Proofreading of Noncognate Acyl Adenylates by an Acyl-coenzyme a Ligase

Overview

Journal Chem Biol

Publisher Elsevier

Specialties Biochemistry
Biology
Chemistry

Date 2013 Nov 26

PMID 24269150

Citations 11

Authors

Miglena Manandhar

John E Cronan

Affiliations

Soon will be listed here.

Abstract

Aminoacyl-tRNA synthetases remove (proofread) incorrect substrates and thereby prevent errors in protein synthesis. We report enzyme-catalyzed pretransfer editing by pimeloyl-coenzyme A (CoA) ligase (BioW), a biotin synthetic enzyme that converts pimelate, a seven-carbon dicarboxylic acid, to its CoA ester. The noncognate BioW substrate glutaric acid results in hydrolysis of ATP to AMP with formation of only trace amounts of glutaryl-CoA, thereby mimicking pretransfer editing of incorrect aminoacyl-adenylates by aminoacyl-tRNA synthetases.

Citing Articles

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Xu Y, Yang J, Li W, Song S, Shi Y, Wu L PLoS Pathog. 2022; 18(7):e1010615.

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BioWF: A Naturally-Fused, Di-Domain Biocatalyst from Biotin Biosynthesis Displays an Unexpectedly Broad Substrate Scope.

Richardson S, Harrison P, Herrera M, Wang M, Verez R, Ortiz G Chembiochem. 2022; 23(17):e202200171.

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Zhang S, Xu Y, Guan H, Cui T, Liao Y, Wei W Nat Commun. 2021; 12(1):2056.

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Holland C, Westfall C, Schaffer J, De Santiago A, Zubieta C, Alvarez S J Biol Chem. 2019; 294(45):16855-16864.

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Cronan J Curr Opin Chem Biol. 2018; 47:60-66.

PMID: 30236800 PMC: 6289770. DOI: 10.1016/j.cbpa.2018.08.004.

References

Jensen K, Niederkruger H, Zimmermann K, Vagstad A, Moldenhauer J, Brendel N . Polyketide proofreading by an acyltransferase-like enzyme. Chem Biol. 2012; 19(3):329-39. PMC: 3400152. DOI: 10.1016/j.chembiol.2012.01.005. View

Gulick A . Conformational dynamics in the Acyl-CoA synthetases, adenylation domains of non-ribosomal peptide synthetases, and firefly luciferase. ACS Chem Biol. 2009; 4(10):811-27. PMC: 2769252. DOI: 10.1021/cb900156h. View

Baldwin A, Berg P . Transfer ribonucleic acid-induced hydrolysis of valyladenylate bound to isoleucyl ribonucleic acid synthetase. J Biol Chem. 1966; 241(4):839-45. View

Loftfield R, EIGNER E . The specificity of enzymic reactions. Aminoacyl-soluble RNA ligases. Biochim Biophys Acta. 1966; 130(2):426-48. DOI: 10.1016/0304-4165(66)90239-x. View

Yadavalli S, Ibba M . Quality control in aminoacyl-tRNA synthesis its role in translational fidelity. Adv Protein Chem Struct Biol. 2012; 86:1-43. DOI: 10.1016/B978-0-12-386497-0.00001-3. View

Martinis S, Boniecki M . The balance between pre- and post-transfer editing in tRNA synthetases. FEBS Lett. 2009; 584(2):455-9. PMC: 2859721. DOI: 10.1016/j.febslet.2009.11.071. View

Bar-Even A, Noor E, Savir Y, Liebermeister W, Davidi D, Tawfik D . The moderately efficient enzyme: evolutionary and physicochemical trends shaping enzyme parameters. Biochemistry. 2011; 50(21):4402-10. DOI: 10.1021/bi2002289. View

Lin S, Cronan J . Closing in on complete pathways of biotin biosynthesis. Mol Biosyst. 2011; 7(6):1811-21. DOI: 10.1039/c1mb05022b. View

Bower S, Perkins J, Yocum R, Howitt C, Rahaim P, Pero J . Cloning, sequencing, and characterization of the Bacillus subtilis biotin biosynthetic operon. J Bacteriol. 1996; 178(14):4122-30. PMC: 178169. DOI: 10.1128/jb.178.14.4122-4130.1996. View

10.

Demoss J, Genuth S, NOVELLI G . THE ENZYMATIC ACTIVATION OF AMINO ACIDS VIA THEIR ACYL-ADENYLATE DERIVATIVES. Proc Natl Acad Sci U S A. 1956; 42(6):325-32. PMC: 528283. DOI: 10.1073/pnas.42.6.325. View

11.

Christensen Q, Cronan J . The Thermoplasma acidophilum LplA-LplB complex defines a new class of bipartite lipoate-protein ligases. J Biol Chem. 2009; 284(32):21317-26. PMC: 2755856. DOI: 10.1074/jbc.M109.015016. View

12.

Loftfield R, Vanderjagt D . The frequency of errors in protein biosynthesis. Biochem J. 1972; 128(5):1353-6. PMC: 1174024. DOI: 10.1042/bj1281353. View

13.

Iram S, Cronan J . The beta-oxidation systems of Escherichia coli and Salmonella enterica are not functionally equivalent. J Bacteriol. 2005; 188(2):599-608. PMC: 1347308. DOI: 10.1128/JB.188.2.599-608.2006. View

14.

Lin S, Hanson R, Cronan J . Biotin synthesis begins by hijacking the fatty acid synthetic pathway. Nat Chem Biol. 2010; 6(9):682-8. PMC: 2925990. DOI: 10.1038/nchembio.420. View