Transformation of Cotton (Gossypium Hirsutum L.) Via Particle Bombardment

Overview

Journal Plant Cell Rep

Publisher Springer

Date 2013 Nov 16

PMID 24232677

Citations 39

Authors

J J Finer

M D McMullen

Affiliations

Soon will be listed here.

Abstract

Embryogenic suspension cultures of cotton (Gossypium hirsutum L.) were subjected to particle bombardment, where high density particles carrying plasmid DNA were accelerated towards the embryogenic plant cells. The plasmid DNA coating the particles encoded hygromycin resistance. One to two weeks following bombardment, embryogenic cotton cells were placed in proliferation medium containing 100 μg/ml hygromycin. Clumps of tissue which grew in the presence of hygromycin were subcultured at low density into fresh hygromycin-containing proliferation medium. Following sequential transfer of embryogenic tissue to development and then germination media, plants were recovered from transgenic embryogenic tissue. Southern hybridization confirmed the presence of the hygromycin resistance gene in embryogenic suspension culture tissue and regenerated plants.

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