Transformation of Cotton (Gossypium Hirsutum L.) by Agrobacterium Tumefaciens and Regeneration of Transgenic Plants

Overview

Journal Plant Mol Biol

Date 2013 Nov 27

PMID 24277496

Citations 31

Authors

E Firoozabady

D L Deboer

D J Merlo

E L Halk

L N Amerson

K E Rashka

E E Murray

Affiliations

Soon will be listed here.

Abstract

Cotton (Gossypium hirsutum L.) cotyledon tissues have been efficiently transformed and plants have been regenerated. Cotyledon pieces from 12-day-old aseptically germinated seedlings were inoculated with Agrobacterium tumefaciens strains containing avirulent Ti (tumor-inducing) plasmids with a chimeric gene encoding kanamycin resistance. After three days cocultivation, the cotyledon pieces were placed on a callus initiation medium containing kanamycin for selection. High frequencies of transformed kanamycin-resistant calli were produced, more than 80% of which were induced to form somatic embryos. Somatic embryos were germinated, and plants were regenerated and transferred to soil. Transformation was confirmed by opine production, kanamycin resistance, immunoassay, and DNA blot hybridization. This process for producing transgenic cotton plants facilitates transfer of genes of economic importance to cotton.

Citing Articles

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