Composition of the Major Elements and Trace Elements of 10 Methanogenic Bacteria Determined by Inductively Coupled Plasma Emission Spectrometry

The elemental composition of 10 methanogenic species was determined by inductively coupled plasma emission spectrometry and by a C-H-N-analyzer. The 10 species were representative of all three orders of the methanogens and were cultivated under defined conditions. Special emphasis was given toMethanosarcina barkeri, represented by 5 strains and cultivated on various substrates. The following elements with the lowest and highest values in parentheses were determined: C (37-44%, w/w), H (5.5-6.5%), N (9.5-12,8%); Na (0.3-4.0%), K (0.13-5.0%), S (0.56-1.2%), P (0.5-2.8%), Ca (order I: 85-550 ppm; order II: 1000-4500 ppm), Mg (0.09-0.53%), Fe (0.07-0.28%), Ni (65-180 ppm), Co (10-120 ppm). Mo (10-70 ppm), Zn (50-630 ppm), Cu (<10-160 ppm), Mn (<5-25 ppm). The biggest variations were found with respect to N and K, which both seem to have important physiological functions. Although it is unknown whether zinc and copper are essential trace elements for methanogens, all investigated species contained remarkably high zinc contents, whereas copper seemed to be present only in some species.