A Family of Wheat Embryo U2 SnRNAs

Overview

Journal Plant Mol Biol

Date 2013 Dec 7

PMID 24310755

Citations 1

Authors

J M Skuzeski

J J Jendrisak

Affiliations

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Abstract

Evidence is presented for the existence of small nuclear RNAs in a higher plant species. Based on subcellular fractionation experiments, wheat embryos contain at least four putative snRNAs, one of which co-migrates on SDS-polyacrylamide gels with a relatively abundant cytoplasmic RNA, W1. We purified W1 from ribosome-free high speed supernatant fractions for characterization studies. Electrophoresis under partially denaturing conditions resolves this RNA into several components which bear m3 (2, 2, 7) G-5' caps and strongly resemble vertebrate U2 snRNA on the basis of modified nucleotide content. Preliminary sequence analyses indicate that wheat embryos contain at least three U2-like RNAs which possess slightly different sequences near their 3' ends.

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PMID: 8358031 DOI: 10.1007/BF00027367.

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