Enhancement of UVB Radiation-mediated Apoptosis by Knockdown of Cytosolic NADP+-dependent Isocitrate Dehydrogenase in HaCaT Cells

Overview

Journal BMB Rep

Specialties Biochemistry
Molecular Biology

Date 2013 Nov 30

PMID 24286310

Citations 5

Authors

Su Jeong Lee

Jeen-Woo Park

Affiliations

Soon will be listed here.

Abstract

Ultraviolet B (UVB) radiation induces the production of reactive oxygen species (ROS) that promote apoptotic cell death. We showed that cytosolic NADP+-dependent isocitrate dehydrogenase (IDPc) plays an essential role in the control of cellular redox balance and defense against oxidative damage, by supplying NADPH for antioxidant systems. In this study, we demonstrated that knockdown of IDPc expression by RNA interference enhances UVB-induced apoptosis of immortalized human HaCaT keratinocytes. This effect manifested as DNA fragmentation, changes in cellular redox status, mitochondrial dysfunction, and modulation of apoptotic marker expression. Based on our findings, we suggest that attenuation of IDPc expression may protect skin from UVB-mediated damage, by inducing the apoptosis of UV-damaged cells.

Citing Articles

Suppression of the inflammatory response by oxyresveratrol from the root bark of Roxb against ultraviolet B-induced keratinocytes mediated by regulating p38 MAPK and AP-1.

Malaniyom K, Ratanachamnong P, Namchaiw P, Namdaung U, Suksamrarn S, Jaisin Y Heliyon. 2024; 10(20):e38962.

PMID: 39469685 PMC: 11513453. DOI: 10.1016/j.heliyon.2024.e38962.

Heat-Killed Ameliorated UVB-Induced Oxidative Damage and Photoaging and Its Underlying Mechanisms.

Xu J, Zhang X, Song Y, Zheng B, Wen Z, Gong M Antioxidants (Basel). 2022; 11(10).

PMID: 36290598 PMC: 9598452. DOI: 10.3390/antiox11101875.

Isocitrate dehydrogenase 1-snail axis dysfunction significantly correlates with breast cancer prognosis and regulates cell invasion ability.

Liu W, Chan S, Chang H, Li G, Tu Y, Tseng H Breast Cancer Res. 2018; 20(1):25.

PMID: 29661250 PMC: 5902927. DOI: 10.1186/s13058-018-0953-7.

Baicalein Protects Human Skin Cells against Ultraviolet B-Induced Oxidative Stress.

Oh M, Piao M, Fernando P, Han X, Hewage S, Park J Biomol Ther (Seoul). 2016; 24(6):616-622.

PMID: 27257012 PMC: 5098541. DOI: 10.4062/biomolther.2016.022.

Saussurea tridactyla Sch. Bip.-derived polysaccharides and flavones reduce oxidative damage in ultraviolet B-irradiated HaCaT cells via a p38MAPK-independent mechanism.

Guo Y, Sun J, Ye J, Ma W, Yan H, Wang G Drug Des Devel Ther. 2016; 10:389-403.

PMID: 26855564 PMC: 4725635. DOI: 10.2147/DDDT.S96581.

References

Tamm I, Wang Y, Sausville E, Scudiero D, Vigna N, Oltersdorf T . IAP-family protein survivin inhibits caspase activity and apoptosis induced by Fas (CD95), Bax, caspases, and anticancer drugs. Cancer Res. 1998; 58(23):5315-20. View

Cao C, Lu S, Kivlin R, Wallin B, Card E, Bagdasarian A . AMP-activated protein kinase contributes to UV- and H2O2-induced apoptosis in human skin keratinocytes. J Biol Chem. 2008; 283(43):28897-908. PMC: 2570892. DOI: 10.1074/jbc.M804144200. View

An S, Kang J . Oxidative damage of DNA induced by the reaction of methylglyoxal with lysine in the presence of ferritin. BMB Rep. 2013; 46(4):225-9. PMC: 4133886. DOI: 10.5483/bmbrep.2013.46.4.225. View

Nohl H, Gille L, Staniek K . Intracellular generation of reactive oxygen species by mitochondria. Biochem Pharmacol. 2005; 69(5):719-23. DOI: 10.1016/j.bcp.2004.12.002. View

