Correlation Between Myofibrillar ATPase Activity and Myosin Heavy Chain Composition in Rabbit Muscle Fibers

Overview

Journal Histochemistry

Specialty Biochemistry

Date 1986 Jan 1

PMID 2432036

Citations 65

Authors

R S Staron

D Pette

Affiliations

Soon will be listed here.

Abstract

Combined histochemical and biochemical analyses were performed on single fibers of rabbit soleus muscle. Histochemically, four fiber types (I, IC, IIC, IIA) were defined. Of these, types I and IIA were separate, histochemically homogeneous groups. A heterogeneous C fiber population exhibited a continuum of staining intensities between types I and IIA. Microelectrophoretic analyses of specific, histochemically defined fibers revealed that type I fibers contained exclusively HCI, whereas type IIA fibers contained only HCIIa. The C fibers were characterized by the coexistence of both heavy chains in varying ratios, type IC with a predominance of HCI and type IIC with a predominance of HCIIa. A direct correlation existed between the myosin heavy chain composition and the histochemical mATPase staining and was especially evident in the C fiber population with its variable HCI/HCIIa ratio. This correlation did not apply to the myosin light chain complement.

Citing Articles

Key concepts in muscle regeneration: muscle "cellular ecology" integrates a gestalt of cellular cross-talk, motility, and activity to remodel structure and restore function.

Anderson J Eur J Appl Physiol. 2021; 122(2):273-300.

PMID: 34928395 PMC: 8685813. DOI: 10.1007/s00421-021-04865-4.

Consequences of Maternal Essential Fatty Acid and Conjugated Linoleic Acid Supplementation on the Development of Calf Muscle and Adipose Tissue.

Dahl N, Albrecht E, Dannenberger D, Uken K, Hammon H, Maak S Animals (Basel). 2020; 10(9).

PMID: 32911793 PMC: 7552164. DOI: 10.3390/ani10091598.

Fiber-type distribution in insect leg muscles parallels similarities and differences in the functional role of insect walking legs.

Godlewska-Hammel E, Buschges A, Gruhn M J Comp Physiol A Neuroethol Sens Neural Behav Physiol. 2017; 203(10):773-790.

PMID: 28597315 DOI: 10.1007/s00359-017-1190-8.

A One-Step Immunostaining Method to Visualize Rodent Muscle Fiber Type within a Single Specimen.

Sawano S, Komiya Y, Ichitsubo R, Ohkawa Y, Nakamura M, Tatsumi R PLoS One. 2016; 11(11):e0166080.

PMID: 27814384 PMC: 5096669. DOI: 10.1371/journal.pone.0166080.

Histochemical study of rabbit medial pterygoid muscle during postnatal development.

Saito K, Morita T, Takasu H, Kuroki K, Fujiwara T, Hiraba K Odontology. 2016; 105(2):141-149.

PMID: 27456682 DOI: 10.1007/s10266-016-0262-5.

References

Billeter R, Weber H, Lutz H, Howald H, Eppenberger H, Jenny E . Myosin types in human skeletal muscle fibers. Histochemistry. 1980; 65(3):249-59. DOI: 10.1007/BF00493174. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Teutsch H . A new sample isolation procedure for microchemical analysis of functional liver cell heterogeneity. J Histochem Cytochem. 1986; 34(2):263-7. DOI: 10.1177/34.2.3003183. View

Guth L, SAMAHA F . Qualitative differences between actomyosin ATPase of slow and fast mammalian muscle. Exp Neurol. 1969; 25(1):138-52. DOI: 10.1016/0014-4886(69)90077-6. View

Brooke M, Kaiser K . Three "myosin adenosine triphosphatase" systems: the nature of their pH lability and sulfhydryl dependence. J Histochem Cytochem. 1970; 18(9):670-2. DOI: 10.1177/18.9.670. View

Carraro U, Catani C . A sensitive SDS-PAGE method separating myosin heavy chain isoforms of rat skeletal muscles reveals the heterogeneous nature of the embryonic myosin. Biochem Biophys Res Commun. 1983; 116(3):793-802. DOI: 10.1016/s0006-291x(83)80212-5. View

Mabuchi K, PINTER K, Mabuchi Y, SRETER F, Gergely J . Characterization of rabbit masseter muscle fibers. Muscle Nerve. 1984; 7(6):431-8. DOI: 10.1002/mus.880070603. View

Rushbrook J, Stracher A . Comparison of adult, embryonic, and dystrophic myosin heavy chains from chicken muscle by sodium dodecyl sulfate/polyacrylamide gel electrophoresis and peptide mapping. Proc Natl Acad Sci U S A. 1979; 76(9):4331-4. PMC: 411568. DOI: 10.1073/pnas.76.9.4331. View

Staron R, Pette D . The multiplicity of combinations of myosin light chains and heavy chains in histochemically typed single fibres. Rabbit soleus muscle. Biochem J. 1987; 243(3):687-93. PMC: 1147913. DOI: 10.1042/bj2430687. View

10.

Arndt I, Pepe F . Antigenic specificity of red and white muscle myosin. J Histochem Cytochem. 1975; 23(3):159-68. DOI: 10.1177/23.3.47867. View

11.

Pierobon-Bormioli S, Sartore S, Libera L, Vitadello M, Schiaffino S . "Fast" isomyosins and fiber types in mammalian skeletal muscle. J Histochem Cytochem. 1981; 29(10):1179-88. DOI: 10.1177/29.10.7028858. View

12.

Staron R, Pette D . The multiplicity of combinations of myosin light chains and heavy chains in histochemically typed single fibres. Rabbit tibialis anterior muscle. Biochem J. 1987; 243(3):695-9. PMC: 1147914. DOI: 10.1042/bj2430695. View

13.

Salviati G, Betto R, Danieli Betto D . Polymorphism of myofibrillar proteins of rabbit skeletal-muscle fibres. An electrophoretic study of single fibres. Biochem J. 1982; 207(2):261-72. PMC: 1153856. DOI: 10.1042/bj2070261. View

14.

Oakley B, Kirsch D, Morris N . A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Anal Biochem. 1980; 105(2):361-3. DOI: 10.1016/0003-2697(80)90470-4. View

15.

Reiser P, Moss R, Giulian G, Greaser M . Shortening velocity and myosin heavy chains of developing rabbit muscle fibers. J Biol Chem. 1985; 260(27):14403-5. View

16.

GAUTHIER G, LOWEY S . Polymorphism of myosin among skeletal muscle fiber types. J Cell Biol. 1977; 74(3):760-79. PMC: 2110104. DOI: 10.1083/jcb.74.3.760. View

17.

Billeter R, Heizmann C, Howald H, Jenny E . Analysis of myosin light and heavy chain types in single human skeletal muscle fibers. Eur J Biochem. 1981; 116(2):389-95. DOI: 10.1111/j.1432-1033.1981.tb05347.x. View

18.

GAUTHIER G, LOWEY S . Distribution of myosin isoenzymes among skeletal muscle fiber types. J Cell Biol. 1979; 81(1):10-25. PMC: 2111513. DOI: 10.1083/jcb.81.1.10. View

19.

Salviati G, Betto R, Danieli Betto D, Zeviani M . Myofibrillar-protein isoforms and sarcoplasmic-reticulum Ca2+-transport activity of single human muscle fibres. Biochem J. 1984; 224(1):215-25. PMC: 1144416. DOI: 10.1042/bj2240215. View

20.

Meschede K, Lehr I . Histochemical and immunochemical studies on mammalian striated muscle fibres. Histochemie. 1973; 33(1):79-85. DOI: 10.1007/BF00304229. View