Nucleotide Sequence of the Cytochrome Oxidase Subunit I Gene from Soybean Mitochondria

Overview

Journal Plant Mol Biol

Date 2013 Dec 5

PMID 24302407

Citations 8

Authors

E A Grabau

Affiliations

Soon will be listed here.

Abstract

The cytochrome oxidase subunit I gene was isolated from a soybean mitochondrial library and subcloned into M13 for DNA sequencing. The sequences of the gene and flanking regions are presented and compared to the corresponding gene from maize. There is approximately 94% sequence homology between the soybean (dicot) and maize (monocot) coding sequences at the nucleotide level. The soybean sequence exists as a single copy in the mitochondrial genome and contains an open reading frame that could encode a polypeptide of 527 amino acids. There is very little sequence homology between the soybean and maize sequences upstream from the coding regions and none is detected downstream. Even the 3' ends of the COI coding regions differ considerably between soybean and maize. There are many amino acid differences at the carboxy terminus and the predicted polypeptide contains one less amino acid than the maize sequence. Northern analysis of the soybean mitochondrial RNA suggests that this region is actively transcribed and yields two major transcripts.

Citing Articles

Creation of an initiation codon by RNA editing in the coxI transcript from tomato mitochondria.

Kadowaki K, Ozawa K, Kazama S, Kubo N, Akihama T Curr Genet. 1995; 28(5):415-22.

PMID: 8575013 DOI: 10.1007/BF00310809.

Molecular phylogeny of Allomyces macrogynus: congruency between nuclear ribosomal RNA- and mitochondrial protein-based trees.

Paquin B, Forget L, Roewer I, Lang B J Mol Evol. 1995; 41(5):657-65.

PMID: 7490780 DOI: 10.1007/BF00175824.

The ATPase subunit 6 gene of tobacco mitochondria contains an unusual sequence.

Bland M, Levings 3rd C, Matzinger D Curr Genet. 1987; 12(7):475-81.

PMID: 2896073 DOI: 10.1007/BF00419555.

Chimeric organization of two genes for the soybean mitochondrial ATPase subunit 6.

Grabau E, Havlik M, Gesteland R Curr Genet. 1988; 13(1):83-9.

PMID: 2834107 DOI: 10.1007/BF00365761.

Isolation and nucleotide sequence of the pea cytochrome oxidase subunit I gene.

Kemmerer E, Kao T, Deng G, Wu R Plant Mol Biol. 1989; 13(1):121-4.

PMID: 2562779 DOI: 10.1007/BF00027340.

References

Grabau E . Nucleotide sequence of the soybean mitochondrial 18S rRNA gene: evidence for a slow rate of divergence in the plant mitochondrial genome. Plant Mol Biol. 2013; 5(2):119-24. DOI: 10.1007/BF00020094. View

Spencer D, Schnare M, Gray M . Pronounced structural similarities between the small subunit ribosomal RNA genes of wheat mitochondria and Escherichia coli. Proc Natl Acad Sci U S A. 1984; 81(2):493-7. PMC: 344704. DOI: 10.1073/pnas.81.2.493. View

Morgens P, Grabau E, GESTELAND R . A novel soybean mitochondrial transcript resulting from a DNA rearrangement involving the 5S rRNA gene. Nucleic Acids Res. 1984; 12(14):5665-84. PMC: 320022. DOI: 10.1093/nar/12.14.5665. View

KYTE J, Doolittle R . A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982; 157(1):105-32. DOI: 10.1016/0022-2836(82)90515-0. View

Burger G, Scriven C, Machleidt W, Werner S . Subunit 1 of cytochrome oxidase from Neurospora crassa: nucleotide sequence of the coding gene and partial amino acid sequence of the protein. EMBO J. 1982; 1(11):1385-91. PMC: 553221. DOI: 10.1002/j.1460-2075.1982.tb01327.x. View

Dewey R, Schuster A, Levings C, Timothy D . Nucleotide sequence of F(0)-ATPase proteolipid (subunit 9) gene of maize mitochondria. Proc Natl Acad Sci U S A. 1985; 82(4):1015-9. PMC: 397184. DOI: 10.1073/pnas.82.4.1015. View

Bonen L, Boer P, Gray M . The wheat cytochrome oxidase subunit II gene has an intron insert and three radical amino acid changes relative to maize. EMBO J. 1984; 3(11):2531-6. PMC: 557724. DOI: 10.1002/j.1460-2075.1984.tb02168.x. View

Brown W, Prager E, Wang A, Wilson A . Mitochondrial DNA sequences of primates: tempo and mode of evolution. J Mol Evol. 1982; 18(4):225-39. DOI: 10.1007/BF01734101. View

Bonitz S, Coruzzi G, Thalenfeld B, Tzagoloff A, Macino G . Assembly of the mitochondrial membrane system. Structure and nucleotide sequence of the gene coding for subunit 1 of yeast cytochrme oxidase. J Biol Chem. 1980; 255(24):11927-41. View

10.

de Bruijn M . Drosophila melanogaster mitochondrial DNA, a novel organization and genetic code. Nature. 1983; 304(5923):234-41. DOI: 10.1038/304234a0. View

11.

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

12.

Boer P, McIntosh J, Gray M, Bonen L . The wheat mitochondrial gene for apocytochrome b: absence of a prokaryotic ribosome binding site. Nucleic Acids Res. 1985; 13(7):2281-92. PMC: 341155. DOI: 10.1093/nar/13.7.2281. View

13.

Hiesel R, Brennicke A . Cytochrome oxidase subunit II gene in mitochondria of Oenothera has no intron. EMBO J. 1983; 2(12):2173-8. PMC: 555430. DOI: 10.1002/j.1460-2075.1983.tb01719.x. View

14.

Anderson S, Bankier A, Barrell B, de Bruijn M, Coulson A, Drouin J . Sequence and organization of the human mitochondrial genome. Nature. 1981; 290(5806):457-65. DOI: 10.1038/290457a0. View

15.

Fox T, Leaver C . The Zea mays mitochondrial gene coding cytochrome oxidase subunit II has an intervening sequence and does not contain TGA codons. Cell. 1981; 26(3 Pt 1):315-23. DOI: 10.1016/0092-8674(81)90200-2. View

16.

Dewey R, Levings C, Timothy D . Nucleotide sequence of ATPase subunit 6 gene of maize mitochondria. Plant Physiol. 1985; 79(3):914-9. PMC: 1074995. DOI: 10.1104/pp.79.3.914. View

17.

Dawson A, Jones V, Leaver C . The apocytochrome b gene in maize mitochondria does not contain introns and is preceded by a potential ribosome binding site. EMBO J. 1984; 3(9):2107-13. PMC: 557650. DOI: 10.1002/j.1460-2075.1984.tb02098.x. View

18.

Kao T, Moon E, Wu R . Cytochrome oxidase subunit II gene of rice has an insertion sequence within the intron. Nucleic Acids Res. 1984; 12(19):7305-15. PMC: 320163. DOI: 10.1093/nar/12.19.7305. View

19.

Rigby P, Dieckmann M, Rhodes C, Berg P . Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977; 113(1):237-51. DOI: 10.1016/0022-2836(77)90052-3. View

20.

Isaac P, Jones V, Leaver C . The maize cytochrome c oxidase subunit I gene: sequence, expression and rearrangement in cytoplasmic male sterile plants. EMBO J. 1985; 4(7):1617-23. PMC: 554395. DOI: 10.1002/j.1460-2075.1985.tb03828.x. View