Expression of Shoot-inducing Ti TL-DNA in Differentiated Tissues of Potato (Solanum Tuberosum Cv Maris Bard)

Overview

Journal Plant Mol Biol

Date 2013 Dec 6

PMID 24306762

Citations 12

Authors

M M Burrell

D Twell

A Karp

G Ooms

Affiliations

Soon will be listed here.

Abstract

In potato line Mb1501B one or possibly two normal size Ti TL-DNA copies per tetraploid genome were detected by Southern blot analysis, but no TR-DNA. The TL-DNA probably contained the entire transposon Tn1831 inserted at the T-DNA auxin gene for transcript 2. Northern blot analyses of the steady-state RNA in different Mb1501B tissues isolated from (i) shoots cultured in vitro (ii) grafted plants and (iii) tubers, showed that that TL-DNA transcripts 3, 4, 6a and 7 were expressed most abundantly in the cultured shoots. They formed approximately 0.0023 to 0.0007% of the total poly(A) RNA. Transcripts 1, 5 and 6b were not detected in any of the tissues analysed. This indicated even lower levels of expression (below approximately 0.0001% of the total poly(A) RNA or, making certain assumptions, an abundance of less than one T-DNA derived RNA molecule per cell). As expected, transcript 2 was not detected in any of the Mb1501B tissues. The abundance of the transcripts was reduced in grafted plants and tubers compared with cultured shoots with the greatest decrease (5×) for transcripts 4, 6a and 7. Transcript 4, the one most responsible for the changed growth and development of Mb 1501 B, formed approximately 0.0003% of the poly(A) RNA from both grafted plants and tubers.

Citing Articles

Developmental regulation of RI TL-DNA gene expression in roots, shoots and tubers of transformed potato (Solanum tuberosum cv. Desiree).

Ooms G, Twell D, Bossen M, Hoge J, Burrell M Plant Mol Biol. 2013; 6(5):321-30.

PMID: 24307382 DOI: 10.1007/BF00034939.

Changes in translatable poly(A) RNA from differentiated potato tissues transformed with shoot-inducing Ti TL-DNA of Agrobacterium tumefaciens.

Burrell M, Temple S, Ooms G Plant Mol Biol. 2013; 6(4):213-20.

PMID: 24307320 DOI: 10.1007/BF00015227.

T-DNA genes to study plant development: precocious tuberisation and enhanced cytokinins in A. tumefaciens transformed potato.

Ooms G, Lenton J Plant Mol Biol. 2013; 5(4):205-12.

PMID: 24306761 DOI: 10.1007/BF00020638.

Clones from a shooty tobacco crown gall tumor I: deletions, rearrangements and amplifications resulting in irregular T-DNA structures and organizations.

Peerbolte R, Leenhouts K, Hooykaas-van Slogteren G, Hoge J, Wullems G, Schilperoort R Plant Mol Biol. 2013; 7(4):265-84.

PMID: 24302369 DOI: 10.1007/BF00752900.

The chloroplast-located glutamine synthetase of Phaseolus vulgaris L.: nucleotide sequence, expression in different organs and uptake into isolated chloroplasts.

Lightfoot D, Green N, Cullimore J Plant Mol Biol. 2013; 11(2):191-202.

PMID: 24272261 DOI: 10.1007/BF00015671.

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