HIV-1 Nucleocapsid Proteins As Molecular Chaperones for Tetramolecular Antiparallel G-quadruplex Formation

Overview

Journal J Am Chem Soc

Publisher American Chemical Society

Specialty Chemistry

Date 2013 Nov 15

PMID 24224650

Citations 19

Authors

Arivazhagan Rajendran

Masayuki Endo

Kumi Hidaka

Phong Lan Thao Tran

Jean-Louis Mergny

Robert J Gorelick

Hiroshi Sugiyama

Affiliations

Soon will be listed here.

Abstract

HIV-1 nucleocapsid proteins (NCps) facilitate remodeling of nucleic acids to fold thermodynamically stable conformations, and thus called nucleic acid chaperones. To date only little is known on the stoichiometry, NCp-NCp interactions, chaperone activity on G-quadruplex formation, and so on. We report here the direct and real-time analysis on such properties of proteolytic intermediate NCp15 and mature NCp7 using DNA origami. The protein particles were found to predominantly exist in monomeric form, while dimeric and multimeric forms were also observed both in free solution and bound to the quadruplex structure. The formation and the dissociation events of the G-quadruplexes were well documented in real-time and the intermediate-like states were also visualized. We anticipate that this pioneering study will strengthen our understanding on the chaperone activity of HIV-1 proteins which in turn will be helpful for the drug design based on G-quadruplex and also for the development of drugs against AIDS.

Citing Articles

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