Natural Killer Cell-mediated Host Defense Against Uropathogenic E. Coli is Counteracted by Bacterial HemolysinA-dependent Killing of NK Cells

Overview

Journal Cell Host Microbe

Publisher Cell Press

Specialties Infectious Diseases
Microbiology

Date 2013 Dec 17

PMID 24331464

Citations 38

Authors

Chamutal Gur

Shunit Coppenhagen-Glazer

Shilo Rosenberg

Rachel Yamin

Jonatan Enk

Ariella Glasner

Yotam Bar-On

Omer Fleissig

Ronit Naor

Jawad Abed

Dror Mevorach

Zvi Granot

Gilad Bachrach

Ofer Mandelboim

Affiliations

Soon will be listed here.

Abstract

Uropathogenic Escherichia coli (UPEC) are a common cause of urinary tract infections (UTIs) in humans. While the importance of natural killer (NK) cells in innate immune protection against tumors and viral infections is well documented, their role in defense against bacterial infections is still emerging, and their involvement in UPEC-mediated UTI is practically unknown. Using a systematic mutagenesis approach, we found that UPEC adheres to NK cells primarily via its type I fimbriae and employs its hemolysinA toxin to kill NK cells. In the absence of hemolysinA, NK cells directly respond to the bacteria and secrete the cytokine TNF-α, which results in decreased bacterial numbers in vitro and reduction of bacterial burden in the infected bladders. Thus, NK cells control UPEC via TNF-α production, which UPEC counteracts by hemolysinA-mediated killing of NK cells, representing a previously unrecognized host defense and microbial counterattack mechanism in the context of UTI.

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