Epithelial Bridges Maintain Tissue Integrity During Collective Cell Migration

Overview

Journal Nat Mater

Date 2013 Dec 3

PMID 24292420

Citations 83

Authors

Sri Ram Krishna Vedula

Hiroaki Hirata

Mui Hoon Nai

Agusti Brugues

Yusuke Toyama

Xavier Trepat

Chwee Teck Lim

Benoit Ladoux

Affiliations

Soon will be listed here.

Abstract

The ability of skin to act as a barrier is primarily determined by the efficiency of skin cells to maintain and restore its continuity and integrity. In fact, during wound healing keratinocytes migrate collectively to maintain their cohesion despite heterogeneities in the extracellular matrix. Here, we show that monolayers of human keratinocytes migrating along functionalized micropatterned surfaces comprising alternating strips of extracellular matrix (fibronectin) and non-adherent polymer form suspended multicellular bridges over the non-adherent areas. The bridges are held together by intercellular adhesion and are subjected to considerable tension, as indicated by the presence of prominent actin bundles. We also show that a model based on force propagation through an elastic material reproduces the main features of bridge maintenance and tension distribution. Our findings suggest that multicellular bridges maintain tissue integrity during wound healing when cell-substrate interactions are weak and may prove helpful in the design of artificial scaffolds for skin regeneration.

Citing Articles

Different Biomechanical Cell Behaviors in an Epithelium Drive Collective Epithelial Cell Extrusion.

Balasubramaniam L, Jain S, Dang T, Lagoutte E, Marc Mege R, Chavrier P Adv Sci (Weinh). 2024; 11(42):e2401573.

PMID: 39291385 PMC: 11558136. DOI: 10.1002/advs.202401573.

Patterning in stratified epithelia depends on cell-cell adhesion.

Mai Y, Kobayashi Y, Kitahata H, Seo T, Nohara T, Itamoto S Life Sci Alliance. 2024; 7(9).

PMID: 39025524 PMC: 11258421. DOI: 10.26508/lsa.202402893.

Pulmonary maternal immune activation does not cross the placenta but leads to fetal metabolic adaptation.

Schmidt Kjolner Hansen S, Krautz R, Rago D, Havelund J, Stigliani A, Faergeman N Nat Commun. 2024; 15(1):4711.

PMID: 38830841 PMC: 11148039. DOI: 10.1038/s41467-024-48492-x.

Force propagation between epithelial cells depends on active coupling and mechano-structural polarization.

Ruppel A, Worthmuller D, Misiak V, Kelkar M, Wang I, Moreau P Elife. 2023; 12.

PMID: 37548995 PMC: 10511242. DOI: 10.7554/eLife.83588.

Regeneration in calcareous sponge relies on 'purse-string' mechanism and the rearrangements of actin cytoskeleton.

Skorentseva K, Bolshakov F, Saidova A, Lavrov A Cell Tissue Res. 2023; 394(1):107-129.

PMID: 37466725 DOI: 10.1007/s00441-023-03810-5.

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