Article: Cloning of photoreactivation repair gene and excision repair gene of the yeast Saccharomyces cerevisiae

The photoreactivation repair gene (PHR1) of the yeast Saccharomyces cerevisiae was cloned in a hybrid plasmid (pJDB207), which is able to replicate as a multicopy episome in S. cerevisiae and Escherichia coli cells. The size of the DNA fragment found to have the photoreactivation activity was 3.0 kb, determined by recloning of the isolated fragment. In wild type cells transformed by the plasmid containing the PHR1 gene, the number of DNA photolyase molecules was 15 times greater than in wild type cells with pJDB207 only. Using the same receptor strain the excision repair gen RAD1 was also isolated. The size of the insert of the DNA which complements excision repair deficiency in recipient yeast cells was 5.7 kb. The recipient cells after transformation with the plasmid containing RAD1 showed the same UV-sensitivty as wild type cells with pJDB207 only.

Citing Articles

Photoreactivation of UV-induced cyclobutane pyrimidine dimers in the MFA2 gene of Saccharomyces cerevisiae.

Morse N, Meniel V, Waters R Nucleic Acids Res. 2002; 30(8):1799-807.

PMID: 11937634 PMC: 113223. DOI: 10.1093/nar/30.8.1799.

Light-illumination effects on the cellular concentration of photolyase molecules in yeast.

Fukui A, Laskowski W Radiat Environ Biophys. 1985; 24(4):251-8.

PMID: 3909207 DOI: 10.1007/BF01210932.

DNA repair genes of Saccharomyces cerevisiae: complementing rad4 and rev2 mutations by plasmids which cannot be propagated in Escherichia coli.

Siede W, Eckardt-Schupp F Curr Genet. 1986; 11(3):205-10.

PMID: 3329049 DOI: 10.1007/BF00420608.

Expression of an Escherichia coli phr gene in the yeast Saccharomyces cerevisiae.

Langeveld S, Yasui A, Eker A Mol Gen Genet. 1985; 199(3):396-400.

PMID: 2993790 DOI: 10.1007/BF00330748.

References

1.

CHEVALLIER M, Bloch J, Lacroute F . Transcriptional and translational expression of a chimeric bacterial-yeast plasmid in yeasts. Gene. 1980; 11(1-2):11-9. DOI: 10.1016/0378-1119(80)90082-7. View

2.

Reynolds R, Love J, Friedberg E . Molecular mechanisms of pyrimidine dimer excision in Saccharomyces cerevisiae: excision of dimers in cell extracts. J Bacteriol. 1981; 147(2):705-8. PMC: 216098. DOI: 10.1128/jb.147.2.705-708.1981. View

3.

HANAWALT P, Cooper P, Ganesan A, Smith C . DNA repair in bacteria and mammalian cells. Annu Rev Biochem. 1979; 48:783-836. DOI: 10.1146/annurev.bi.48.070179.004031. View

4.

Clewell D . Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol. J Bacteriol. 1972; 110(2):667-76. PMC: 247463. DOI: 10.1128/jb.110.2.667-676.1972. View

5.

Struhl K, Stinchcomb D, Scherer S, Davis R . High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules. Proc Natl Acad Sci U S A. 1979; 76(3):1035-9. PMC: 383183. DOI: 10.1073/pnas.76.3.1035. View

Cloning of Photoreactivation Repair Gene and Excision Repair Gene of the Yeast Saccharomyces Cerevisiae