COLD SHOCK DOMAIN PROTEIN 3 is Involved in Salt and Drought Stress Tolerance in Arabidopsis

Overview

Journal FEBS Open Bio

Specialty Biology

Date 2013 Nov 20

PMID 24251108

Citations 24

Authors

Myung-Hee Kim

Shunya Sato

Kentaro Sasaki

Wataru Saburi

Hirokazu Matsui

Ryozo Imai

Affiliations

Soon will be listed here.

Abstract

Cold shock proteins (CSPs) of bacteria are produced in response to cold and function as RNA chaperones that are essential for cold adaptation. Arabidopsis thaliana COLD SHOCK DOMAIN PROTEIN 3 (AtCSP3) shares a domain with bacterial CSPs and is involved in acquisition of freezing tolerance. Our previous study revealed that many of the genes that are down regulated in an AtCSP3 knockout mutant (atcsp3-2) are functionally associated with responses to salt and drought as well as cold. Here, we examined the involvement of AtCSP3 in salt and drought stress tolerance. We found that AtCSP3 is induced during salt and drought stresses, and is regulated by ABA. A knockout mutant of AtCSP3 (atcsp3-2) showed lower survival rates after salt and drought stress treatments. Conversely, the AtCSP3-overexpressing plants displayed higher survival rates after treatment with these stresses. Most of the genes that were down regulated in the atcsp3-2 mutant were found to be inducible upon salt and drought stresses, and upregulated in the AtCSP3-overexpressors. Together, our data demonstrates that AtCSP3 is involved in the regulation of salt and drought stress tolerance in Arabidopsis.

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References

Kim J, Park S, Kwak K, Kim Y, Kim J, Song J . Cold shock domain proteins and glycine-rich RNA-binding proteins from Arabidopsis thaliana can promote the cold adaptation process in Escherichia coli. Nucleic Acids Res. 2006; 35(2):506-16. PMC: 1802614. DOI: 10.1093/nar/gkl1076. View

Nakaminami K, Sasaki K, Kajita S, Takeda H, Karlson D, Ohgi K . Heat stable ssDNA/RNA-binding activity of a wheat cold shock domain protein. FEBS Lett. 2005; 579(21):4887-91. DOI: 10.1016/j.febslet.2005.07.074. View

Li G, Meng X, Wang R, Mao G, Han L, Liu Y . Dual-level regulation of ACC synthase activity by MPK3/MPK6 cascade and its downstream WRKY transcription factor during ethylene induction in Arabidopsis. PLoS Genet. 2012; 8(6):e1002767. PMC: 3386168. DOI: 10.1371/journal.pgen.1002767. View

Karlson D, Imai R . Conservation of the cold shock domain protein family in plants. Plant Physiol. 2003; 131(1):12-5. PMC: 1540277. DOI: 10.1104/pp.014472. View

Castiglioni P, Warner D, Bensen R, Anstrom D, Harrison J, Stoecker M . Bacterial RNA chaperones confer abiotic stress tolerance in plants and improved grain yield in maize under water-limited conditions. Plant Physiol. 2008; 147(2):446-55. PMC: 2409020. DOI: 10.1104/pp.108.118828. View

Liu Q, Kasuga M, Sakuma Y, Abe H, Miura S, Yamaguchi-Shinozaki K . Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression, respectively, in Arabidopsis. Plant Cell. 1998; 10(8):1391-406. PMC: 144379. DOI: 10.1105/tpc.10.8.1391. View

Rossel J, Wilson P, Hussain D, Woo N, Gordon M, Mewett O . Systemic and intracellular responses to photooxidative stress in Arabidopsis. Plant Cell. 2007; 19(12):4091-110. PMC: 2217654. DOI: 10.1105/tpc.106.045898. View

Graumann P, Marahiel M . A superfamily of proteins that contain the cold-shock domain. Trends Biochem Sci. 1998; 23(8):286-90. DOI: 10.1016/s0968-0004(98)01255-9. View

Park S, Kwak K, Oh T, Kim Y, Kang H . Cold shock domain proteins affect seed germination and growth of Arabidopsis thaliana under abiotic stress conditions. Plant Cell Physiol. 2009; 50(4):869-78. DOI: 10.1093/pcp/pcp037. View

10.

Kim M, Sasaki K, Imai R . Cold shock domain protein 3 regulates freezing tolerance in Arabidopsis thaliana. J Biol Chem. 2009; 284(35):23454-60. PMC: 2749119. DOI: 10.1074/jbc.M109.025791. View

11.

Jiang W, Hou Y, Inouye M . CspA, the major cold-shock protein of Escherichia coli, is an RNA chaperone. J Biol Chem. 1997; 272(1):196-202. DOI: 10.1074/jbc.272.1.196. View

12.

Xia B, Ke H, Inouye M . Acquirement of cold sensitivity by quadruple deletion of the cspA family and its suppression by PNPase S1 domain in Escherichia coli. Mol Microbiol. 2001; 40(1):179-88. DOI: 10.1046/j.1365-2958.2001.02372.x. View

13.

Rizhsky L, Davletova S, Liang H, Mittler R . The zinc finger protein Zat12 is required for cytosolic ascorbate peroxidase 1 expression during oxidative stress in Arabidopsis. J Biol Chem. 2004; 279(12):11736-43. DOI: 10.1074/jbc.M313350200. View

14.

Kim M, Sonoda Y, Sasaki K, Kaminaka H, Imai R . Interactome analysis reveals versatile functions of Arabidopsis COLD SHOCK DOMAIN PROTEIN 3 in RNA processing within the nucleus and cytoplasm. Cell Stress Chaperones. 2013; 18(4):517-25. PMC: 3682024. DOI: 10.1007/s12192-012-0398-3. View

15.

Sasaki K, Kim M, Imai R . Arabidopsis COLD SHOCK DOMAIN PROTEIN2 is a RNA chaperone that is regulated by cold and developmental signals. Biochem Biophys Res Commun. 2007; 364(3):633-8. DOI: 10.1016/j.bbrc.2007.10.059. View

16.

Fusaro A, Bocca S, Ramos R, Barroco R, Magioli C, Jorge V . AtGRP2, a cold-induced nucleo-cytoplasmic RNA-binding protein, has a role in flower and seed development. Planta. 2006; 225(6):1339-51. DOI: 10.1007/s00425-006-0444-4. View

17.

Karlson D, Nakaminami K, Toyomasu T, Imai R . A cold-regulated nucleic acid-binding protein of winter wheat shares a domain with bacterial cold shock proteins. J Biol Chem. 2002; 277(38):35248-56. DOI: 10.1074/jbc.M205774200. View

18.

Mittler R, Kim Y, Song L, Coutu J, Coutu A, Ciftci-Yilmaz S . Gain- and loss-of-function mutations in Zat10 enhance the tolerance of plants to abiotic stress. FEBS Lett. 2006; 580(28-29):6537-42. PMC: 1773020. DOI: 10.1016/j.febslet.2006.11.002. View

19.

Bae W, Xia B, Inouye M, Severinov K . Escherichia coli CspA-family RNA chaperones are transcription antiterminators. Proc Natl Acad Sci U S A. 2000; 97(14):7784-9. PMC: 16622. DOI: 10.1073/pnas.97.14.7784. View

20.

Cheng M, Liao P, Kuo W, Lin T . The Arabidopsis ETHYLENE RESPONSE FACTOR1 regulates abiotic stress-responsive gene expression by binding to different cis-acting elements in response to different stress signals. Plant Physiol. 2013; 162(3):1566-82. PMC: 3707555. DOI: 10.1104/pp.113.221911. View