Block of Calcium Channels by Enkephalin and Somatostatin in Neuroblastoma-glioma Hybrid NG108-15 Cells

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1986 Dec 1

PMID 2432604

Citations 65

Authors

A Tsunoo

M Yoshii

T Narahashi

Affiliations

Soon will be listed here.

Abstract

Leucine-enkephalin, methionine-enkephalin, and morphine caused a reversible block of Ca2+ channel currents in neuroblastoma-glioma hybrid cells (NG108-15). The long-lasting (type 2) component of the Ca2+ channel current was blocked by leucine-enkephalin, while the transient (type 1) component was not affected. The enkephalin-induced blocking action was antagonized by naloxone and appears to be mediated by delta-opiate receptors. Two different aspects of the blocking effect were detected, a resting block and a recovery from block during prolonged depolarizing pulses. Recovery from block was more complete, and its time course was more rapid, with depolarization to more positive potentials. The dose dependence of the type 2 channel block at rest indicated a one-to-one binding stoichiometry, with an apparent dissociation constant of 8.8 nM. Somatostatin exerted a similar selective blocking action on the type 2 Ca2+ channel. The time- and voltage-dependent block of type 2 Ca2+ channels may provide a mechanism underlying the enkephalinergic presynaptic inhibition of transmitter release and the somatostatin block of pituitary growth hormone release.

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References

Tsunoo A, Narahashi T . Cyclic AMP-mediated potentiation of muscarinic hyperpolarization in neuroblastoma cells. Brain Res. 1984; 294(1):123-6. DOI: 10.1016/0006-8993(84)91315-5. View

Tsien R . Calcium channels in excitable cell membranes. Annu Rev Physiol. 1983; 45:341-58. DOI: 10.1146/annurev.ph.45.030183.002013. View

Carbone E, Lux H . A low voltage-activated, fully inactivating Ca channel in vertebrate sensory neurones. Nature. 1984; 310(5977):501-2. DOI: 10.1038/310501a0. View

Carbone E, Lux H . A low voltage-activated calcium conductance in embryonic chick sensory neurons. Biophys J. 1984; 46(3):413-8. PMC: 1434947. DOI: 10.1016/S0006-3495(84)84037-0. View

Quandt F, Narahashi T . Isolation and kinetic analysis of inward currents in neuroblastoma cells. Neuroscience. 1984; 13(1):249-62. DOI: 10.1016/0306-4522(84)90275-6. View

Hamel E, Beaudet A . Electron microscopic autoradiographic localization of opioid receptors in rat neostriatum. Nature. 1984; 312(5990):155-7. DOI: 10.1038/312155a0. View

ARMSTRONG C, Matteson D . Two distinct populations of calcium channels in a clonal line of pituitary cells. Science. 1985; 227(4682):65-7. DOI: 10.1126/science.2578071. View

Werz M, Macdonald R . Dynorphin and neoendorphin peptides decrease dorsal root ganglion neuron calcium-dependent action potential duration. J Pharmacol Exp Ther. 1985; 234(1):49-56. View

Nowycky M, Fox A, Tsien R . Three types of neuronal calcium channel with different calcium agonist sensitivity. Nature. 1985; 316(6027):440-3. DOI: 10.1038/316440a0. View

10.

Nilius B, Hess P, Lansman J, Tsien R . A novel type of cardiac calcium channel in ventricular cells. Nature. 1985; 316(6027):443-6. DOI: 10.1038/316443a0. View

11.

Bean B . Two kinds of calcium channels in canine atrial cells. Differences in kinetics, selectivity, and pharmacology. J Gen Physiol. 1985; 86(1):1-30. PMC: 2228774. DOI: 10.1085/jgp.86.1.1. View

12.

Yoshimura M, North R . Substantia gelatinosa neurones hyperpolarized in vitro by enkephalin. Nature. 1983; 305(5934):529-30. DOI: 10.1038/305529a0. View

13.

BRAZEAU P, Vale W, Burgus R, Ling N, Butcher M, Rivier J . Hypothalamic polypeptide that inhibits the secretion of immunoreactive pituitary growth hormone. Science. 1973; 179(4068):77-9. DOI: 10.1126/science.179.4068.77. View

14.

Klee W, Nirenberg M . A neuroblastoma times glioma hybrid cell line with morphine receptors. Proc Natl Acad Sci U S A. 1974; 71(9):3474-7. PMC: 433796. DOI: 10.1073/pnas.71.9.3474. View

15.

Sharma S, Nirenberg M, Klee W . Morphine receptors as regulators of adenylate cyclase activity. Proc Natl Acad Sci U S A. 1975; 72(2):590-4. PMC: 432359. DOI: 10.1073/pnas.72.2.590. View

16.

Hughes J, Smith T, KOSTERLITZ H, Fothergill L, Morgan B, Morris H . Identification of two related pentapeptides from the brain with potent opiate agonist activity. Nature. 1975; 258(5536):577-80. DOI: 10.1038/258577a0. View

17.

Taube H, Borowski E, Endo T, Starke K . Enkephalin: a potential modulator of noradrenaline release in rat brain. Eur J Pharmacol. 1976; 38(2):377-80. DOI: 10.1016/0014-2999(76)90343-5. View

18.

Jessell T, Iversen L . Opiate analgesics inhibit substance P release from rat trigeminal nucleus. Nature. 1977; 268(5620):549-51. DOI: 10.1038/268549a0. View

19.

Traber J, Glaser T, Brandt M, Klebensberger W, Hamprecht B . Different receptors for somatostatin and opioids in neuroblastoma X glioma hybrid cells. FEBS Lett. 1977; 81(2):351-4. DOI: 10.1016/0014-5793(77)80552-8. View

20.

Macdonald R, Nelson P . Specific-opiate-induced depression of transmitter release from dorsal root ganglion cells in culture. Science. 1978; 199(4336):1449-51. DOI: 10.1126/science.204015. View