Slee E, Harte M, Kluck R, Wolf B, Casiano C, Newmeyer D . Ordering the cytochrome c-initiated caspase cascade: hierarchical activation of caspases-2, -3, -6, -7, -8, and -10 in a caspase-9-dependent manner. J Cell Biol. 1999; 144(2):281-92. PMC: 2132895. DOI: 10.1083/jcb.144.2.281. View

Sies H . Glutathione and its role in cellular functions. Free Radic Biol Med. 1999; 27(9-10):916-21. DOI: 10.1016/s0891-5849(99)00177-x. View

Mates J . Effects of antioxidant enzymes in the molecular control of reactive oxygen species toxicology. Toxicology. 2000; 153(1-3):83-104. DOI: 10.1016/s0300-483x(00)00306-1. View

Reagan-Shaw S, Breur J, Ahmad N . Enhancement of UVB radiation-mediated apoptosis by sanguinarine in HaCaT human immortalized keratinocytes. Mol Cancer Ther. 2006; 5(2):418-29. DOI: 10.1158/1535-7163.MCT-05-0250. View

Salvesen G, Dixit V . Caspases: intracellular signaling by proteolysis. Cell. 1997; 91(4):443-6. DOI: 10.1016/s0092-8674(00)80430-4. View

10.

Scharffetter-Kochanek K, Wlaschek M, Brenneisen P, Schauen M, Blaudschun R, Wenk J . UV-induced reactive oxygen species in photocarcinogenesis and photoaging. Biol Chem. 1998; 378(11):1247-57. View

11.

Nakamura H . Thioredoxin and its related molecules: update 2005. Antioxid Redox Signal. 2005; 7(5-6):823-8. DOI: 10.1089/ars.2005.7.823. View

12.

Lemasters J, Nieminen A, Qian T, Trost L, Elmore S, Nishimura Y . The mitochondrial permeability transition in cell death: a common mechanism in necrosis, apoptosis and autophagy. Biochim Biophys Acta. 1998; 1366(1-2):177-96. DOI: 10.1016/s0005-2728(98)00112-1. View

13.

Kirsch M, De Groot H . NAD(P)H, a directly operating antioxidant?. FASEB J. 2001; 15(9):1569-74. DOI: 10.1096/fj.00-0823hyp. View

14.

Williams M, Van Remmen H, Conrad C, Huang T, Epstein C, Richardson A . Increased oxidative damage is correlated to altered mitochondrial function in heterozygous manganese superoxide dismutase knockout mice. J Biol Chem. 1998; 273(43):28510-5. DOI: 10.1074/jbc.273.43.28510. View

15.

Kulms D, Zeise E, Poppelmann B, Schwarz T . DNA damage, death receptor activation and reactive oxygen species contribute to ultraviolet radiation-induced apoptosis in an essential and independent way. Oncogene. 2002; 21(38):5844-51. DOI: 10.1038/sj.onc.1205743. View

16.

Rodriguez C, Mayo J, Sainz R, Antolin I, Herrera F, Martin V . Regulation of antioxidant enzymes: a significant role for melatonin. J Pineal Res. 2003; 36(1):1-9. DOI: 10.1046/j.1600-079x.2003.00092.x. View

17.

Reers M, Smiley S, Chen A, Lin M, Chen L . Mitochondrial membrane potential monitored by JC-1 dye. Methods Enzymol. 1995; 260:406-17. DOI: 10.1016/0076-6879(95)60154-6. View

18.

Rezvani H, Mazurier F, Cario-Andre M, Pain C, Ged C, Taieb A . Protective effects of catalase overexpression on UVB-induced apoptosis in normal human keratinocytes. J Biol Chem. 2006; 281(26):17999-8007. DOI: 10.1074/jbc.M600536200. View

19.

Lee S, Koh H, Park D, Song B, Huh T, Park J . Cytosolic NADP(+)-dependent isocitrate dehydrogenase status modulates oxidative damage to cells. Free Radic Biol Med. 2002; 32(11):1185-96. DOI: 10.1016/s0891-5849(02)00815-8. View

20.

Tak J, Park J . The use of ebselen for radioprotection in cultured cells and mice. Free Radic Biol Med. 2009; 46(8):1177-85. DOI: 10.1016/j.freeradbiomed.2009.01.023. View