Plasmalemma- and Tonoplast-ATPase Activity in Mesophyll Protoplasts, Vacuoles and Microsomes of the Crassulacean-acid-metabolism Plant Kalanchoe Daigremontiana

Overview

Journal Planta

Specialty Biology

Date 2013 Nov 15

PMID 24226399

Citations 5

Authors

R A Balsamo

E G Uribe

Affiliations

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Abstract

Adenosine-triphosphatase activity on the plasmalemma and tonoplast of isolated mesophyll protoplasts, isolated vacuoles and tonoplast-derived microsomes of the Crassulacean-acid-metabolism plant Kalanchoe daigremontiana Hamet et Perr., was localized by a cytochemical procedure using lead citrate. Enzyme activity was detected on the cytoplasmic surfaces of the plasmalemma and tonoplast. The identity of the enzymes was confirmed by various treatments differentiating the enzymes by their sensitivity to inhibitors of plasmalemma and tonoplast H(+)-ATPase. Isolated vacuoles and microsomes prepared from isolated vacuoles clearly exhibited single-sided deposition on membrane surfaces.

Citing Articles

Plasmalemma- and tonoplast-ATPase activity in mesophyll protoplasts, vacuoles and microsomes of the Crassulacean-acid-metabolism plant Kalanchoe daigremontiana.

Balsamo R, Uribe E Planta. 2013; 173(2):190-6.

PMID: 24226399 DOI: 10.1007/BF00403010.

Leaf anatomy and ultrastructure of the Crassulacean-acid-metabolism plant Kalanchoe daigremontiana.

Balsamo R, Uribe E Planta. 2013; 173(2):183-9.

PMID: 24226398 DOI: 10.1007/BF00403009.

Phase resetting of the circadian rhythm of carbon dioxide assimilation inBryophyllum leaves in relation to their malate content following brief exposure to high and low temperatures, darkness and 5% carbon dioxide.

Anderson C, Wilkins M Planta. 2013; 180(1):61-73.

PMID: 24201845 DOI: 10.1007/BF02411411.

Proton and anion transport at the tonoplast in crassulacean-acid-metabolism plants: specificity of the malate-influx system in Kalanchoë daigremontiana.

White P, Smith J Planta. 2013; 179(2):265-74.

PMID: 24201527 DOI: 10.1007/BF00393698.

Isolation of mesophyll and secretory cell protoplasts of the halophyte Ceratostigma plumbaginoides (L.): a comparison of ATPase concentration and activity.

Balsamo R, Thomson W Plant Cell Rep. 2013; 15(6):418-22.

PMID: 24178421 DOI: 10.1007/BF00232067.

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