RAPDs As an Aid to Evaluate the Genetic Integrity of Somatic Embryogenesis-derived Populations of Picea Mariana (Mill.) B.S.P

Overview

Journal Theor Appl Genet

Publisher Springer

Specialty Genetics

Date 2013 Nov 7

PMID 24193386

Citations 20

Authors

N Isabel

L Tremblay

M Michaud

F M Tremblay

J Bousquet

Affiliations

Soon will be listed here.

Abstract

The usefulness of random amplified polymorphic DNA (RAPD) in assessing the genetic stability of somatic embryogenesis-derived populations of black spruce [Picea mariana (Mill.) B.S.P.] was evaluated. Three arbitrary 11-mer primers were successfully used to amplify DNA from both in-vivo and in-vitro material. Twenty-five embryogenic cell lines, additional zygotic embryos and megagametophytes from three controlled crosses involving four selected genotypes of black spruce were used for the segregation analysis of RAPD variants. Ten markers were genetically characterized and used to evaluate the genetic stability of somatic embryos derived from three embryogenic cell lines (one cell line per cross, 30 somatic embryos per cell line). No variation was detected within clones. The utilization of RAPD markers both for the assessment of genetic stability of clonal materials and to certify genetic stability throughout the process of somatic embryogenesis is discussed.

Citing Articles

An assessment for in vitro propagation and genetic stability of Phoebe goalparensis Hutchinson, an endemic valuable timber tree of North East India.

Mudoi K, Gogoi B, Borah G, Hussain M, Tasfia T, Borah K J Genet Eng Biotechnol. 2023; 21(1):29.

PMID: 36897435 PMC: 10006360. DOI: 10.1186/s43141-023-00487-9.

Random Amplified Polymorphic DNA (RAPD) and Derived Techniques.

Babu K, Sheeja T, Minoo D, Rajesh M, Samsudeen K, Suraby E Methods Mol Biol. 2020; 2222:219-247.

PMID: 33301097 DOI: 10.1007/978-1-0716-0997-2_13.

RAPD polymorphisms among variant and phenotypically normal rice (Oryza sativa var.indica) somaclonal progenies.

Godwin I, Sangduen N, Kunanuvatchaidach R, Piperidis G, Adkins S Plant Cell Rep. 2019; 16(5):320-324.

PMID: 30727671 DOI: 10.1007/BF01088289.

RAPD analysis of somaclonal variants derived from embryo callus cultures of peach.

Hashmi G, Huettel R, Meyer R, Krusberg L, Hammerschlag F Plant Cell Rep. 2019; 16(9):624-627.

PMID: 30727607 DOI: 10.1007/BF01275503.

Development of a Traceability System Based on a SNP Array for Large-Scale Production of High-Value White Spruce ().

Godbout J, Tremblay L, Levasseur C, Lavigne P, Rainville A, MacKay J Front Plant Sci. 2017; 8:1264.

PMID: 28791035 PMC: 5524734. DOI: 10.3389/fpls.2017.01264.

References

Breiman A, Rotem-Abarbanell D, Karp A, Shaskin H . Heritable somaclonal variation in wild barley (Hordeum spontaneum). Theor Appl Genet. 2013; 74(1):104-12. DOI: 10.1007/BF00290092. View

Deragon J, Landry B . RAPD and other PCR-based analyses of plant genomes using DNA extracted from small leaf disks. PCR Methods Appl. 1992; 1(3):175-80. DOI: 10.1101/gr.1.3.175. View

Litvay J, Verma D, Johnson M . Influence of a loblolly pine (Pinus taeda L.). Culture medium and its components on growth and somatic embryogenesis of the wild carrot (Daucus carota L.). Plant Cell Rep. 2013; 4(6):325-8. DOI: 10.1007/BF00269890. View

Welsh J, Petersen C, McClelland M . Polymorphisms generated by arbitrarily primed PCR in the mouse: application to strain identification and genetic mapping. Nucleic Acids Res. 1991; 19(2):303-6. PMC: 333594. DOI: 10.1093/nar/19.2.303. View

