Exogenous Nucleotides Antagonize the Developmental Toxicity of Ethanol in Vitro

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2013 Dec 10

PMID 24319676

Authors

Jie Zhao

Jia-Xi Zhao

Ya-Jun Xu

Affiliations

Soon will be listed here.

Abstract

The objective of this study was to assess whether nucleotides supplementation in vitro could suppress ethanol-induced developmental toxicity in mouse. The models of whole embryo culture (WEC) and midbrain (MB) cell micromass culture were used in this study. In WEC system, exposure to 4.0 mg/mL ethanol for 48 h yielded various developmental malformations of the mice embryos. Nucleotides supplementation (0.16, 0.80, 4.00, 20.00, and 100.00 mg/L) improved the growth parameters to some extent, and the protective effects peaked at 4.00 mg/L. In MB cell micromass culture system, exposure to 4.0 mg/mL ethanol for 5 days resulted in suppression of proliferation and differentiation. Supplementation of nucleotides (0.16, 0.80, 4.00, 20.00, and 100.00 mg/L) showed some protective effects, which peaked at 4.00 mg/L, too. The present research indicated that nucleotides supplementation might be of some benefit in the prevention of ethanol-induced birth defects; however, appropriate dosage requires attention.

References

Welch-Carre E . The neurodevelopmental consequences of prenatal alcohol exposure. Adv Neonatal Care. 2005; 5(4):217-29. DOI: 10.1016/j.adnc.2005.04.007. View

Green J . Fetal Alcohol Spectrum Disorders: understanding the effects of prenatal alcohol exposure and supporting students. J Sch Health. 2007; 77(3):103-8. DOI: 10.1111/j.1746-1561.2007.00178.x. View

Niccols A . Fetal alcohol syndrome and the developing socio-emotional brain. Brain Cogn. 2007; 65(1):135-42. DOI: 10.1016/j.bandc.2007.02.009. View

Kaemingk K, Halverson P . Spatial memory following prenatal alcohol exposure: more than a material specific memory deficit. Child Neuropsychol. 2005; 6(2):115-28. DOI: 10.1076/chin.6.2.115.7058. View

Cosgrove M, Davies D, Jenkins H . Nucleotide supplementation and the growth of term small for gestational age infants. Arch Dis Child Fetal Neonatal Ed. 1996; 74(2):F122-5. PMC: 2528517. DOI: 10.1136/fn.74.2.f122. View

Van Maele-Fabry G, Delhaise F, Picard J . Morphogenesis and quantification of the development of post-implantation mouse embryos. Toxicol In Vitro. 2010; 4(2):149-56. DOI: 10.1016/0887-2333(90)90037-t. View

Xu Y, Li L, Zhang Z, Li Y . Effects of folinic acid and Vitamin B12 on ethanol-induced developmental toxicity in mouse. Toxicol Lett. 2006; 167(3):167-72. DOI: 10.1016/j.toxlet.2006.07.341. View

Caldwell K, Sheema S, Paz R, Samudio-Ruiz S, Laughlin M, Spence N . Fetal alcohol spectrum disorder-associated depression: evidence for reductions in the levels of brain-derived neurotrophic factor in a mouse model. Pharmacol Biochem Behav. 2008; 90(4):614-24. PMC: 2762406. DOI: 10.1016/j.pbb.2008.05.004. View

NEW D . Whole-embryo culture and the study of mammalian embryos during organogenesis. Biol Rev Camb Philos Soc. 1978; 53(1):81-122. DOI: 10.1111/j.1469-185x.1978.tb00993.x. View

10.

Kvigne V, Leonardson G, Neff-Smith M, Brock E, Borzelleca J, Welty T . Characteristics of children who have full or incomplete fetal alcohol syndrome. J Pediatr. 2004; 145(5):635-40. DOI: 10.1016/j.jpeds.2004.07.015. View

11.

Sato N, Murakami Y, Nakano T, Sugawara M, Kawakami H, Idota T . Effects of dietary nucleotides on lipid metabolism and learning ability of rats. Biosci Biotechnol Biochem. 1995; 59(7):1267-71. DOI: 10.1271/bbb.59.1267. View

12.

Prut L, Belzung C . The open field as a paradigm to measure the effects of drugs on anxiety-like behaviors: a review. Eur J Pharmacol. 2003; 463(1-3):3-33. DOI: 10.1016/s0014-2999(03)01272-x. View

13.

Xu Y, Tang Y, Li Y . Effect of folic acid on prenatal alcohol-induced modification of brain proteome in mice. Br J Nutr. 2007; 99(3):455-61. DOI: 10.1017/S0007114507812074. View

14.

Sharpe T, Alexander M, Hutcherson J, Floyd R, Brimacombe M, Levine R . Report from the CDC. Physician and allied health professionals' training and fetal alcohol syndrome. J Womens Health (Larchmt). 2004; 13(2):133-9. DOI: 10.1089/154099904322966100. View

15.

Wang J, Giacomini K . Molecular determinants of substrate selectivity in Na+-dependent nucleoside transporters. J Biol Chem. 1997; 272(46):28845-8. DOI: 10.1074/jbc.272.46.28845. View

16.

Yu V . Scientific rationale and benefits of nucleotide supplementation of infant formula. J Paediatr Child Health. 2002; 38(6):543-9. DOI: 10.1046/j.1440-1754.2002.00056.x. View

17.

Xu Y, Chen X, Li Y . Ercc6l, a gene of SNF2 family, may play a role in the teratogenic action of alcohol. Toxicol Lett. 2005; 157(3):233-9. DOI: 10.1016/j.toxlet.2005.02.013. View

18.

Xu Y, Xiao R, Li Y . Effect of ethanol on the development of visceral yolk sac. Hum Reprod. 2005; 20(9):2509-16. DOI: 10.1093/humrep/dei075. View

19.

Abate P, Pueta M, Spear N, Molina J . Fetal learning about ethanol and later ethanol responsiveness: evidence against "safe" amounts of prenatal exposure. Exp Biol Med (Maywood). 2008; 233(2):139-54. PMC: 2661129. DOI: 10.3181/0703-MR-69. View

20.

Tough S, Tofflemire K, Clarke M, Newburn-Cook C . Do women change their drinking behaviors while trying to conceive? An opportunity for preconception counseling. Clin Med Res. 2006; 4(2):97-105. PMC: 1483891. DOI: 10.3121/cmr.4.2.97. View