Identification and Expression Analysis of Cold Shock Protein 3 (BcCSP3) in Non-Heading Chinese Cabbage ( Ssp. )

Overview

Journal Plants (Basel)

Date 2020 Jul 18

PMID 32674472

Citations 2

Authors

Feiyi Huang

Jin Wang

Weike Duan

Xilin Hou

Affiliations

Soon will be listed here.

Abstract

A cold-related protein, cold shock protein 3 (BcCSP3), was isolated from non-heading Chinese cabbage in this study. BcCSP3 can encode 205 amino acids (aa) with an open reading frame (ORF) of 618 base pairs (bp). Multiple sequence alignment and phylogenetic tree analyses showed that BcCSP3 contains an N-terminal cold shock domain and is highly similar to AtCSP2, their kinship is recent. Real-time quantitative polymerase chain reaction (RT-qPCR) showed that the expression level of in stems and leaves is higher than that in roots. Compared with other stress treatments, the change in expression level was most pronounced under cold stress. In addition, a fusion protein was localized to the nucleus and cytoplasm. These results indicated that BcCSP3 may play an important role in response to cold stress in non-heading Chinese cabbage. This work may provide a reference for the identification and expression analysis of other genes in non-heading Chinese cabbage.

Citing Articles

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