Carlson J, Tulsieram L, Glaubitz J, Luk V, Kauffeldt C, Rutledge R . Segregation of random amplified DNA markers in F1 progeny of conifers. Theor Appl Genet. 2013; 83(2):194-200. DOI: 10.1007/BF00226251. View

Hu J, Quiros C . Identification of broccoli and cauliflower cultivars with RAPD markers. Plant Cell Rep. 2013; 10(10):505-11. DOI: 10.1007/BF00234583. View

Eastman P, Webster F, Pitel J, Roberts D . Evaluation of somaclonal variation during somatic embryogenesis of interior spruce (Picea glauca engelmannii complex) using culture morphology and isozyme analysis. Plant Cell Rep. 2013; 10(8):425-30. DOI: 10.1007/BF00232617. View

Hartmann C, Henry Y, De Buyser J, Aubry C, Rode A . Identification of new mitochondrial genome organizations in wheat plants regenerated from somatic tissue cultures. Theor Appl Genet. 2013; 77(2):169-75. DOI: 10.1007/BF00266182. View

Peschke V, Phillips R, Gengenbach B . Discovery of transposable element activity among progeny of tissue culture--derived maize plants. Science. 1987; 238(4828):804-7. DOI: 10.1126/science.238.4828.804. View

10.

G Williams J, Kubelik A, Livak K, Rafalski J, Tingey S . DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 1990; 18(22):6531-5. PMC: 332606. DOI: 10.1093/nar/18.22.6531. View

11.

Shirzadegan M, Palmer J, Christey M, Earle E . Patterns of mitochondrial DNA instability in Brassica campestris cultured cells. Plant Mol Biol. 1991; 16(1):21-37. DOI: 10.1007/BF00017914. View

12.

Giovannoni J, Wing R, Ganal M, Tanksley S . Isolation of molecular markers from specific chromosomal intervals using DNA pools from existing mapping populations. Nucleic Acids Res. 1991; 19(23):6553-8. PMC: 329217. DOI: 10.1093/nar/19.23.6553. View

13.

Muller E, Brown P, Hartke S, Lorz H . DNA variation in tissue-culture-derived rice plants. Theor Appl Genet. 2013; 80(5):673-9. DOI: 10.1007/BF00224228. View

14.

Roy A, Frascaria N, Mackay J, Bousquet J . Segregating random amplified polymorphic DNAs (RAPDs) in Betula alleghaniensis. Theor Appl Genet. 2013; 85(2-3):173-80. DOI: 10.1007/BF00222856. View

15.

Brettell R, Pallotta M, Gustafson J, Appels R . Variation at the Nor loci in triticale derived from tissue culture. Theor Appl Genet. 2013; 71(4):637-43. DOI: 10.1007/BF00264268. View

16.

Dunford R, Walden R . Plastid genome structure and plastid-related transcript levels in albino barley plants derived from another culture. Curr Genet. 1991; 20(4):339-47. DOI: 10.1007/BF00318524. View

17.

Michelmore R, Paran I, Kesseli R . Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci U S A. 1991; 88(21):9828-32. PMC: 52814. DOI: 10.1073/pnas.88.21.9828. View

18.

Larkin P, Scowcroft W . Somaclonal variation - a novel source of variability from cell cultures for plant improvement. Theor Appl Genet. 2013; 60(4):197-214. DOI: 10.1007/BF02342540. View

19.

Peschke V, Phillips R . Activation of the maize transposable element Suppressor-mutator (Spm) in tissue culture. Theor Appl Genet. 2013; 81(1):90-7. DOI: 10.1007/BF00226117. View

20.

Larkin P, Ryan S, Brettell R, Scowcroft W . Heritable somaclonal variation in wheat. Theor Appl Genet. 2013; 67(5):443-55. DOI: 10.1007/BF00263410. View