Hepatitis B Virus Large Surface Protein is Not Secreted but is Immunogenic when Selectively Expressed by Recombinant Vaccinia Virus

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1986 Nov 1

PMID 2430108

Citations 33

Authors

K C Cheng

G L Smith

B Moss

Affiliations

Soon will be listed here.

Abstract

The envelope region of the hepatitis B virus (HBV) genome contains an open reading frame that begins upstream of the major surface protein gene. The two minor proteins that are initiated within this pre-s segment are immunogenic and may be involved in virus attachment to hepatocytes. We have constructed a recombinant vaccinia virus that contains the predicted coding segment for the large surface protein (LS) under control of a vaccinia virus that contains the predicted coding segment for the large surface protein (LS) under control of a vaccinia virus promoter. Cells infected with the recombinant virus synthesized HBV polypeptides of 39 and 42 kilodaltons, corresponding to the unglycosylated and glycosylated forms of LS, respectively. The presence of pre-s epitopes in the 39- and 42-kilodalton polypeptides was demonstrated by binding of antibody prepared against a synthetic peptide. Synthesis of the 42-kilodalton species was specifically inhibited by tunicamycin, suggesting that it is N-glycosylated. Despite apparent glycosylation, LS was not secreted into the medium of infected cells. Nevertheless, rabbits vaccinated with the purified recombinant virus made antibodies that recognized s and pre-s epitopes. Antibody to the NH2 terminus of LS appeared before or simultaneously with antibody that bound to the major surface protein. The additional immunogenicity provided by expression of LS may be advantageous for the development of an HBV vaccine.

Citing Articles

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Posttranslational modifications and secretion efficiency of immunogenic hepatitis B virus L protein deletion variants.

Niedre-Otomere B, Bogdanova A, Bruvere R, Ose V, Gerlich W, Pumpens P Virol J. 2013; 10:63.

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Removing N-terminal sequences in pre-S1 domain enhanced antibody and B-cell responses by an HBV large surface antigen DNA vaccine.

Ge G, Wang S, Han Y, Zhang C, Lu S, Huang Z PLoS One. 2012; 7(7):e41573.

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Immunoadhesins containing pre-S domains of hepatitis B virus large envelope protein are secreted and inhibit virus infection.

Chai N, Gudima S, Chang J, Taylor J J Virol. 2007; 81(10):4912-8.

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Molecular chaperone GRP78/BiP interacts with the large surface protein of hepatitis B virus in vitro and in vivo.

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