Isolation of a Mouse CDNA Coding for a Developmentally Regulated, Testis-specific Transcript Containing Homeo Box Homology

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1986 Jun 1

PMID 2426103

Citations 28

Authors

D J Wolgemuth

E Engelmyer

R N Duggal

G L Mutter

C Ponzetto

C Viviano

Z F Zakeri

Affiliations

Soon will be listed here.

Abstract

A clone, pHBT-1, containing sequences homologous to Drosophila homeo boxes has been isolated from a mouse testis cDNA library. The sequence is 80% homologous at the DNA level and 88% homologous at the amino acid level to the homeo box sequence of the Antennapedia gene of Drosophila. Sequences flanking the 3' end of the homeo box are highly diverged from other murine homeo box-containing genes characterized to date. RNA blot hybridization analysis of mouse testis poly(A)+ RNA revealed transcripts of approximately 1.4 kb in length. Within the limits of sensitivity of detection of Northern blot analysis, no transcripts were seen in any of the adult somatic tissues examined. Other tissues that contain stem cells, namely those of the hemopoietic system, also lacked detectable amounts of HBT-1 transcripts. HBT-1 transcripts were limited to male germ cell-containing tissues, since RNAs from juvenile and adult ovaries did not contain detectable amounts of the 1.4-kb transcripts. Expression of the HBT-1 gene was not detected in embryonic testes, nor in tests of neonatal animals which contain germ cells up to the Type B stage of spermatogonial development. A role for the expression of the HBT-1 gene in the meiotic stages of male germ cell differentiation is postulated.

Citing Articles

Homeobox genes and male reproductive development.

Lindsey S, Wilkinson M J Assist Reprod Genet. 1996; 13(2):182-92.

PMID: 8688593 DOI: 10.1007/BF02072542.

Homeotic transformation of cervical vertebrae in Hoxa-4 mutant mice.

Horan G, Wu K, Wolgemuth D, Behringer R Proc Natl Acad Sci U S A. 1994; 91(26):12644-8.

PMID: 7809093 PMC: 45495. DOI: 10.1073/pnas.91.26.12644.

Cloning of rat homeobox genes.

Sakoyama Y, Mizuta I, Ogasawara N, Yoshikawa H Biochem Genet. 1994; 32(9-10):351-60.

PMID: 7702549 DOI: 10.1007/BF02426897.

Identification of a testis-specific gene from the mouse t-complex next to a CpG-rich island.

Rappold G, Stubbs L, Labeit S, Crkvenjakov R, Lehrach H EMBO J. 1987; 6(7):1975-80.

PMID: 3653077 PMC: 553585. DOI: 10.1002/j.1460-2075.1987.tb02460.x.

Identification and sequence analysis of a new member of the mouse HSP70 gene family and characterization of its unique cellular and developmental pattern of expression in the male germ line.

Zakeri Z, Wolgemuth D, Hunt C Mol Cell Biol. 1988; 8(7):2925-32.

PMID: 3405224 PMC: 363512. DOI: 10.1128/mcb.8.7.2925-2932.1988.

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