESCHERICHIA COLI. I. THE BIOCHEMICAL PROPERTIES OF A URIDINE DIPHOSPHATE GALACTOSE 4-EPIMERASELESS MUTANT. J Biol Chem. 1965; 240:1919-25. View

Galanos C, LUDERITZ O, Westphal O . A new method for the extraction of R lipopolysaccharides. Eur J Biochem. 1969; 9(2):245-9. DOI: 10.1111/j.1432-1033.1969.tb00601.x. View

Galanos C, LUDERITZ O . Electrodialysis of lipopolysaccharides and their conversion to uniform salt forms. Eur J Biochem. 1975; 54(2):603-10. DOI: 10.1111/j.1432-1033.1975.tb04172.x. View

Ng A, Chen C, Chang C, Nowotny A . Relationship of structure to function in bacterial endotoxins: serologically cross-reactive components and their effect on protection of mice against some gram-negative infections. J Gen Microbiol. 1976; 94(1):107-16. DOI: 10.1099/00221287-94-1-107. View

Bruins S, Stumacher R, Johns M, McCabe W . Immunization with R mutants of Salmonella minnesota. II. Comparison of the protective effect of immunization with lipid A and the Re mutant. Infect Immun. 1977; 17(1):16-20. PMC: 421075. DOI: 10.1128/iai.17.1.16-20.1977. View

Johns M, Bruins S, McCabe W . Immunization with R mutants of Salmonella minnesota. II. Serological response to lipid A and the lipopolysaccharide of Re mutants. Infect Immun. 1977; 17(1):9-15. PMC: 421074. DOI: 10.1128/iai.17.1.9-15.1977. View

BRAUDE A, Ziegler E, Douglas H, McCutchan J . Antibody to cell wall glycolipid of Gram-negative bacteria: induction of immunity to bacteremia and endotoxemia. J Infect Dis. 1977; 136 Suppl:S167-73. DOI: 10.1093/infdis/136.supplement.s167. View

GREISMAN S, DuBuy J, Woodward C . Experimental gram-negative bacterial sepsis: reevaluation of the ability of rough mutant antisera to protect mice. Proc Soc Exp Biol Med. 1978; 158(3):482-90. DOI: 10.3181/00379727-158-40231. View

10.

Ziegler E, McCutchan J, Fierer J, Glauser M, Sadoff J, Douglas H . Treatment of gram-negative bacteremia and shock with human antiserum to a mutant Escherichia coli. N Engl J Med. 1982; 307(20):1225-30. DOI: 10.1056/NEJM198211113072001. View

11.

Brade H, Galanos C, LUDERITZ O . Differential determination of the 3-Deoxy-D-mannooctulosonic acid residues in lipopolysaccharides of Salmonella minnesota rough mutants. Eur J Biochem. 1983; 131(1):195-200. DOI: 10.1111/j.1432-1033.1983.tb07249.x. View

12.

Brade H, Galanos C, LUDERITZ O . Isolation of a 3-deoxy-D-mannooctulosonic acid disaccharide from Salmonella minnesota rough-form lipopolysaccharides. Eur J Biochem. 1983; 131(1):201-3. DOI: 10.1111/j.1432-1033.1983.tb07250.x. View

13.

Kirkland T, Ziegler E . An immunoprotective monoclonal antibody to lipopolysaccharide. J Immunol. 1984; 132(5):2590-2. View

14.

Chernyak AYa , Levinsky A, DMITRIEV B, Kochetkov N . A new type of carbohydrate-containing synthetic antigen: synthesis of carbohydrate-containing polyacrylamide copolymers having the specificity of O:3 and O:4 factors of Salmonella. Carbohydr Res. 1984; 128(2):269-82. DOI: 10.1016/0008-6215(84)85334-3. View

15.

Mutharia L, Crockford G, Bogard Jr W, Hancock R . Monoclonal antibodies specific for Escherichia coli J5 lipopolysaccharide: cross-reaction with other gram-negative bacterial species. Infect Immun. 1984; 45(3):631-6. PMC: 263341. DOI: 10.1128/iai.45.3.631-636.1984. View

16.

LUDERITZ O, Tanamoto K, Galanos C, McKenzie G, Brade H, Zahringer U . Lipopolysaccharides: structural principles and biologic activities. Rev Infect Dis. 1984; 6(4):428-31. DOI: 10.1093/clinids/6.4.428. View

17.

Brade H, Rietschel E . Alpha-2----4-interlinked 3-deoxy-D-manno-octulosonic acid disaccharide. A common constituent of enterobacterial lipopolysaccharides. Eur J Biochem. 1984; 145(2):231-6. DOI: 10.1111/j.1432-1033.1984.tb08543.x. View

18.

Nelles M, Niswander C . Mouse monoclonal antibodies reactive with J5 lipopolysaccharide exhibit extensive serological cross-reactivity with a variety of gram-negative bacteria. Infect Immun. 1984; 46(3):677-81. PMC: 261596. DOI: 10.1128/iai.46.3.677-681.1984. View

19.

Galanos C, LUDERITZ O, Rietschel E, Westphal O, Brade H, Brade L . Synthetic and natural Escherichia coli free lipid A express identical endotoxic activities. Eur J Biochem. 1985; 148(1):1-5. DOI: 10.1111/j.1432-1033.1985.tb08798.x. View

20.

Teng N, Kaplan H, Hebert J, Moore C, Douglas H, Wunderlich A . Protection against gram-negative bacteremia and endotoxemia with human monoclonal IgM antibodies. Proc Natl Acad Sci U S A. 1985; 82(6):1790-4. PMC: 397358. DOI: 10.1073/pnas.82.6.1790. View