Changes in Extrinsic Fluorescence Intensity of the Electroplax Membrane During Electrical Excitation

Overview

Journal J Membr Biol

Date 2013 Nov 1

PMID 24172986

Citations 3

Authors

J Patrick

B Valeur

L Monnerie

J P Changeux

Affiliations

Soon will be listed here.

Abstract

The fluorescent dye 1-anilinonapthalene-8-sulfonic acid (ANS) has been used as a probe of changes in membrane conformation accompanying excitation of the electroplax ofElectrophorus electricus. ANS binds reversibly to the excitable membrane at rest. During generation of an action potential, an increase in ANS-fluorescence intensity is observed which resembles but does not strictly follow the membrane potential. Experiments using the current-clamp technique have demonstrated a linear relationship between the change in membrane potential and the change in ANS fluorescence intensity. The change in fluorescence intensity is not a consequence of binding to membrane sites of increased affinity nor of an electrophoretic concentration of ANS molecules at the membrane surface.It is not known whether the change in fluorescence intensity is due to a change in quantum yield of bound ANS or to an increase in the amount of bound ANS. In either case, the change in fluorescence intensity may be interpreted as a change in membrane conformation.

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References

Cohen L, Keynes R, Hille B . Light scattering and birefringence changes during nerve activity. Nature. 1968; 218(5140):438-41. DOI: 10.1038/218438a0. View

Kasai M, Podleski T . Some structural properties of excitable membranes labelled by fluorescent probes. FEBS Lett. 1970; 7(1):13-19. DOI: 10.1016/0014-5793(70)80605-6. View

Valeur B, Patrick J, Podleski T, Monnerie L, Changeux J . [Perfecting an apparatus allowing to measure changes of extrinsic fluorescence intensit y associated with electric excitation of electroplaque isolated from gymnotus]. C R Acad Hebd Seances Acad Sci D. 1970; 270(21):2583-6. View

Morlock M, Benamy D, Grundfest H . Analysis of spike electrogenesis of Eel electroplaques with phase plane and impedance measurements. J Gen Physiol. 1968; 52(1):22-45. PMC: 2225796. DOI: 10.1085/jgp.52.1.22. View

Stryer L . The interaction of a naphthalene dye with apomyoglobin and apohemoglobin. A fluorescent probe of non-polar binding sites. J Mol Biol. 1965; 13(2):482-95. DOI: 10.1016/s0022-2836(65)80111-5. View

Schoffeniels E . An isolated single electroplax preparation. II. Improved preparation for studying ion flux. Biochim Biophys Acta. 1957; 26(3):585-96. DOI: 10.1016/0006-3002(57)90106-3. View

Kasai M, Changeux J, Monnerie L . In vitro interaction of 1-anilino 8 naphthalene sulfonate with excitable membranes isolated from the electric organ of Electrophorus electricus. Biochem Biophys Res Commun. 1969; 36(3):420-7. DOI: 10.1016/0006-291x(69)90581-6. View

Weber G, Young L . FRAGMENTATION OF BOVINE SERUM ALBUMIN BY PEPSIN. I. THE ORIGIN OF THE ACID EXPANSION OF THE ALBUMIN MOLECULE. J Biol Chem. 1964; 239:1415-23. View

Keynes R, MARTINS-FERREIRA H . Membrane potentials in the electroplates of the electric eel. J Physiol. 1953; 119(2-3):315-51. PMC: 1392804. DOI: 10.1113/jphysiol.1953.sp004849. View

10.

Jain M, MARKS R, CORDES E . Kinetic model of conduction changes across excitable membranes. Proc Natl Acad Sci U S A. 1970; 67(2):799-806. PMC: 283276. DOI: 10.1073/pnas.67.2.799. View

11.

Ruarte A, Schwartz T, Grundfest H . Potassium inactivation and impedance changes during spike electrogenesis in eel electroplaques. J Gen Physiol. 1970; 55(1):33-47. PMC: 2202964. DOI: 10.1085/jgp.55.1.33. View

12.

Cohen L, Hille B, Keynes R . Light scattering and birefringence changes during activity in the electric organ of electrophorus electricus. J Physiol. 1969; 203(2):489-509. PMC: 1351457. DOI: 10.1113/jphysiol.1969.sp008876. View

13.

Nakamura Y, Nakajima S, Grundfest H . Analysis of Spike Electrogenesis and Depolarizing K Inactivation in Electroplaques of Electrophorus electricus, L. J Gen Physiol. 2009; 49(2):321-49. PMC: 2195482. DOI: 10.1085/jgp.49.2.321. View

14.

Conti F, Tasaki I . Changes in extrinsic fluorescence in squid axons during voltage-clamp. Science. 1970; 169(3952):1322-4. DOI: 10.1126/science.169.3952.1322. View

15.

Tasaki I, Carnay L, Sandlin R, Watanabe A . Fluorescence changes during conduction in nerves stained with Acridine Orange. Science. 1969; 163(3868):683-5. DOI: 10.1126/science.163.3868.683. View

16.

Adam G . [Theory of nerve excitation as cooperative cation exchange in a 2-dimensional lattice. I. Ion flow following a depolarizing discontinuity in the membrane potential]. Z Naturforsch B. 1968; 23(2):181-97. View

17.

Morrow T, Salmon G, Frankevich E . Effect of an electric field on the radiation induced fluorescence from solutions of aromatic hydrocarbons in cyclohexane. Nature. 1968; 219(5153):481-2. DOI: 10.1038/219481a0. View

18.

Tasaki I, Watanabe A, Sandlin R, Carnay L . Changes in fluorescence, turbidity, and birefringence associated with nerve excitation. Proc Natl Acad Sci U S A. 1968; 61(3):883-8. PMC: 305410. DOI: 10.1073/pnas.61.3.883. View

19.

Hill T . Electric fields and the cooperativity of biological membranes. Proc Natl Acad Sci U S A. 1967; 58(1):111-4. PMC: 335604. DOI: 10.1073/pnas.58.1.111. View

20.

Blumenthal R, Changeux J, Lefever R . Membrane excitability and dissipative instabilities. J Membr Biol. 2013; 2(1):351-74. DOI: 10.1007/BF01869870. View