Complementation of the Amylose-free Starch Mutant of Potato (Solanum Tuberosum.) by the Gene Encoding Granule-bound Starch Synthase

Overview

Journal Theor Appl Genet

Publisher Springer

Specialty Genetics

Date 2013 Nov 12

PMID 24213172

Citations 8

Authors

F R van der Leij

R G F Visser

K Oosterhaven

D A van der Kop

E Jacobsen

W J Feenstra

Affiliations

Soon will be listed here.

Abstract

Agrobacterium rhizogenes-mediated introduction of the wild-type allele of the gene encoding granulebound starch synthase (GBSS) into the amylose-free starch mutantamf of potato leads to restoration of GBSS activity and amylose synthesis, which demonstrates thatAmf is the structural gene for GBSS. Amylose was found in columella cells of root tips, in stomatal guard cells, tubers, and pollen, while in the control experiments using only vector DNA, these tissues remained amylose free. This confirms the fact that, in potato, GBSS is the only enzyme responsible for the presence of amylose, accumulating in all starch-containing tissues. Amylose-containing transformants showed no positive correlation between GBSS activity and amylose content, which confirms that the former is not the sole regulating factor in amylose metabolism.

Citing Articles

High frequency of tetraploidy in Agrobacterium-mediated transformants regenerated from tuber discs of diploid potato lines.

Imai T, Aida R, Ishige T Plant Cell Rep. 2013; 12(6):299-302.

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Antisense regulation of the rice waxy gene expression using a PCR-amplified fragment of the rice genome reduces the amylose content in grain starch.

Shimada H, Tada Y, Kawasaki T, Fujimura T Theor Appl Genet. 2013; 86(6):665-72.

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Expression of a wild-type GBSS gene introduced into an amylose-free potato mutant by Agrobacterium tumefaciens and the inheritance of the inserts at the microsporic level.

Flipse E, Huisman J, De Vries B, Bergervoet J, Jacobsen E, Visser R Theor Appl Genet. 2013; 88(3-4):369-75.

PMID: 24186021 DOI: 10.1007/BF00223647.

Gene silencing in potato: allelic differences and effect of ploidy.

Wolters A, Visser R Plant Mol Biol. 2000; 43(2-3):377-86.

PMID: 10999417 DOI: 10.1023/a:1006476621946.

GBSS T-DNA inserts giving partial complementation of the amylose-free potato mutant can also cause co-suppression of the endogenous GBSS gene in a wild-type background.

Flipse E, Kuipers A, Jacobsen E, Visser R Plant Mol Biol. 1996; 31(4):731-9.

PMID: 8806404 DOI: 10.1007/BF00019461.

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