Molecular Cloning of the Mouse Cell Adhesion Molecule Uvomorulin: CDNA Contains a B1-related Sequence

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1986 Mar 1

PMID 2419906

Citations 14

Authors

R Schuh

D Vestweber

I Riede

M Ringwald

U B Rosenberg

H Jackle

R Kemler

Affiliations

Soon will be listed here.

Abstract

A clone (F20) containing coding sequences for the cell adhesion molecule uvomorulin was isolated by immunological techniques from cDNA library in the expression vector lambda gt11. The beta-galactosidase-uvomorulin fusion protein was used to affinity purify anti-uvomorulin antibodies. Affinity-purified antibodies recognized uvomorulin from cell lysates of embryonal carcinoma cells and reacted with the cell surface of embryonal carcinoma cells. The 1.8-kilobase cDNA insert hybridized to a single 4.3-kilobase poly(A)+ RNA species found only in cells expressing uvomorulin. Part of the nontranslated 3' sequences of the cloned uvomorulin cDNA is homologous to the interspersed B1 repeat of the mouse genome.

Citing Articles

The E-Cadherin Cleavage Associated to Pathogenic Bacteria Infections Can Favor Bacterial Invasion and Transmigration, Dysregulation of the Immune Response and Cancer Induction in Humans.

Devaux C, Mezouar S, Mege J Front Microbiol. 2019; 10:2598.

PMID: 31781079 PMC: 6857109. DOI: 10.3389/fmicb.2019.02598.

Cadherin Signaling in Cancer: Its Functions and Role as a Therapeutic Target.

Yu W, Yang L, Li T, Zhang Y Front Oncol. 2019; 9:989.

PMID: 31637214 PMC: 6788064. DOI: 10.3389/fonc.2019.00989.

Distinct subcellular localization of E-cadherin between epithelioid angiomyolipoma and triphasic angiomyolipoma: A preliminary case-control study.

Bi X, Guo L, Wang X, Wei Q, Du Q, Jiang W Oncol Lett. 2017; 14(1):695-704.

PMID: 28693223 PMC: 5494718. DOI: 10.3892/ol.2017.6272.

Should I stay or should I go? Cadherin function and regulation in the neural crest.

Taneyhill L, Schiffmacher A Genesis. 2017; 55(6).

PMID: 28253541 PMC: 5468476. DOI: 10.1002/dvg.23028.

Discovering the molecular components of intercellular junctions--a historical view.

Franke W Cold Spring Harb Perspect Biol. 2010; 1(3):a003061.

PMID: 20066111 PMC: 2773636. DOI: 10.1101/cshperspect.a003061.

References

Berstine E, Hooper M, Grandchamp S, EPHRUSSI B . Alkaline phosphatase activity in mouse teratoma. Proc Natl Acad Sci U S A. 1973; 70(12):3899-903. PMC: 427353. DOI: 10.1073/pnas.70.12.3899. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Vestweber D, Kemler R . Rabbit antiserum against a purified surface glycoprotein decompacts mouse preimplantation embryos and reacts with specific adult tissues. Exp Cell Res. 1984; 152(1):169-78. DOI: 10.1016/0014-4827(84)90241-6. View

Ofverstedt L, Hammarstrom K, Balgobin N, Hjerten S, Pettersson U, Chattopadhyaya J . Rapid and quantitative recovery of DNA fragments from gels by displacement electrophoresis (isotachophoresis). Biochim Biophys Acta. 1984; 782(2):120-6. DOI: 10.1016/0167-4781(84)90014-9. View

Young R, Davis R . Efficient isolation of genes by using antibody probes. Proc Natl Acad Sci U S A. 1983; 80(5):1194-8. PMC: 393560. DOI: 10.1073/pnas.80.5.1194. View

Nicolas J, Avner P, Gaillard J, Guenet J, Jakob H, Jacob F . Cell lines derived from teratocarcinomas. Cancer Res. 1976; 36(11 Pt. 2):4224-31. View

Rutishauser U, Thiery J, Brackenbury R, Sela B, Edelman G . Mechanisms of adhesion among cells from neural tissues of the chick embryo. Proc Natl Acad Sci U S A. 1976; 73(2):577-81. PMC: 335953. DOI: 10.1073/pnas.73.2.577. View

Kessler S . Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. J Immunol. 1975; 115(6):1617-24. View

Hyafil F, Morello D, Babinet C, Jacob F . A cell surface glycoprotein involved in the compaction of embryonal carcinoma cells and cleavage stage embryos. Cell. 1980; 21(3):927-34. DOI: 10.1016/0092-8674(80)90456-0. View

10.

Messing J, Vieira J . A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene. 1982; 19(3):269-76. DOI: 10.1016/0378-1119(82)90016-6. View

11.

Peyrieras N, Hyafil F, Louvard D, Ploegh H, Jacob F . Uvomorulin: a nonintegral membrane protein of early mouse embryo. Proc Natl Acad Sci U S A. 1983; 80(20):6274-7. PMC: 394278. DOI: 10.1073/pnas.80.20.6274. View

12.

Ocklind C, Forsum U, Obrink B . Cell surface localization and tissue distribution of a hepatocyte cell-cell adhesion glycoprotein (cell-CAM 105). J Cell Biol. 1983; 96(4):1168-71. PMC: 2112309. DOI: 10.1083/jcb.96.4.1168. View

13.

Vestweber D, Kemler R . Some structural and functional aspects of the cell adhesion molecule uvomorulin. Cell Differ. 1984; 15(2-4):269-73. DOI: 10.1016/0045-6039(84)90084-8. View

14.

Damsky C, Richa J, Solter D, Knudsen K, Buck C . Identification and purification of a cell surface glycoprotein mediating intercellular adhesion in embryonic and adult tissue. Cell. 1983; 34(2):455-66. DOI: 10.1016/0092-8674(83)90379-3. View

15.

Steinmetz M, Moore K, Frelinger J, Sher B, Shen F, Boyse E . A pseudogene homologous to mouse transplantation antigens: transplantation antigens are encoded by eight exons that correlate with protein domains. Cell. 1981; 25(3):683-92. DOI: 10.1016/0092-8674(81)90175-6. View

16.

Gallin W, Edelman G, Cunningham B . Characterization of L-CAM, a major cell adhesion molecule from embryonic liver cells. Proc Natl Acad Sci U S A. 1983; 80(4):1038-42. PMC: 393523. DOI: 10.1073/pnas.80.4.1038. View

17.

Kemler R, Babinet C, Eisen H, Jacob F . Surface antigen in early differentiation. Proc Natl Acad Sci U S A. 1977; 74(10):4449-52. PMC: 431960. DOI: 10.1073/pnas.74.10.4449. View

18.

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

19.

Nicolas J, Dubois P, Jakob H, Gaillard J, Jacob F . [Mouse teratocarcinoma: differentiation in cultures of a multipotential primitive cell line (author's transl)]. Ann Microbiol (Paris). 1975; 126(1):3-22. View

20.

Boller K, Vestweber D, Kemler R . Cell-adhesion molecule uvomorulin is localized in the intermediate junctions of adult intestinal epithelial cells. J Cell Biol. 1985; 100(1):327-32. PMC: 2113467. DOI: 10.1083/jcb.100.1.327. View